ОТРИМАННЯ TAQ-ПОЛІМЕРАЗИ В КУЛЬТУРІ ESCHERICHIA COLI
Ключові слова:
PCR, recombinant protein, E.сoli, Taq DNA polymeraseАнотація
The constructs and methods for analyzing the obtained recombinant protein of Taq polymerase are described in this work. The obtained Taq polymerase, with a mass of 94 kDa and free of DNA nuclease contaminants, exhibited a purity exceeding 90% and an activity level comparable to that of the commercial enzyme sample.
Посилання
Miura, Masashi; Tanigawa, Chihiro; Fujii, Yoshito; Kaneko, Satoshi (2013). Comparison Of Six Commercially-Available Dna Polymerases For Direct Pcr. Revista do Instituto de Medicina Tropical de São Paulo, 55(6), 401–406. doi:10.1590/S0036-46652013000600005
Wu, S., Beard, W. A., Pedersen, L. G., & Wilson, S. H. (2013). Structural Comparison of DNA Polymerase Architecture Suggests a Nucleotide Gateway to the Polymerase Active Site. Chemical Reviews, 114(5), 2759–2774. doi:10.1021/cr3005179
Nosaibah Samman, Khawlah Al-Muhalhil, Atef Nehdi, «A simple and efficient method for Taq DNA polymerase purification based on heat denaturation and affinity chromatography, Journal of King Saud University - Science, Volume 35, Issue 3, 2023, 102565, ISSN 1018-3647, https://doi.org/10.1016/j.jksus.2023.102565.
