ARTEMISIA VULGARIS “HAIRY” ROOTS AS A SOURCE OF SAFE ANTIOXIDANT COMPOUNDS

Автор(и)

  • A. I. Olkhovska ECS “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv; Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Україна
  • Y. V. Zahorska ECS “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv, Україна
  • M. I. Chopei ECS “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv, Україна
  • K. S. Afanasieva ECS “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv, Україна
  • N. A. Matvieieva Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Україна

Ключові слова:

Artemisia vulgaris L., “hairy” roots, flavonoids, antioxidants, genotoxicity

Анотація

The present study aimed to investigate flavonoid content and antioxidant activity of Artemisia vulgaris “hairy” roots of 3 lines. We also studied the safety of transgenic root extracts by determining their genotoxicity. The total flavonoid content in water extracts of the fresh “hairy” roots reached up to 1.976 ± 0.1 mg/g FW, which is 4.8 times higher than in control plant roots. Extracts of transgenic roots on average showed 1.5 times higher antioxidant activity compared to untransformed roots. The comet assay results indicated the absence of the genotoxic effect of all tested extracts. Therefore, these extracts can be the source of safe antioxidant compounds.

Посилання

Anibogwu, Rosemary, et al. "Extraction, isolation and characterization of bioactive compounds from Artemisia and their biological significance: A review." Molecules 26.22 (2021): 6995. https://doi.org/10.3390/molecules26226995

Adwas, Almohktar A., et al. "Oxidative stress and antioxidant mechanisms in human body." J. Appl. Biotechnol. Bioeng 6.1 (2019): 43-47. https://doi.org/10.15406/jabb.2019.06.00173

Gantait, Saikat, and Eashan Mukherjee. "Hairy root culture technology: applications, constraints and prospect." Applied Microbiology and Biotechnology 105 (2021): 35-53. https://doi.org/10.1007/s00253-020-11017-9

Matvieieva, N., et al. "Alterations in the antioxidant status of transgenic roots of Artemisia spp. representatives after A. rhizogenes-mediated genetic transformation." Cytology and genetics 52 (2018): 253-259. https://doi.org/10.3103/S0095452718040060

Pękal, Anna, and Krystyna Pyrzynska. "Evaluation of aluminium complexation reaction for flavonoid content assay." Food Analytical Methods 7 (2014): 1776-1782. https://doi.org/10.1007/s12161-014-9814-x

Brand-Williams, Wendy, Marie-Elisabeth Cuvelier, and C. L. W. T. Berset. "Use of a free radical method to evaluate antioxidant activity." LWT-Food science and Technology 28.1 (1995): 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5

Olive, Peggy L., and Judit P. Banáth. "The comet assay: a method to measure DNA damage in individual cells." Nature protocols 1.1 (2006): 23-29. https://doi.org/10.1038/nprot.2006.5

##submission.downloads##

Опубліковано

2024-05-17