Біотехнологія XXI століття

ДОСЛІДЖЕННЯ БІОЧОРНИЛ З ХІТОЗАНУ ДЛЯ БІОДРУКУ

В. А. Ахмедова, С. А. Гайдук, О. І. Голембіовська — пт, 17 тра 2024 00:00:00 +0300

In this study, a technique for manufacturing a chitosan gel and selection of characteristics for multilayer printing of a chitosan scaffold on a bioprinter and was determinated of rheological characteristics are proposed. The characteristics of bioprinting and a detailed description of the improvement of the hydrogel production process are given.

РОЛЬ МОЛЕКУЛЯРНОЇ БІОТЕХНОЛОГІЇ В РОЗРОБЦІ ПЕРСОНАЛІЗОВАНОЇ МЕДИЦИНИ: ВІД ГЕНЕТИЧНИХ ТЕСТІВ ДО ІНДИВІДУАЛІЗОВАНОЇ ТЕРАПІЇ

В. А. Ахмедова — пт, 17 тра 2024 00:00:00 +0300

The article examines the important role of molecular biotechnology in the development of personalized medicine, ranging from the use of genetic tests to individualized therapy. Special attention is paid to the methods of genomic data analysis and their influence on the appointment of treatment, which makes it possible to effectively treat diseases in a unique way for each patient.

ЗАГАЛЬНЕ ТА ОСОБЛИВОСТІ БІОТЕХНОЛОГІЙ ХАРЧОВИХ КИСЛОТ

М. І. Бабак, Г. С. Литвинов — пт, 17 тра 2024 00:00:00 +0300

This article provides a brief overview of the most common food acids, including acetic, citric, malic, lactic and fumaric acids, including information on their use in the food industry. It also provides information on modern methods of production and the world's largest producers, as well as a brief description of their role in the human body.

АНАЛІЗ ЕФЕКТИВНОСТІ ВИКОРИСТАННЯ РЕКОМБІНАНТНИХ БАКТЕРІАЛЬНИХ ШТАМІВ ДЛЯ ПІДВИЩЕННЯ ПРОДУКТИВНОСТІ ПУЛУЛАНАЗИ

О. М. Бабко — пт, 17 тра 2024 00:00:00 +0300

Bacillus subtilis WB800, WB600, BS001 and Escherichia coli BL 21 have shown promise in enhancing pululanase productivity. Introduction of pululanase-encoding genes into their genomes significantly increased enzyme activity. Optimization of cultivation conditions further boosted activity, demonstrating potential for industrial-scale production and diverse biotechnological applications.

ПОРІВНЯЛЬНИЙ АНАЛІЗ ПРОДУКТИВНОСТІ ШТАМІВ SACCHAROPOLYSPORA ERYTHRAEA У ВИРОБНИЦТВІ ЕРИТРОМІЦИНУ

О. М. Бабко — пт, 17 тра 2024 00:00:00 +0300

This article explores the selection of a high-productivity strain of Saccharopolyspora erythraea for erythromycin production. Comparative analysis revealed that strain 52 exhibited the highest productivity, making it a promising candidate for enhancing erythromycin production efficiency and meeting market demands.

РЕГУЛЯЦІЯ АНТИАДГЕЗИВНОЇ АКТИВНОСТІ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН ACINETOBACTER CALCOACETICUS IMВ B-7241 ЗА НАЯВНОСТІ КОНКУРЕНТНИХ ГРАМНЕГАТИВНИХ БАКТЕРІЙ

Д. О. Благодир — пт, 17 тра 2024 00:00:00 +0300

The article is devoted to the study of the influence of competitive gram-negative bacteria as a factor in the regulation of the anti-adhesive activity of surfactants synthesized by Аcinetobacter calcoaceticus ІMV B-7241. The degree of bacterial test cultures adhesion on abiotic surfaces treated with solutions of surfactants synthesized in the presence of an inducer was 8-19% lower than under the action of surface-active substances obtained without an inducer.

ВИЗНАЧЕННЯ ВМІСТУ ФЛАВОНОЇДІВ ТА АНТИОКСИДАНТНОЇ АКТИВНОСТІ У «БОРОДАТИХ» КОРЕНЯХ РОСЛИН CICHORIUM INTYBUS L.

Т. А. Богданович, Н. А. Матвєєва — пт, 17 тра 2024 00:00:00 +0300

This paper is devoted to the screening of nine chicory hairy root lines by their specific flavonoid content and antioxidant activity. In all studied lines values of both parameters were higher than in the control (non-transformed roots). The results of the screening allowed to select lines with the highest values of flavonoid content and antioxidant activity for further research.

МЕТА-АНАЛІЗ НАУКОВИХ І КЛІНІЧНИХ ДОСЛІДЖЕНЬ HEALTHSPAN-ANTI-AGEING ЕФЕКТІВ БІОТЕХНОЛОГІЧНО ОТРИМУВАНИХ АМІНОКИСЛОТ ТА ЇХ ДЕРИВАТІВ

Б. О. Боярський, А. О. Бондар, Г. С. Литвинов — пт, 17 тра 2024 00:00:00 +0300

This article provides a brief review of literature data on the most common amino acids and their derivatives that have an antiaging effect on the human body. It also contains information about modern biotechnological methods of production of the amino acids in question, their therapeutic effect and the world's largest producers.

THE INFLUENCE OF CULTIVATION CONDITIONS ON THE GROWTH OF MYCOPLASMA HOMINIS

L. V. Vlasenko, S. М. Halytska, I. V. Nikolaienko — пт, 17 тра 2024 00:00:00 +0300

This study is dedicated to the comparative analysis of the efficiency of cultivating Mycoplasma hominis using surface and submerged methods and assessing the impact of growth characteristics of horse serum on bacterial biomass production during surface cultivation.

ВПЛИВ ТРИПТОФАНУ ТА ЕРИТРИТОЛУ НА АНТИМІКРОБНУ ДІЮ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН RHODOCOCCUS ERYTHROPOLIS IMB Aс-5017

А. М. Воробей, Т. П. Пирог, Т. А. Шевчук — пт, 17 тра 2024 00:00:00 +0300

This article focuses on the study of the phytohormones precursors influence on the biological activity of Rhodococcus erythropolis IMV Ac-5017 surfactants against phytopathogenic bacteria. It was found that in their presence in the cultivation medium of IMV Ac-5017 strain the obtained surfactants had 2-4 times higher antimicrobial activity compared to the preparations without precursors.

ПРОБЛЕМИ ТА ПЕРСПЕКТИВИ МОЛЕКУЛЯРНО-ГЕНЕТИЧНОЇ ДІАГНОСТИКИ ГЕМОФІЛІЇ А

Д. О. Воробйов, Н. В. Грищенко — пт, 17 тра 2024 00:00:00 +0300

Haemophilia A (HA) is a severe hereditary disease. HA affects males while females are asymptomatic carriers. DNA diagnostics is the most informative method of HA diagnostics. The aim is the selection of the diagnostic strategy and a research group creation. The most justified strategy for HA diagnostics was selected. An experimental group of patients and family members was created.

ВПЛИВ НАНОГІДРОКСИАПАТИТІВ НА РОСЛИНИ

І. Є. Галицька, І. І. Гринюк — пт, 17 тра 2024 00:00:00 +0300

The negative impact of synthetic chemicals on the environment and human health can be reduced by adopting the latest technological approaches. This can be achieved by using nanohydroxyapatites as nanofertilizer and nanopriming are considered. Nanoparticles are effective because of their small size and unique physio-chemical properties, which make them an ideal seed priming agent.

