Біотехнологія XXI століття

ОСОБЛИВОСТІ МЕТАДАНИХ ДЛЯ ЗРАЗКІВ МАТЕРИНСЬКОЇ ЧАСТИНИ ПЛАЦЕНТИ ЗА УМОВ ФІЗІОЛОГІЧНОГО І УСКЛАДНЕНОГО ПРЕЕКЛАМПСІЄЮ ПЕРЕБІГУ ВАГІТНОСТІ

П. Д. Архипов, М. Ю. Оболенська — пт, 16 тра 2025 00:00:00 +0300

This study presents an overview of the GSE60438 dataset, which contains 125 placental samples obtained via cesarean section. Samples were grouped into control and preeclampsia cases, with further subdivision into early and late forms based on gestational age. Metadata including diagnosis, gestational age, maternal age, gravidity, parity, and infant sex were supplemented from original publications and systematically organized for comparative analysis.

ПІДВИЩЕННЯ ВМІСТУ ВІТАМІНУ B12 У БІОМАСІ CHLORELLA VULGARIS, ВИРОЩЕНІЙ НА ОРГАНІЧНИХ СЕРЕДОВИЩАХ

В. В. Борцюх — пт, 16 тра 2025 00:00:00 +0300

This study investigates the influence of nutrient medium type (mineral and organic) and light spectral composition on the growth of Chlorella vulgaris and its vitamin B₁₂ content. It was found that the combination of an organic substrate (beef liver extract) and blue light significantly enhances biomass level and promotes the intensive accumulation of the active form of vitamin B₁₂.

XENIUM IN SITU АНАЛІЗ КОРОНАРНИХ ВІДДІЛІВ ГОЛОВНОГО МОЗКУ НА МИШАЧІЙ МОДЕЛІ ХВОРОБИ АЛЬЦГЕЙМЕРА

Є. Р. Броднікова, Л. П. Дзигун — пт, 16 тра 2025 00:00:00 +0300

The article describes the Xenium in situ analysis of coronal brain sections in a mouse model of Alzheimer's disease. The obtained results suggest that spatial transcriptomics is a highly effective method for identifying microregions of functional preservation and pathological sites in the brain in cases of neurodegenerative diseases.

PROSPECTS OF USING AGRO-INDUSTRIAL WASTE FOR BIOFUEL PRODUCTION

V. Halysh, M. Hromnadska, P. Zubyk, I. Trus, О. Yashchenko — пт, 16 тра 2025 00:00:00 +0300

This review examines the prospects for using agro-industrial waste for biofuel production. Agro-industrial production is a source of various wastes that can have different components and properties. Plant stalks, husks, straw etc. can be used for biofuel production provided that effective methods are used to decompose biomass into its main components and convert them into functional monomers.

РОЛЬ ВИБОРУ СИРОВИНИ ДЛЯ МІНІМІЗАЦІЇ H₂S У БІОГАЗІ

Є. К. Гоман, М. Ю. Козар — пт, 16 тра 2025 00:00:00 +0300

The presence of hydrogen sulfide (H₂S) in biogas poses significant challenges due to its corrosive nature and toxicity. This study reviews the sulfur content of various biomass feedstocks and their influence on H₂S formation during anaerobic digestion. Understanding these relationships is crucial for optimizing biogas production and ensuring environmental safety.

ВИЗНАЧЕННЯ ЗВ’ЯЗКІВ МІЖ НИЗЬКОЧАСТОТНИМИ ВАРІАЦІЯМИ АТМОСФЕРНОГО ТИСКУ ТА РЕАКЦІЄЮ МЕТАХРОМАЗІЇ ВОЛЮТИНОВИХ ГРАНУЛ МІКРООРГАНІЗМІВ

Ю. П. Горго, М. М. Опаленик, О. М. Громозова — пт, 16 тра 2025 00:00:00 +0300

The conditions for monitoring the color changes of the metachromatic reaction of volutinous granules of the yeast Saccharomyces cerevisiae and the values of the geomagnetic field intensity at frequencies below 1 Hz for further determination of the correlation coefficients between them, at which significant changes in the reaction are noted.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ НОСІЇВ ІММОБІЛІЗОВАНИХ МІКРООРГАНІЗМІВ В БІОТЕХНОЛОГІЯХ ОЧИЩЕННЯ СТІЧНИХ ВОД

