СТВОРЕННЯ КОНСТРУКЦІЙ ДЛЯ БАГАТОКОПІЙНОЇ ІНТЕГРАЦІЇ ГЕНІВ ІНТЕРЕСУ В ДЕЛЬТА ПОСЛІДОВНОСТІ ГЕНОМУ ДРІЖДЖІВ
Ключові слова:
S. cerevisiae, CRISPR/Cas9, delta integration, genome engineeringАнотація
Yeasts are attractive cell factories for recombinant protein production. Stable integration and high-copy number of gene interest are essential for strain productivity. Constructions designed in this work are intended for CRISPR-mediated multi-copy integrations into delta-sites of Saccharomyces cerevisiae genome.
Посилання
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