ІМУНОЛОГІЧНЕ ПІДҐРУНТЯ ЕФЕКТИВНОСТІ ПРОБІОТИЧНИХ МІКРООРГАНІЗМІВ

Д. С. Голубчик, О. М. Дуган — пт, 17 тра 2024 00:00:00 +0300

These theses are devoted to the determination of the most prominent probiotic supplements on the market (based on experts’ opinions) and elucidating the immunological basis for their widespread use. The most important factors influencing probiotics effectiveness include epithelial barrier enhancement, competition with different pathogens, the ability to impact cell maturation and activity and increase the level of cytokines and immunoglobulins production.

EVALUATION OF THE POTENTIAL OF YEAST STRAINS FOR USE IN SOURDOUGHS

Y. R. Honchar, O. V. Naumenko, V. M. Chizh, L. V. Marynchenko — пт, 17 тра 2024 00:00:00 +0300

This study assessed four pre-isolated Saccharomyces cerevisiae strains for sourdough suitability, focusing on fermentative capabilities, osmo- and thermotolerance. Results revealed robust fermentation of diverse sugars and resilience under adverse conditions, indicating promising prospects for efficient and consistent sourdough production.

РОЗПОДІЛ ПЕРОРАЛЬНО ВВЕДЕНИХ НАНОЧАСТИНОК ОКСИДУ ЗАЛІЗА В ОРГАНАХ ТА ТКАНИНАХ РИБ DANIO RERIO

М. О. Громнадська, Ю. В. Лебединська — пт, 17 тра 2024 00:00:00 +0300

This article investigated the distribution of orally administered iron oxide nanoparticles in the organs and tissues of Danio rerio fish through the Perl's reaction. It was revealed that orally administered iron oxide magnetic nanoparticles accumulate mainly in brain, liver, kidney, and heart of Danio rerio fish in which the presence of biogenic magnetic nanoparticles was shown.

ОЦІНКА ВПЛИВУ ОВОЧЕВИХ НАПОВНЮВАЧІВ У СКЛАДІ ПОЖИВНОГО СЕРЕДОВИЩА ДЛЯ КУЛЬТИВУВАННЯ МОЛОЧНОКИСЛИХ БАКТЕРІЙ

Є. І. Губар, І. М. Корнієнко — пт, 17 тра 2024 00:00:00 +0300

The effect of adding vegetable supplements to MRS nutrient media and meat peptone broth on the titer of lactic acid bacteria (LAB) was investigated. As a source of LAB, "FIT-YOGURT" sourdough of the VIVO brand was used. It was established that the addition of carrots, beets and cabbage regardless of the chosen concentration (3%, 5%, 7%, 10%) increases the titer of LAB.

ДОСЛІДЖЕННЯ АКТИВНОСТІ ЕНДО-1,4-β-ГЛЮКАНАЗИ У ШТАМУ ДЕРЕВОРУЙНУЮЧОГО БАЗИДІОМІЦЕТУ SCHIZOPHYLLUM COMMUNE

Ю. О. Гузенко, В. М. Ліновицька — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the study of the cellulolytic activity of the enzyme endo-1-4-b-glucanase in a straiєn of wood-destroying basidiomycete Schizophyllum commune. It was established that the maximum value of endo-1,4-betta-glucanase activity in the S. commune 1770 strain was 0.213±0.012 IU/cm³ on a synthetic medium with carboxymethylcellulose (6 days of cultivation).

ДОСЛІДЖЕННЯ СТУПЕНЮ ПРОТЕОЛІЗУ КОНЦЕНТРАТУ БІЛКОВОГО ІЗ МОЛОЧНОЇ СИРОВАТКИ ТА ІЗОЛЬОВАНОГО СОЄВОГО БІЛКА В ЗАЛЕЖНОСТІ ВІД МАСОВОЇ ЧАСТКИ ФЕРМНЕТНИХ ПРЕПАРАТІВ

Г. В. Декуша, М. М. Козак — пт, 17 тра 2024 00:00:00 +0300

The article is devoted to the study of proteolysis of whey protein concentrate (WPC) and isolated soy protein (ISP). The results of experimental studies of the process of enzymatic hydrolysis of ISB and WPC by enzyme preparations "Protamex", "Protease C" and "ORBAproteo P-1200", which are currently available on the domestic market, showed a high specificity to proteins.

ШЛЯХИ ОПТИМІЗАЦІЇ МЕТАБОЛІЗМУ ШТАМУ CORYNEBACTERIUM GLUTAMICUM ATCC 21831 ДЛЯ ВИРОБНИЦТВА L-АРГІНІНУ

Є. О. Журомський, М. М. Непомняща, Ю. П. Горго — пт, 17 тра 2024 00:00:00 +0300

We describe the improvement of the strain Corynebacterium glutamicum ATCC 21831 for the production of L-arginine. First, its tolerance to L-arginine analogs was increased by random mutagenesis. Then, systemic metabolic engineering was applied by removing regulatory repressors, optimizing NADPH levels, disrupting L-glutamate export, and tuning biosynthetic reactions to increase L-arginine production.

КУЛЬТИВУВАННЯ PLEUROTUS OSTREATUS НА ПЕКТИНІ

П. Р. Зубик, І. Р. Клечак — пт, 17 тра 2024 00:00:00 +0300

The study examined the influence of different pH levels on the growth of two Pleurotus ostreatus strains when cultivated solely on pectin as a source of nutrients. It was observed that the P. ostreatus 330 strain was found to be more suitable for cultivation on pectin-containing media compared to the P. ostreatus 228 strain, indicating its potential for the effective utilization of industrial lignocellulosic waste.

АНТАГОНІСТИЧНА АКТИВНІСТЬ ШТАМІВ TRICHODERMA ЩОДО ТОКСИГЕННИХ ПРЕДСТАВНИКІВ ASPERGILLUS SULPHUREUS ТА A. OCHRACEUS

П. П. Каганюк, Я. І. Савчук — пт, 17 тра 2024 00:00:00 +0300

The study is devoted to the evaluation of the antagonistic activity of Trichoderma strains against toxigenic strains of Aspergillus sulphureus and A. ochraceus. It was shown that strains of T. atroviride 3123 and T. virens 66№3 showed the highest levels of antifungal activity against Aspergillus test cultures by 53% of inhibition rates.

АНТИМІКРОБНІ ВЛАСТИВОСТІ БАЗИДІЄВИХ ГРИБІВ

Ю. А. Калюжний — пт, 17 тра 2024 00:00:00 +0300

Macromycetes Laetiporus sulphureus are widely used in medicine due to their antimicrobial potential. The present work is devoted to the analysis of scientific articles on the topic of the antibiotic activity of the alcohol extract of L. sulphureus and its ability to inhibit the growth of biofilms. The analysed results demonstrate that the fungal metabolites have a broad antimicrobial spectrum and high efficiency in the range of non-toxic concentrations (7-10 mg/ml).

COMPARATIVE ANALYSIS OF METHODS FOR SEROLOGICAL DIAGNOSIS OF MYCOPLASMOSIS CAUSED BY MYCOPLASMA HOMINIS

Y. Kyrylenko, V. Klochko — пт, 17 тра 2024 00:00:00 +0300

This article presents serological diagnostic methods for detecting antibodies to Mycoplasma hominis and identifies the advantages and disadvantages of the most promising of them. Also, the classes of immunoglobulins that are formed in the human body as a reaction to inflammatory processes caused by M. hominis are indicated.

ОСОБЛИВОСТІ МОРФОЛОГІЇ ШТАМІВ FOMITOPSIS BETULINA НА АГАРИЗОВАНИХ ПОЖИВНИХ СЕРЕДОВИЩАХ

Т. О. Кізіцька, Т. А. Круподьорова, В. В. Ратушняк , В. Ю. Барштейн — пт, 17 тра 2024 00:00:00 +0300

The aim of this paper is to observe and discuss Fomitopsis betulina strains' features during its cultivation on different agarized media. Twenty-two strains of F. betulina were processed during investigation. Morphological differences of mycelium, character of pigmentation, formation of primordia were recognized and noted.