А. О. Гриневич, Л. А. Саблій — пт, 16 тра 2025 00:00:00 +0300

This article presents a study on the efficiency of a "Brush-type carrier" for microorganism immobilization in a laboratory bioreactor simulating wastewater treatment conditions. Methods for determining carrier dry weight and biomass concentration are described. Based on three experimental series, the average biomass concentration was determined, and structural features and attachment stability of the biofilm were analyzed.

БІОІНФОРМАТИЧНИЙ ПОШУК ПРОБІОТИЧНИХ МІКРООРГАНІЗМІВ ДЛЯ БІОГЕНЕЗУ НАНОЧАСТИНОК

А. С. Гуменюк, С. В. Горобець — пт, 16 тра 2025 00:00:00 +0300

Research shows probiotics' potential for nanoparticle biosynthesis, though mechanisms remain unclear. Bioinformatic analysis of 199 probiotic proteomes identified homologs of key biomineralization proteins from Magnetospirillum gryphiswaldense MSR-1. Most probiotics used for green NP synthesis possess genetic mechanisms based on homologous proteins from MSR-1 magnetotaxis bacteria.

CHARACTERISTICS OF A BIOFILM ON A CARRIER FOR WASTEWATER TREATMENT

V. Zhukova — пт, 16 тра 2025 00:00:00 +0300

The research investigates the characteristics of a biofilm formed on a carrier for use in the processes of domestic wastewater treatment. The aim of the study was to determine the quantitative indicators of biomass immobilized on the carrier surface. It was found that the average concentration of biomass on the carrier surface reached 5425 mg/dm³. The results obtained indicate the prospects of using the studied carrier for effective wastewater treatment.

ВСТАНОВЛЕННЯ ОПТИМАЛЬНИХ ПАРАМЕТРІВ ОЧИЩЕННЯ СТІЧНИХ ВОД З ВИКОРИСТАННЯМ МЕМБРАННОГО БІОРЕАКТОРА

К. С. Кириченко, Л. А. Саблій — пт, 16 тра 2025 00:00:00 +0300

This study used GPS-X to optimize membrane bioreactor settings for municipal wastewater treatment. 36 scenarios tested filtration and washing durations. The best result—30 min filtration, 180 s washing — minimized transmembrane pressure (1.586 kPa) and reduced ammonium and nitrate levels to 0.13 and 11.36 mg/dm³, respectively.

ВПЛИВ КОМПЛЕКСІВ ІОНІВ ФЕРУМУ (Fe²⁺ ТА Fe³⁺) ТА ОСВІТЛЕННЯ НА МЕТАБОЛІЗМ CHLORELLA VULGARIS

С. О. Ковальова, Н. Б. Голуб — пт, 16 тра 2025 00:00:00 +0300

This study investigates the combined effects of light spectra and excess iron (Fe²⁺, Fe³⁺) on the accumulation of bioactive compounds in Chlorella vulgaris. Red-blue and white LED lighting with Fe²⁺, Fe³⁺ supplementation significantly influenced chlorophyll and carotenoid synthesis, highlighting the importance of optimizing cultivation conditions.

ВПЛИВ РІЗНИХ ДОВЖИН ХВИЛЬ СВІТЛА НА ВМІСТ ХЛОРОФІЛУ В CHLORELLA VULGARIS

Н. В. Козачок, Н. Б. Голуб — пт, 16 тра 2025 00:00:00 +0300

In the experiment, C. vulgaris was cultivated for 49 days in 1.5 dm³ reactors (15–17 °C) with weekly CO₂ aeration under LEDs in various red, green, and blue light ratios. The red-dominant regime produced the highest total chlorophyll and a/b ratio (up to 62.8 mg/g and 0.91), while excluding green light reduced pigment levels, underscoring its stabilizing role.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ АГРОВІДХОДІВ У ВИРОБНИЦТВІ ПАКУВАЛЬНИХ МАТЕРІАЛІВ

О. А. Корнелюк, М. Ю. Козар — пт, 16 тра 2025 00:00:00 +0300

In response to increasing environmental concerns and limited access to imported materials, this study explores the use of agricultural waste as natural reinforcing fillers for starch-based bioplastics. The results demonstrated that fillers like ground peanut shells and corn residues improve the mechanical strength and flexibility of bioplastics, with compressed sunflower husk granules offering the highest hardness. These findings support the development of cost-effective, biodegradable materials using locally available resources in Ukraine.

