ПРАКТИЧНЕ ВИКОРИСТАННЯ КОНТРОЛЮ РОЗЧИНЕНОГО КИСНЮ ПІД ЧАС КУЛЬТИВУВАННЯ РЕКОМБІНАНТНОГО ШТАМУ ESCHERICHIA COLI ПРИ ВИРОБНИЦТВІ ІНСУЛІНУ ЛЮДИНИ
Ключові слова:
dissolved oxygen, fermenter reactor, insulin biosynthesis, total protein, refolded hybrid proteinАнотація
The article describes the observation of the behavior of dissolved oxygen during the cultivation of Escherichia coli cells and the possibility of further selection of components for further insulin synthesis depending on the type of dissolved oxygen behavior during each specific cultivation. The practical use of observing the behavior of dissolved oxygen, depending on its nature, in industrial applications, will increase the yield of the target product and simplify the further synthesis process.
Посилання
Nabih A. Baeshen Mohammed N. Baeshen Abdullah Sheikh Roop S. Bora Mohamed Morsi M. Ahmed Hassan A. I. Ramadan Kulvinder Singh Saini and Elrashdy M. Redwan. Cell factories for insulin production. BioMed Central. 2014. P. 2–3.
М.Г. Зінченко Біохімічні і мікробіологічні основи харчової та бродильної техноло-гії : навч. посібник / М. Г. Зінченко. – Харків : НТУ «ХПІ», 2009. С. 55 – 56.
Yaoguang Wei Yisha Jiao Dong An Daoliang Li Qiong Wei. Review of Dissolved Oxygen Detection Technology: From Laboratory Analysis to Online Intelligent Detection. MDPI. 2019. P. 2–14.
Andreas Schmideder Dirk Weuster Botz. High performance recombinant protein production with Escherichia coli in continuously operated cascades of stirred tank reactors. Society for Industrial Microbiology and Biotechnology. 2017. P. 1022–1025.
Luciana Gomes Gabriel Monteiro and Filipe Mergulhão. The Impact of IPTG Induction on Plasmid Stability and Heterologous Protein Expression by Escherichia coli Biofilms. MDPI. 2019. P. 2–4.
Yongxue Ding You Pan Mark Gibson Troy McSherry and Steven P. Allen. Oxygen Control Strategy and Yield of Recombinant Antibody Fragments Produced in Fermentation. MDPI. 2019. P. 1–3.
Hans Peter Sørensen1 and Kim Kusk Mortensen. Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. BioMed Central. 2005. P. 4–5.
Maria K. Eiberle Alois Jungbauer. Technical Refolding of Proteins: Do we have Freedom to Operate?. Biotechnology Journal. 2010. P. 5–15.
Hiroshi Yamaguchi 1. and Masaya Miyazaki. Refolding Techniques for Recovering Biologically Active Recombinant Proteins from Inclusion Bodies. Biomolecules. 2014. P. 242–247.
Nan Wang Kai Ren Rong Jia Wenting Chen and Ruirui Sun. Expression of a fungal manganese peroxidase in Escherichia coli: a comparison between the soluble and refolded enzymes. BMC Biotechnology. 2016. P. 4–12.