CULTIVATION OF TRAMETES SP. ON AGARIFIED PECTIN-CONTAINING MEDIA

I. Klechak, P. Zubyk — пт, 17 тра 2024 00:00:00 +0300

This study examines the growth of Trametes strains on nutrient media with and without pectin, revealing significant differences. While both strains grew rapidly on standard media, T. hirsutus 1569 exhibited slower growth on pectin-enriched media compared to T. versicolor 1689. These findings suggest T. versicolor 1689 as a promising candidate for further investigation into its potential for pectinase synthesis.

BIOSYNTHESIS OF SILVER NANOPARTICLES AFTER CULTIVATING MUTANT YEAST ON CULTURE MEDIA OF DIFFERENT COMPOSITIONS

R. V. Koval, O. I. Skrotska — пт, 17 тра 2024 00:00:00 +0300

Current work is devoted to the determination of the optimal parameters of the biosynthesis of silver nanoparticles using a mutant strain of Saccharomyces cerevisiae yeast. Optimal parameters of nanoparticles biosynthesis were established: cultivation of yeast on YPD 1 medium, the use of cell-free aqueous extract of mutant yeast, 1.5 mM AgNO₃, 45℃.

PLEUROTUS ERYNGII ЯК ПЕРСПЕКТИВНИЙ ПРОДУЦЕНТ БІОЛОГІЧНО АКТИВНИХ СПОЛУК ЛІКУВАЛЬНО-ПРОФІЛАКТИЧНОЇ ДІЇ

А. О. Корочева — пт, 17 тра 2024 00:00:00 +0300

This work is dedicated to the analysis of publications describing biological activity of P. eryngii metabolites and possibility of its cultivation by submerged method. The results of analysis confirm biological activity of P. eryngii metabolites. P. eryngii grows on available substrates in submerged culture, showing potential as a producer of therapeutic and prophylactic biotechnological products.

НАКОПИЧЕННЯ ЕКЗОПОЛІСАХАРИДІВ ПРОМИСЛОВИМИ ШТАМАМИ PLEUROTUS OSTREATUS

О. В. Кузнецова, К. М. Власенко, Л. М. Павленко — пт, 17 тра 2024 00:00:00 +0300

The scientific paper presents studies on the accumulation of exopolysaccharides by industrial strains of Pleurotus ostreatus (IBK-1535, IBK-551, K-12) during surface cultivation on a glucose-ammonium liquid nutrient medium. It was determined that strain IBK-551 had the best indicators – 11.0 g/l. Strains IBK-1535 and K-12 - 4.3 and 4.7 g/l, respectively.

БІОСИНТЕТИЧНА ЗДАТНІСТЬ EREMOTHECIUM ASHBYI ПРИ КУЛЬТИВУВАННІ НА ВІДХОДАХ ОЛІЙНО-ЖИРОВОЇ ПРОМИСЛОВОСТІ

П. С. Лазарець, В. Ю. Поліщук — пт, 17 тра 2024 00:00:00 +0300

The article is devoted to the study of biosynthetic properties when cultivated on oil and fat industry wastes. The meal and cake were used as cheap raw materials for the cultivation of Eremothecium ashbyi. The ability of the producer to synthesize riboflavin on these wastes of the oil and fat industry was analyzed.

АНТАГОНІСТИЧНІ ВЛАСТИВОСТІ ШТАМУ STREPTOMYCES SP. LG23

Д. В. Лижнюк, Е. М. Гебеш, В. В. Клочко — пт, 17 тра 2024 00:00:00 +0300

In the work, a study of the antagonistic activity of the Streptomyces sp. LG23 strain isolated by us from the soil was carried out. A high activity of the streptomycete against gram-positive bacteria Micrococcus lysodeikticus and Staphylococcus aureus was established; growth inhibition zones were 20 and 18 mm respectively. Moderate activity (zones of 14-16 mm) of the streptomycete was found against the species Bacillus subtilis and Sarcina flava. Presumably, the strain Streptomyces sp. LG23 is a producer of antibiotic compounds active against gram-positive bacteria.

ДОСЛІДЖЕННЯ АКТИВНОСТІ МОНОФЕНОЛМОНООКСИГЕНАЗИ У БАЗИДІЄВОГО ГРИБА GRIFOLA FRONDOSA В ГЛИБИННІЙ КУЛЬТУРІ

Н. С. Литвин, В. М. Ліновицька — пт, 17 тра 2024 00:00:00 +0300

The presented materials are dedicated to the study of the basidiomycete Grifola frondosa in submerged culture. The influence of the composition of the medium was established and the most favorable glucose concentration for the accumulation of mycelial biomass was determined, and the activity of the monophenol monooxygenase enzyme was revealed.

ШТАМИ МОЛОЧНОКИСЛИХ БАКТЕРІЙ ЯК ІНОКУЛЯНТИ ПРИ ФЕРМЕНТАЦІЇ СИЛОСУ З ЖИТА

А. С. Лук’янець, С. Г. Даниленко — пт, 17 тра 2024 00:00:00 +0300

Accordingly, the aim of the study was to create a promising bacterial composition based on strains Lactobacillus buchneri, L. plantarum L. brevis та L. rhamnosus. It was this composition that was evaluated as promising for the creation of a new biopreparation for rye ensiling based on it.

ПРОТЕОЛІТИЧНА АКТИВНІСТЬ ШТАМІВ 320 І 1515 МІКРОМІЦЕТУ TRICHODERMA KONINGII

А. О. Матвієнко, С. О. Сирчін — пт, 17 тра 2024 00:00:00 +0300

Proteases, along with chitinases and laminarinases, are part of the triad of hydrolytic enzymes providing biocontrol potential of the fungi Trichoderma genus, but remain largely unexplored so far. We therefore conducted a comparative evaluation of the potential to produce extracellular proteolytic activity of Trichoderma koningii strains 320 and 1515 were highly active against phytopathogens.

ARTEMISIA VULGARIS “HAIRY” ROOTS AS A SOURCE OF SAFE ANTIOXIDANT COMPOUNDS

пт, 17 тра 2024 00:00:00 +0300

The present study aimed to investigate flavonoid content and antioxidant activity of Artemisia vulgaris “hairy” roots of 3 lines. We also studied the safety of transgenic root extracts by determining their genotoxicity. The total flavonoid content in water extracts of the fresh “hairy” roots reached up to 1.976 ± 0.1 mg/g FW, which is 4.8 times higher than in control plant roots. Extracts of transgenic roots on average showed 1.5 times higher antioxidant activity compared to untransformed roots. The comet assay results indicated the absence of the genotoxic effect of all tested extracts. Therefore, these extracts can be the source of safe antioxidant compounds.

ПРАКТИЧНЕ ВИКОРИСТАННЯ КОНТРОЛЮ РОЗЧИНЕНОГО КИСНЮ ПІД ЧАС КУЛЬТИВУВАННЯ РЕКОМБІНАНТНОГО ШТАМУ ESCHERICHIA COLI ПРИ ВИРОБНИЦТВІ ІНСУЛІНУ ЛЮДИНИ

В. В. Орлов — пт, 17 тра 2024 00:00:00 +0300

The article describes the observation of the behavior of dissolved oxygen during the cultivation of Escherichia coli cells and the possibility of further selection of components for further insulin synthesis depending on the type of dissolved oxygen behavior during each specific cultivation. The practical use of observing the behavior of dissolved oxygen, depending on its nature, in industrial applications, will increase the yield of the target product and simplify the further synthesis process.

