ШЛЯХИ ОПТИМІЗАЦІЇ МЕТАБОЛІЗМУ ШТАМУ CORYNEBACTERIUM GLUTAMICUM ATCC 21831 ДЛЯ ВИРОБНИЦТВА L-АРГІНІНУ
Ключові слова:
amino acid, L-arginine, Corynebacterium glutamicum ATCC 21831 AR6, metabolic pathwayАнотація
We describe the improvement of the strain Corynebacterium glutamicum ATCC 21831 for the production of L-arginine. First, its tolerance to L-arginine analogs was increased by random mutagenesis. Then, systemic metabolic engineering was applied by removing regulatory repressors, optimizing NADPH levels, disrupting L-glutamate export, and tuning biosynthetic reactions to increase L-arginine production.
Посилання
Kurhaluk N. The Effectiveness of L-arginine in Clinical Conditions Associated with Hypoxia. International Journal of Molecular Sciences. 2023. Vol. 24, no. 9. P. 8205. URL: https://doi.org/10.3390/ijms24098205.
Metabolic engineering of Corynebacterium glutamicum for L-arginine production / S. H. Park et al. Nature Communications. 2014. Vol. 5, no. 1. URL: https://doi.org/10.1038/ncomms5618.
Interaction of Transcriptional Repressor ArgR with Transcriptional Regulator FarR at the argB Promoter Region in Corynebacterium glutamicum / S. Y. Lee et al. Applied and Environmental Microbiology. 2010. Vol. 77, no. 3. P. 711–718. URL: https://doi.org/10.1128/aem.01610-10.
Mutations of the Corynebacterium glutamicum NCgl1221 Gene, Encoding a Mechanosensitive Channel Homolog, Induce L-Glutamic Acid Production / J. Nakamura et al. Applied and Environmental Microbiology. 2007. Vol. 73, no. 14. P. 4491–4498. URL: https://doi.org/10.1128/aem.02446-06.
