АНАЛІЗ ЕФЕКТИВНОСТІ ВИКОРИСТАННЯ РЕКОМБІНАНТНИХ БАКТЕРІАЛЬНИХ ШТАМІВ ДЛЯ ПІДВИЩЕННЯ ПРОДУКТИВНОСТІ ПУЛУЛАНАЗИ
Ключові слова:
pululanase, recombinant, enzyme production, cultivationАнотація
Bacillus subtilis WB800, WB600, BS001 and Escherichia coli BL 21 have shown promise in enhancing pululanase productivity. Introduction of pululanase-encoding genes into their genomes significantly increased enzyme activity. Optimization of cultivation conditions further boosted activity, demonstrating potential for industrial-scale production and diverse biotechnological applications.
Посилання
Wang Y., Chen S., Zhao X., Zhang Y., Wang X., Nie Y., Xu Y. Enhancement of the production of Bacillus naganoensis pullulanase in recombinant Bacillus subtilis by integrative expression. Protein Expression and Purification. 2019. doi:10.1016/j.pep.2019.03.006.
Chi L., Wei J., Hou J., Wang J., Hu X., He P., Wei T. Optimizing the DO‐stat protocol for enhanced production of thermostable pullulanase in Escherichia coli by using oxygen control strategies. Journal of Food Biochemistry. 2020. doi:10.1111/jfbc.13173.
Meng F., Zhu X., Zhao H., Lu F., Lu Y., Lu Z. Improve Production of Pullulanase of Bacillus subtilis in Batch and Fed-Batch Cultures. Applied Biochemistry and Biotechnology. 2020, 193(1): Р. 296–306. doi:10.1007/s12010-020-03419-2.