ДІЯ СУМІШІ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН RHODOCOCCUS ERYTHROPOLIS IMB Aс-5017 З ЕФІРНИМИ ОЛІЯМИ НА ДВОВИДОВІ БІОПЛІВКИ

А. М. Охмакевич, Є. А. Дон, Л. В. Ключка, Т. П. Пирог — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the destruction degree of dual-species bacterial and yeast biofilms under the influence of a mixture of tea tree or cinnamon essential oil and Rhodococcus erythropolis IMV Ac-5017 surfactants, synthesized in the presence of the yeast inducer. It was found that surfactants synthesized in the presence of Saccharomyces cerevisiae БТМ-1 showed synergism of antibiofilm activity with the tested essential oils.

СТВОРЕННЯ КОНСТРУКЦІЙ ДЛЯ БАГАТОКОПІЙНОЇ ІНТЕГРАЦІЇ ГЕНІВ ІНТЕРЕСУ В ДЕЛЬТА ПОСЛІДОВНОСТІ ГЕНОМУ ДРІЖДЖІВ

О. Г. Підкурганна , Л. Б. Зелена, С. М. Шульга — пт, 17 тра 2024 00:00:00 +0300

Yeasts are attractive cell factories for recombinant protein production. Stable integration and high-copy number of gene interest are essential for strain productivity. Constructions designed in this work are intended for CRISPR-mediated multi-copy integrations into delta-sites of Saccharomyces cerevisiae genome.

ЕФЕКТИВНІСТЬ ВКЛЮЧЕННЯ ПРОБІОТИЧНИХ ПРЕПАРАТІВ В СХЕМУ ЛІКУВАННЯ ДІТЕЙ З ГОСТРИМ ГАСТРОЕНТЕРИТОМ

В. В. Піць, С. О. Соловйов — пт, 17 тра 2024 00:00:00 +0300

In these theses, we investigated the effect of additional probiotic therapy on the number of days of hospitalization for rotavirus infection. The initial data was a set of retrospective data on children with rotavirus infection and their treatment. The distributions of hospitalization days were investigated.

ТЕХНОЛОГІЯ СТВОРЕННЯ НАДПРОДУЦЕНТА ІНТЕРФЕРОНУ ШЛЯХОМ КОНТРОЛЮ LAC-ОПЕРОНА У E. COLI

А. В. Постнікова — пт, 17 тра 2024 00:00:00 +0300

This article explores the diverse roles of interferons, their antiviral and antibacterial effects, as well as the technology that may enable the creation of a high-yield producer strain of E. coli BL21 (DE3). Genetic modification of this microorganism could increase the output of recombinant γ-IFN, offering economically efficient solutions for industrial needs.

МЕТОДИ ОТРИМАННЯ ЕКТРАЦЕЛЮЛЯРНОГО МАТРИКСУ ДЛЯ БІОІНЖЕНЕРІЇ НИРКИ

А. В. Прозор, Т. М. Луценко — пт, 17 тра 2024 00:00:00 +0300

The creation of a kidney organoid begins with the preparation of a three-dimensional substrate for further colonization with cells. The native architecture of the kidney matrix is difficult to create, so it was decided to isolate it from the organ by removing cells. Decellularization can occur through physical, chemical, biological agents, perfusion, or soaking and stirring.

ОТРИМАННЯ TAQ-ПОЛІМЕРАЗИ В КУЛЬТУРІ ESCHERICHIA COLI

Д. К. Прохоренко — пт, 17 тра 2024 00:00:00 +0300

The constructs and methods for analyzing the obtained recombinant protein of Taq polymerase are described in this work. The obtained Taq polymerase, with a mass of 94 kDa and free of DNA nuclease contaminants, exhibited a purity exceeding 90% and an activity level comparable to that of the commercial enzyme sample.

FUSION PROTEINS IN BRAIN DRUG DELIVERY

B. Ruzhytska, O. Holembiovska — пт, 17 тра 2024 00:00:00 +0300

This paper reviews the use of fusion proteins for overcoming the blood-brain barrier in the treatment of central nervous system disorders. Various fusion protein strategies, including monoclonal antibodies and peptide fusion partners, are examined, highlighting their potential in enhancing CNS drug delivery.

ВПЛИВ ЕКСТРАКТІВ ШТАМІВ TRICHODERMA З АНТИБІОТИЧНОЮ АКТИВНІСТЮ НА РІСТ ПРОРОСТКІВ AMARANTHUS HYPOCHONDRIACUS

К. С. Сазонова, Я. І. Савчук — пт, 17 тра 2024 00:00:00 +0300

The effect of extracts of T. pseudokoningii 2926, T. koningii 2768, T. hamatum 3078 strains on the growth of Amaranthus hypochondriacus seedlings was studied. The extracts in dilutions of 1:100 and 1:500 showed a high level of growth stimulating activity. The presence of biologically active metabolites with a stimulating effect on plants in the extracts of all strains of Trichoderma was proved.

ВПЛИВ МЕХАНОЧУТЛИВИХ КАНАЛІВ КАЛЬЦІЮ НА ОНКОГЕНЕЗ

Є. А. Салацький, С. В. Горобець — пт, 17 тра 2024 00:00:00 +0300

Mechanosensitivity of calcium channels affects oncogenesis, which includes both effects on tumor cells, and cells of environment, such as endothelial cells. Low-frequency magnetic field can be applied together with biogenic or artificially administrated magnetic nanoparticles for gating the mechanosensitive calcium ion channels in cancer cells as a novel approach for cancer therapy.

БІОСИНТЕТИЧНА ЗДАТНІСТЬ МОЛОЧНОКИСЛИХ БАКТЕРІЙ НАКОПИЧУВАТИ НАНОЧАСТИНКИ СЕЛЕНУ

Д. А. Синявська, Н. М. Грегірчак — пт, 17 тра 2024 00:00:00 +0300

From 500 million to 1 billion people in the world suffer from Se deficiency. The objective of this work was to select the optimal biological agent capable of accumulating SeNPs. Studies have shown that the bacteria L. delbrueckii ssp. bulgaricus showed the best ability to accumulate Se - 12.05±0.43 mg/ml, which was accompanied by a relative maximum of living cells at Na₂SeO₃ content of 80 mg/ml.

ОЦІНКА ЦИТОТОКСИЧНОСТІ ДЕКАМЕТОКСИНУ НА КЛІТИННУ ЛІНІЮ HEP-2

М. П. Сметюх, С. О. Соловйов, О. П. Трохименко — пт, 17 тра 2024 00:00:00 +0300

CD50 and MTD of decamethoxin, which is widely used in medicine, were determined. The study demonstrated virtually unchanged cytotoxicity of the compound on cells of the HEP-2 line within 72 hours. The toxicity of the compound did not increase with time, which indicates the absence of accumulation and the relative resistance of cells to decamethoxin.

ВИРОБНИЦТВО α-АМІЛАЗИ ЯК ПЕРСПЕКТИВНИЙ ПРОТИБІОПЛІВКОВИЙ АГЕНТ

Я. А. Смірнова, Т. М. Луценко — пт, 17 тра 2024 00:00:00 +0300

In this work, we characterized α-amylase synthesized from bacteria and their prospectives of using it as an anti-biofilm agent. Numerous infectious diseases are connected with microbial biofilms. They are also antibiotic-resistant because of their structure. Enzymes, especially α-amylase produced by Bacillus sp. using submerged fermentation, are effective methods of biofilm disruption due to their biodegradation.

ЗАСТОСУВАННЯ АЛГОРИТМІВ ЕВОЛЮЦІЙНОЇ ОПТИМІЗАЦІЇ ДЛЯ КУЛЬТИВУВАННЯ БАЗИДІЄВИХ МАКРОМІЦЕТІВ

М. Ю. Сокольницький — пт, 17 тра 2024 00:00:00 +0300

This study investigates the application of such algorithms to optimize substrate parameters, treating them as black-box functions affecting fungal growth. By mimicking natural selection processes, evolutionary strategies enhance the search for optimal substrate conditions, surpassing traditional methods like regression analysis or exhaustive search. The research aims to assess the feasibility of evolutionary optimization strategies in biotechnological applications.

ОПТОГЕНЕТИКА. ОПТОГЕНЕТИЧНІ ЗАСОБИ У БІОТЕХНОЛОГІЇ

К. М. Стогній, О. П. Кузь — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to optogenetics as a science combining physics and biology, its application in biotechnology, and the latest research techniques. Various optogenetic tools are observed and the mechanisms of their work, as well as their possible application areas.

ПРОБЛЕМА АГРЕГАЦІЇ БІЛКОВИХ ПРЕПАРАТІВ ПРИ БІОФАРМАЦЕВТИЧНОМУ ВИРОБНИЦТВІ

А. М. Федорко, І. І. Гринюк — пт, 17 тра 2024 00:00:00 +0300

Different mechanisms of protein molecule aggregation were reviewed. Aggregation represents a significant challenge in the field of biopharmaceuticals. We examine the problem of protein molecule aggregation, existing methods for its analysis and control, and strategies for minimizing aggregation at different drug development and manufacturing stages.

ЗЕЛЕНИЙ СИНТЕЗ НАНОЧАСТИНОК СРІБЛА І ОКСИДУ ЦИНКА ТА ОЦІНКА ЇХ АНТИМІКРОБНОЇ АКТИВНОСТІ

пт, 17 тра 2024 00:00:00 +0300

In this study, we report the green synthesis of silver nanoparticles (AgNP) and zinc oxide nanoparticles (ZnОNP) using aqueous extracts of Matricaria chamomilla and Calendula officinalis flowers. High antimicrobial activity of the synthesized nanoparticles against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29213 test microorganisms was shown.

ЕФЕКТ ПІДКИСЛЕННЯ СУСЛА МОЛОЧНОКИСЛИМИ БАКТЕРІЯМИ

А. Д. Хабленко, С. Г. Даниленко, О. І. Яловенко — пт, 17 тра 2024 00:00:00 +0300

The theses are devoted to the study of the growth of a lactic acid bacteria isolate on wort and its acidification at different temperatures. The ability of the isolate to grow and the effect of the acidification of the medium during cultivation at temperatures of 37 °C and 45 °C were analyzed. It was established that the optimal temperature for the growth of the studied isolate and achieving a lower pH value is 37 °С.

MICROBIOLOGICAL ASPECT OF FERMENTATION OF VIBURNUM OPULUS L.

A. D. Khablenko, O. M. Duhan, D. S. Holubchyk — пт, 17 тра 2024 00:00:00 +0300

The theses are devoted to the study of viburnum fermentation process, the analysis of changes in the amount of yeast, the total number of microorganisms and lactic acid bacteria, as well as potential factors of influence on the studied groups of microorganisms. Changes in pH values, the number of microorganisms on different media, and potential adaptive properties of the microbiota were revealed.

МЕДИКО-БІОЛОГІЧНЕ ОБҐРУНТУВАННЯ ВИКОРИСТАННЯ ІНКАПСУЛЯЦІЇ СТОВБУРОВИХ КЛІТИН В ГІДРОГЕЛЕВІ КОНСТРУКЦІЇ

Д. М. Шикета, Н. В. Бертош, В. В. Мотроненко — пт, 17 тра 2024 00:00:00 +0300

This article promotes stem cell encapsulation in hydrogel scaffolds, addressing drug delivery limits. Hydrogels control drug release, shield stem cells, and enhance biocompatibility. Encapsulated cells promise clinical use in tissue regeneration.

СТВОРЕННЯ НАДПРОДУЦЕНТІВ РИБОФЛАВІНУ ШЛЯХОМ РЕГУЛЯЦІЇ АКТИВНОСТІ ІЗОЦИТРАТЛІАЗИ EREMOTHECIUM GOSSYPII

З. С. Шопова, В. Ю. Поліщук — пт, 17 тра 2024 00:00:00 +0300

Eremothecium gossypii, a filamentous hemiascomycete, is a natural overproducer of riboflavin, which protects its spores from UV radiation. Riboflavin acts as an antioxidant and a precursor for flavocoenzymes, crucial for eukaryotic growth and metabolism. Optimizing its cultivation and selecting robust strains through mutagenesis and antimetabolite influence are vital for enhancing productivity.

АНАЛІЗ КЛІНІЧНОЇ ЕФЕКТИВНОСТІ БІОТЕРАПІЇ ІЗ ЗАСТОСУВАННЯМ ПРЕПАРАТУ СУБАЛІН НА ОСНОВІ РІЗНИХ ШТАМІВ BACILLUS SUBTILIS

Ю. А. Яковенко, О. І. Яловенко — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the use of the probiotic drug Subalin within biotherapy in combination with traditional treatment of diseases of different etiology for people of different ages. The clinical effectiveness of industrial strains Bacillus subtilis - UKM V-5020 and 2335/105, as well as the potential of strains still at the stage of clinical research in the prevention and treatment of diseases associated with the vital activity of the intestinal microbiome are considered.

THE EVALUATION AND MANAGEMENT OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA)

V. V. Klochko, U. O. Karpenko — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to analysis of relevant information about methicillin-resistant Staphylococcus aureus, infections that it causes and available detection and treatment. The main danger that MRSA presents is due to its antibiotic resistance, high virulence and contact transmission route. Available treatment of MRSA associated infections consists of antibiotics, like vancomycin and daptomycin. New treatment and prevention methods are in development.

ВПРОВАДЖЕННЯ МЕМБРАННИХ УСТАНОВОК ОЧИЩЕННЯ БІОГАЗУ ДО БІОМЕТАНУ НА БАЗІ ІСНУЮЧИХ БІОГАЗОВИХ ЗАВОДІВ В УКРАЇНІ

М. В. Козловець — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the analysis of current information about the biogas and biomethane market in Ukraine. The main current principles of biogas and biomethane production are presented, the implementation of the first biomethane plant in Ukraine, experience and immediate plans for the development of the biogas industry are described.

ПОШУК ПЕРСПЕКТИВНИХ ВИДІВ ДРІЖДЖІВ, ЗДАТНИХ ЗБРОДЖУВАТИ КСИЛОЗУ, ДЛЯ МАСШТАБОВАНОГО ВИРОБНИЦТВА БІОЕТАНОЛУ

Д. В. Антоненко — пт, 17 тра 2024 00:00:00 +0300

This review explores natural xylose–fermenting yeast strains, such as Spathaspora passalidarum, Ogataea polymorpha, Pachysolen tannophilus, and Scheffersomyces stipitis, highlighting their potential for industrial bioethanol production, noting each yeast species’ distinct advantages and limitations.

ЗАСТОСУВАННЯ БАКТЕРІЙ РОДУ PSEUDOMONAS, ЯК БІОЛОГІЧНОГО АГЕНТУ В БІОРЕМЕДІАЦІЇ ҐРУНТІВ ВІД ПРОМИСЛОВИХ ВИДІВ ЗАБРУДНЕННЯ

А. В. Бондар, А. І. Бондаренко, Р. В. Музичко — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the methods of bioremediation of soils from heavy metal contamination, oil refinery waste, and substances with antibiotic effects. The article presents the methods of soil bioremediation using prokaryotic microorganisms of the genus Pseudomonas as biological agents for the decomposition and transformation of polluting organic and inorganic substances.

БІОІНФОРМАЦІЙНИЙ АНАЛІЗ ГЕНІВ НАБУТОЇ РЕЗИСТЕНТНОСТІ У ЗБУДНИКІВ ТУБЕРКУЛЬОЗУ (MYCOBACTERIUM TUBERCULOSIS)

Т. О. Власюк, В. В. Мотроненко, Н. В. Бертош — пт, 17 тра 2024 00:00:00 +0300

Drug resistance to existing drugs threatens recent advances in the treatment of drug-resistant tuberculosis, and adequate resistance testing for these drugs is virtually unavailable. Therefore, thorough analysis of the causative agents of tuberculosis plays a key role in effectively controlling this disease. With bioinformatics technologies, a new era in the diagnosis and fight against tuberculosis has become possible.

УТИЛІЗАЦІЯ ПОЛІЕТИЛЕНУ ЗА ДОПОМОГОЮ ГРИБІВ

В. В. Головач — пт, 17 тра 2024 00:00:00 +0300

An analysis of literature data on the use of fungi as an effective means of bioremediation was conducted. The research results indicate that fungi show promise in decomposing plastic and polyethylene. Experimental data demonstrate a significant reduction in substrate mass and the fungi’s ability to biodegrade plastic, opening new prospects for the ecological disposal of waste.

РОЛЬ АСКОСПОРОГЕНЕЗУ ТА ІНШИХ ФАКТОРІВ У РЕГУЛЯЦІЇ НАКОПИЧЕННЯ ЛІПІДІВ У ДРІЖДЖАХ DEBARYOMYCES ETCHELLSII

Є. К. Гоман — пт, 17 тра 2024 00:00:00 +0300

The article investigates and analyzes the influence of the ascosporogenesis phase on biochemical processes related to the synthesis and accumulation of lipids in the yeast Debaryomyces etchellsii. Also, these yeast were analyzed for their biotechnological potential in lipid accumulation and through a literature review, the factors affecting the synthesis of lipids by this type of yeast were studied. This research is useful for the development of biodiesel based on microbial lipids.

ВИЗНАЧЕННЯ ОПТИМАЛЬНИХ ПАРАМЕТРІВ ОЧИЩЕННЯ СТІЧНИХ ВОД ШКІРЯНОГО ЗАВОДУ ВІД СПОЛУК АЗОТУ ЗА ВИКОРИСТАННЯ КОМП’ЮТЕРНИХ МОДЕЛЕЙ

А. О. Гриневич, Л. А. Саблій, О. М. Бунчак — пт, 17 тра 2024 00:00:00 +0300

Study aims to optimize tannery wastewater treatment, focusing on nitrogen compound removal via computer modeling. Optimal parameters include nitrate removal 400% and air consumption 20,800 m³/day, potentially saving 50% energy. Expected outlet indicators: ammonium nitrogen – 0.1 mg/dm³ (inlet – 265.5 mg/dm³), nitrites – 0.01 mg/dm³, nitrates – 10.0 mg/dm³, meeting discharge regulations into the Gerinya River, Dniester basin.

АНАЛІЗ ДИФЕРЕНЦІЙНО ЕКСПРЕСОВАНИХ ГЕНІВ І БІОЛОГІЧНИХ ПРОЦЕCІВ У ПЛАЦЕНТІ ЛЮДИНИ МІЖ ПЕРШИМ І ДРУГИМ ТРИМЕСТРОМ ФІЗІОЛОГІЧНОЇ ВАГІТНОСТІ

Р. І. Джікірба, М. Ю. Оболенська — пт, 17 тра 2024 00:00:00 +0300

In this study, a differential expression analysis on microarray was performed to compare the gene expression of 9 samples during the second trimester to 13 samples during the first trimester of normal pregnancy. These results were used to perform gene-set enrichment analysis, the results of which demonstrated 260 enriched biological processes. Both the top 3 activated and inhibited biological processes were illustrated using cnetplot.

PHYLOGENETIC ANALYSIS OF INFLUENZA VIRUS TYPE A H1N1 NEURAMINIDASE GENE ISOLATED FROM HUMAN

L. B. Zelena, D. D. Parchevska, N. V. Tkachuk, S. D. Zahorodnia — пт, 17 тра 2024 00:00:00 +0300

The research was devoted to the study of influenza virus H1N1 neuraminidase gene genetic variability between isolates of various geographic localization and year of collection. The analysis of nucleotide sequence variability and phylogenetic analysis were performed using data from NCBI's Influenza Virus Resource and MEGA 10. The obtained results revealed clear differentiation between isolates of 2009 and the earlier collected ones. The level of genetic polymorphism was low among the isolates of 2009 and 98,6% of nucleotide sites were conservative.

АНАЛІЗ ЕФЕКТИВНОСТІ ВИКОРИСТАННЯ ВІДОМИХ МЕТОДІВ ОЧИЩЕННЯ СТІЧНИХ ВОД ВІД АНТИБІОТИКІВ

Л. С. Кіка, Л. А. Саблій — пт, 17 тра 2024 00:00:00 +0300

The chemical and physical-chemical methods оf wastewater treatment are limited due to high energy consumption and low efficiency. Aerobic biological treatment is also not always effective due to the high persistence of pharmaceutical substances. However, the use of certain plants (Lemna aoukikusa, Lemna minor, Spirodela polyrhiza, Lemna aequinoctialis, Chrysopogon zizanioides) demonstrates the 70 percent or more removal of various types of antibiotics.

ВПЛИВ КОМБІНАЦІЇ ДЖЕРЕЛА ОСВІТЛЕННЯ ТА КОНЦЕНТРАЦІЇ Fe2+ НА РІСТ МІКРОВОДОРОСТЕЙ CHLORELLA VULGARIS

С. О. Ковальова — пт, 17 тра 2024 00:00:00 +0300

This study investigates the combined effects of different light sources and iron concentrations on the growth of Chlorella vulgaris microalgae. Results show significant growth variations under varying experimental conditions. Optimal growth was observed under natural sunlight and red-blue LED light, supplemented with 20 mg/L iron (Fe²⁺). Conversely, white LED light showed a reverse trend.

АНАЛІЗ ЕФЕКТИВНОСТІ ТЕХНОЛОГІЙ ОБРОБКИ БІОМАСИ МІКРОВОДОРОСТЕЙ ДЛЯ ОТРИМАННЯ БІОПАЛИВНИХ СПИРТІВ

Н. В. Козачок, Л. С. Зубченко, Є. О. Журомський — пт, 17 тра 2024 00:00:00 +0300

This article examines the diverse methodologies for preparing microalgae biomass for bioethanol production, delving into the comparative effectiveness of various pre-treatment strategies. It discusses the impact of physical, chemical, and enzymatic processes on enhancing the availability of fermentable sugars necessary for ethanol fermentation, highlighting the potential of microalgae as a sustainable biofuel source.

ДОСЛІДЖЕННЯ СУЧАСНИХ ПАКУВАЛЬНИХ МАТЕРІАЛІВ ЗДАТНИХ ДО БІОЛОГІЧНОГО РОЗКЛАДАННЯ

О. А. Корнелюк, М. Ю. Козар — пт, 17 тра 2024 00:00:00 +0300

The article investigates the biodegradation of polylactic acid (PLA) under vermicomposting conditions. Microscopic analysis revealed a low level of decomposition, which may be due to synthetic fillers. Further research is suggested to optimize biodegradation and improve the quality of PLA.

THE EXPERIMENTAL INVESTIGATIONS OF THE PROCESS OF DRYING FUEL CHIPS FROM WOOD WASTE IN A FIXED LAYER

A. Liashenko — пт, 17 тра 2024 00:00:00 +0300

In Ukraine, wood production remains one of the priority sectors of the national economy. The total area of forests is 10.4 million hectares [1-3]. The advantages of biomass as a fuel are its renewable nature, low ash content, low emissions, and the preservation of the balance of carbon dioxide in the atmosphere [4]. Energy products of biomass processing can be used in common energy installations, for example, as a traditional fuel.

БІОРЕМЕДІАЦІЯ ҐРУНТІВ, ЗАБРУДНЕНИХ РАДІОАКТИВНИМИ ІЗОТОПАМИ

О. В. Неділько, А. Н. Зеленюк, В. С. Жукова — пт, 17 тра 2024 00:00:00 +0300

This article describes methods of bioremediation of soils contaminated with radioactive isotopes because of human activities such as nuclear power. Soils are particularly sensitive to the accumulation of radionuclides and their subsequent migration to other territories and other living organisms. Therefore, their remediation is necessary to prevent further spread of contamination and reduce its impact.

УМОВИ ПРОВЕДЕННЯ МОНІТОРИНГУ РЕАКЦІЇ МЕТАХРОМАЗІЇ ВОЛЮТИНОВИХ ГРАНУЛ ДРІЖДЖІВ ТА ВАРІАЦІЙ ГЕОМАГНІТНОГО ПОЛЯ

М. М. Опаленик, Ю. П. Горго, І. О. Грецький, О. М. Громозова — пт, 17 тра 2024 00:00:00 +0300

The conditions for monitoring the color changes of the metachromatic reaction of volutinous granules of the yeast Saccharomyces cerevisiae and the values of the geomagnetic field intensity at frequencies below 1 Hz for further determination of the correlation coefficients between them, at which significant changes in the reaction are noted.

ВИБІР СЕЛЕКТИВНОГО СЕРЕДОВИЩА ДЛЯ ВІДБОРУ ТРАНСГЕННИХ РОСЛИН ТЮТЮНУ, ОТРИМАНИХ В РЕЗУЛЬТАТІ ОПОСЕРЕДКОВАНОЇ АГРОБАКТЕРІЯМИ ТРАНСФОРМАЦІЇ

О. А. Осипенко, М. О. Банникова, І. О. Нітовська — пт, 17 тра 2024 00:00:00 +0300

Substituting phosphates with phosphites prevents higher plants' regeneration. The ptxD gene allows phosphite use and serves as a negative selection marker. Various MSR-based media with differing phosphite-phosphate ratios were tested for transformant selection. Optimal for selection of Petite Havana tobacco transformants will be a medium comprising 50% phosphates and 50% phosphites.

ГЕНЕТИЧНА МОДИФІКАЦІЯ РОСЛИН ДЛЯ ЗМЕНШЕННЯ ВИКОРИСТАННЯ ФОСФАТІВ: МОЖЛИВОСТІ ТА ВИКЛИКИ

О. А. Осипенко, Л. В. Маринченко, М. О. Банникова, І. О. Нітовська — пт, 17 тра 2024 00:00:00 +0300

This article discusses biotech solutions to overcome phosphorus deficiency in soil, a key challenge in agriculture. Strategies involve boosting phosphorus uptake in plants through genetic tweaks and reclaiming phosphorus from waste. Plants modified to use phosphites offer a promising way to cut reliance on conventional fertilizers, ensuring better yields, quality, and eco-sustainability.

ПОРІВНЯЛЬНИЙ АНАЛІЗ МЕТОДІВ КОМП'ЮТЕРНОГО МОДЕЛЮВАННЯ ТРАНСКРИПЦІЙНОГО ФАКТОРА СТРЕСОСТІЙКОСТІ WRKY2 У TRITICUM AESTIVUM

А. П. Петровський, І. В. Дем’яненко — пт, 17 тра 2024 00:00:00 +0300

This study conducts a comparative analysis of computational modelling methods, focusing on the transcription factor stress tolerance WRKY2 in wheat (Triticum aestivum). WRKY2 plays a vital role in stress response mechanisms in wheat. Utilizing advanced techniques such as machine learning (in particular, AlphaFold) and homology modelling, the efficacy of these methods in elucidating the structural and functional aspects of WRKY2 was compared. Our findings provide insights into the most suitable computational strategies for studying stress tolerance mechanisms mediated by WRKY2, contributing to the enhancement of crop resilience against environmental stresses.

АНАЛІЗ ЕКОНОМІЧНОЇ ЕФЕКТИВНОСТІ ТА ДОЦІЛЬНОСТІ ВИКОРИСТАННЯ РІЗНИХ СИРОВИННИХ МАТЕРІАЛІВ ДЛЯ ВИРОБНИЦТВА БІОЕТАНОЛУ

О. В. Піскун — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the study of the feasibility of using different types of raw materials for the production of bioethanol based on the availability and cost of raw materials in Ukraine, environmental benefits, the economic feasibility of using raw materials and the complexity of the production process.

ВИКОРИСТАННЯ ПРЕПАРАТІВ TRICHODERMA ДЛЯ ЗНИЖЕННЯ АКТИВНОСТІ ФІТОПАТОГЕННИХ МІКРООРГАНІЗМІВ ПІД ЧАС КОМПОСТУВАННЯ РОСЛИННИХ РЕШТОК ОВОЧЕВИХ КУЛЬТУР

В. С. Рудь, Л. С. Зубченко — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the use of Trichoderma to accelerate the composting process and reduce the activity of phytopathogenic microorganisms. The article presents the results of determining the glucose content in the soil during composting at different time intervals, which is an indirect indicator of the activity of cellulolytic enzymes of Trichoderma.

КВАНТОВО-ХІМІЧНІ РОЗРАХУНКИ АНТИОКСИДАНТНИХ МЕХАНІЗМІВ 2.3-ДИГІДРОКСИ-6-МЕТИЛ-(4Н)-ПІРАН-4-ОНУ – МЕТАБОЛІТУ БАКТЕРІЙ РОДУ BACILLUS

І. О. Скороход, У. Ерденецогт, Ю. П. Горго — пт, 17 тра 2024 00:00:00 +0300

Quantum chemical analysis of the antioxidant and antiradical properties was performed for the optimized structures of the 2,3-dihydro-3,5-dihydroxy-6-methyl-(4H)-pyran-4-one (DDMP). The determined indicators of the course of antioxidant mechanisms showed that the dominant mechanisms for DDMP are SET (Single electron transfer) and SET-PT (Single-Electron Transfer followed by Proton Transfer, SET-PT), and DDMP is a member of the group of medium-strength antioxidants.

ДОСЛІДЖЕННЯ ТЕХНОЛОГІЧНИХ ТА БІОХІМІЧНИХ ХАРАКТЕРИСТИК АКТИВНОГО МУЛУ ОЧИСНИХ СПОРУД

Т. Л. Сулейко — пт, 17 тра 2024 00:00:00 +0300

Some characteristics of the activated sludge of the treatment plant were established, in particular, it is the concentration of activated sludge, sludge index and dehydrogenase activity. It has been established that the conditions of the semi-continuous process of wastewater treatment ensure greater efficiency of the treatment plant.

A COMPARATIVE ANALYSIS OF CONVENTIONAL AND MICROALGAE BIOFUELS PRODUCTION

O. Stroganov, M. Rybak, R. Hryhoriev, V. Zhukova — пт, 17 тра 2024 00:00:00 +0300

In recent years the intensive use of petroleum fuels has caused a major environmental impact, which prompted the search for alternative energy sources, like biofuels from crops and microalgae. This study compares the microalgae and crops in terms of annual productivity of biofuels and land usage. It was concluded biofuel production using microalgae requires on average 100-fold less area than crops.

IDENTIFICATION OF PHA-PRODUCING BACILLI AND OPTIMIZATION OF PHA BIOSYNTHESIS

A. Kharchuk, M. Kharkhota, M. Kharchuk, J. Brindza, L. Avdieieva — пт, 17 тра 2024 00:00:00 +0300

Polyhydroxyalkanoates present a promising alternative to traditional plastics. This study focused on characterizing two PHA-producing Bacillaceae family strains. The bacteria's ability to produce PHA granules was confirmed by microscopy and GCMS. Identification was based on chemotaxonomic analysis. B. weihenstephanensis 5w2 demonstrated the highest PHA yields when cultivated with glucose.

ПОБУДОВА ДЕРЕВА РІШЕНЬ ДЛЯ ПЕРЕДБАЧЕННЯ ФОРМИ БІОГЕННИХ МАГНІТНИХ НАНОЧАСТИНОК У МАГНІТОТАКСИСНИХ БАКТЕРІЯХ

К. Ю. Хахно, С. В. Горобець — пт, 17 тра 2024 00:00:00 +0300

Magnetic nanoparticles (MNPs) play key roles in targeted therapies and other fields. Magnetotactic bacteria (MTB) produce diverse biogenic magnetic nanoparticles (BMNs), but characterizing their shapes is challenging. The study develops advanced classification models combining decision tree analysis and protein characterization, improving our understanding of BMN shapes.

ДОСЛІДЖЕННЯ ВПЛИВУ КОМПЛЕКСІВ МЕТАЛІВ НА ВИХІД БІОГАЗУ

А. А. Шаповалова, Н. Б. Голуб — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the study of the influence of microelements (metal ions) on the production of biogas. Solutions of metal complexes – lysinates of chromium and nickel were used as additives. As a result of the study, it was shown that the addition of nickel lysinate (5 mg/l per metal) increases the yield of biogas by activating microbiological processes and stimulating methanogens.

БІОТЕХНОЛОГІЧНІ РЕСУРСИ ДЛЯ РОЗВИТКУ БІОПАЛИВ В СВІТІ І В УКРАЇНІ

Л. Ю. Авдєєва, А. А. Макаренко, Г. В. Декуша — пт, 17 тра 2024 00:00:00 +0300

The article is devoted to current trends in the production of biofuels for the dynamic development of the economy of various countries. The need for the production of alternative energy sources is substantiated. The state and directions of development of biofuel production from various types of raw materials have been studied. The prospects of using second-generation biofuels from lignin-cellulosic raw materials have been proven.

ВПЛИВ УЛЬТРАЗВУКУ НА ВИРОБНИЦТВО БІОЕТАНОЛУ

К. О. Байдур, А. В. Стецюк, Н. К. Коломацький — пт, 17 тра 2024 00:00:00 +0300

Theoretical grounds for the prospects of using biofuels are provided. The analysis focuses on the efficiency features of bioethanol production, with particular attention to the impact of ultrasonic irradiation during enzymatic hydrolysis of biomass. It is demonstrated that cavitation flow under high acoustic pressure facilitates the uniform distribution of the investigated medium.

СУЧАСНЕ ОБЛАДНАННЯ ДЛЯ ЕКСТРАГУВАННЯ

К. В. Гончаренко, Л. Ю. Авдєєва — пт, 17 тра 2024 00:00:00 +0300

This research assesses modern extraction technologies like supercritical CO², ultrasonic, and microwave-assisted methods, focusing on their efficiency, environmental benefits, and economic impact across industries such as pharmaceuticals and food production. The study underscores their role in enhancing sustainability and product quality in modern industrial applications.

INFLUENCE OF ULTRASONIC CAVITATION ON THE STRUCTURE OF PLANT RAW MATERIAL

Z. Ostapenko, K. Honcharenko — пт, 17 тра 2024 00:00:00 +0300

During ultrasonic extraction, the process of formation and implosion of cavitation bubbles takes place in the solvent. This process is accompanied by physical phenomena, such as high-speed solvent flows and shock waves, which lead to the enlargement of existing cracks and the formation of new cracks in the plant raw material. Physical processes occurring inside the plant raw material during ultrasonic cavitation contribute to the intensification of the process of extraction of biologically active substances.

ВИКОРИСТАННЯ НАНОТЕХНОЛОГІЙ У ДІАГНОСТИЦІ І ЛІКУВАННІ ОНКОЛОГІЧНИХ ЗАХВОРЮВАНЬ

К. В. Казьміна, О. В. Сохар, О. П. Кузь — пт, 17 тра 2024 00:00:00 +0300

This article is devoted to the study of the role of innovative technologies in solving the problem of oncology, the processes of using nanotechnology in the diagnosis of oncological diseases and the further development of a plan for their treatment thanks to the wide range of properties of nanoparticles.

ТЕХНОЛОГІЯ УЛЬТРАЗВУКОВОГО РУЙНУВАННЯ МІКРОВОДОРОСТЕЙ У ВИРОБНИЦТВІ БІОПАЛИВА

О. О. Колісніченко — пт, 17 тра 2024 00:00:00 +0300

My research focuses on ultrasonic microalgae destruction for biofuel, comparing it with other methods like mechanical and chemical biomass destruction. I analyze its principles, advantages, and disadvantages to enhance biofuel production understanding and contribute to effective technology development.

EXPERIMENTAL DETERMINATION OF DIFFUSION COEFFICIENTS IN SEMICONDUCTOR POLYMER MEMBRANES

D. Khyzhna, M. Shafarenko — пт, 17 тра 2024 00:00:00 +0300

An experimental determination of the diffusion coefficient in polymer membranes is carried out. The advantages of using semipermeable membranes are given. The main aspects and advantages of pervaporation are clarified. The rate of solvent absorption by the polymer was determined. The equilibrium conditions of the solvent on polymer supports are revealed.

FEATURES OF HYDRODYNAMICS WHEN USING AN OPEN TURBINE MIXER WITH A SPECIAL BLADE SURFACE

A. S. Ruzhanskyi, S. I. Kostyk — пт, 17 тра 2024 00:00:00 +0300

This article investigates a turbine mixer in a bioreactor with surface-modified dimples to enhance mixing efficiency. Computational simulations using ANSYS software examined the impact of these dimples on fluid dynamics. Results confirm improved efficiency, supported by experimental validation. A novel approach to optimize the turbine mixer design for increased mixing performance.

STUDY OF THE SPECIFIC PRODUCTIVITY OF MEMBRANES DURING THE CONDUCT OF THE PERVAPARATION PROCESS FOR THE SEPARATION OF LIQUID CARBOHYDRATE MIXTURES

Ya. O. Tretyakov, V. P. Kosova — пт, 17 тра 2024 00:00:00 +0300

The report presents the prospects for the use of membranes in pervaporation processes for the separation of liquid carbohydrates and their properties in increasing the efficiency and reducing the energy consumption of such processes. The work is aimed at studying the effectiveness of using membranes in pervaporation processes, which is a key stage in the separation of complex mixtures of liquid carbohydrates.

REMOTE SUPPLY MIXING ENERGY TO THE LIQUID

S. Fesenko, D. Khyzhna — пт, 17 тра 2024 00:00:00 +0300

The main problems facing the equipment, where the cultivation process is to ensure the processes of heat and mass transfer in devices, presence of turbulent and stagnant zones, high energy consumption, low heat transfer coefficients when working with viscous fluids. For the purpose of experimental studies selected weighing the advantages of remote control heat transfer in bioreactors production lines using ultrasonic beam compared to contact methods.

RESEARCH ON THE EFFECTIVE HYDRODYNAMICS OF AN ADSORBER WITH RUBBER MEMBRANES

D. A. Hutsaliuk, V. M. Melnick, А. V. Yaremchuk — пт, 17 тра 2024 00:00:00 +0300

Аn installation for ethanol purification using membrane elements for separation of gas and liquid systems in adsorption processes is being investigated. The stability of membrane elements and the duration of their operation due to favorable hydrodynamic conditions are indicated. A mixer in the form of two circular coaxial discs of different diameters with spiral slots is proposed.