ТЕОРЕТИЧНИЙ АНАЛІЗ ЕФЕКТИВНОСТІ АНАЕРОБНОГО ЗБРОДЖУВАННЯ РІДКОЇ ГНОЇВКИ СВИНЕЙ ІЗ СОНЯШНИКОВИМ ЛУШПИННЯМ

О. О. Костюк, І. В. Дем’яненко — пт, 16 тра 2025 00:00:00 +0300

A theoretical analysis of the efficiency of co-anaerobic digestion of pig slurry and sunflower husks was conducted. It was shown that the addition of husks optimizes the C:N ratio and increases methane yield, confirming the feasibility of using such a mixture for biogas production.

ГРИБИ-АНТАГОНІСТИ, ЯК АЛЬТЕРНАТИВА ХІМІЧНИМ ФУНГІЦИДАМ У ЗАХИСТІ РОСЛИН

І. В. Кошеля, Т. В. Чекиш — пт, 16 тра 2025 00:00:00 +0300

This work examines the effectiveness of using antagonistic fungi as a biological plant protection agent. Research and literature were analyzed. Preparations based on Trichoderma viride, Chaetomium cohliodes, and Fomes fomentarius were used. The experimental part showed positive dynamics, improvement of soil microbiota, high effectiveness in combating phytopathogens, and ecological safety.

ВИВЧЕННЯ ДИФЕРЕНЦІЙНОЇ ЕКСПРЕСІЇ ГЕНІВ У ПЛАЦЕНТІ ЗА ФІЗІОЛОГІЧНОЇ ВАГІТНОСТІ

С. О. Левченко, М. Ю. Оболенська — пт, 16 тра 2025 00:00:00 +0300

This study focuses on the identification and functional interpretation of genes with significantly altered expression in the placenta between the first and second trimesters and third and second trimesters of pregnancy. Differential expression analysis was performed using microarray data and visualized via MA and Volcano plots. Top 20 DE-genes were selected based on log2FC and adjusted P-value and functionally annotated. The findings contribute to understanding molecular changes in normal placenta and may have future diagnostic value.

ПРОБЛЕМИ ОЧИЩЕННЯ СТІЧНИХ ВОД ПТАХОФАБРИК ТА ШЛЯХИ ЇХ ВИРІШЕННЯ

К. О. Лівінська — пт, 16 тра 2025 00:00:00 +0300

The current problem of pollution of water bodies by wastewater from poultry farms, which contain high concentrations of organic and inorganic substances, is considered. Insufficiently effective treatment of such wastewater leads to a decrease in the quality of natural waters and degradation of ecosystems. An analysis of modern methods of wastewater treatment, including mechanical, physicochemical and biological methods, is carried out.

USE OF ALGAE FOR BIOFUEL PRODUCTION: REVIEW OF INTERNATIONAL EXPERIMENTAL STUDIES

K. V. Maksymenko — пт, 16 тра 2025 00:00:00 +0300

The paper presents experimental studies on algae-based biofuels in Japan, the U.S., India, Europe, and beyond. Algae show high potential for renewable fuel production due to high yield, CO₂ capture, and non-arable land use. Case studies illustrate strain, cultivation, and conversion innovations.

СУЧАСНІ ТЕХНОЛОГІЇ БІОЛОГІЧНОГО ОЧИЩЕННЯ МІСЬКИХ СТІЧНИХ ВОД ВІД СПОЛУК АЗОТУ

С. В. Прокопенко — пт, 16 тра 2025 00:00:00 +0300

The article reviews modern biological wastewater treatment technologies, focusing on nitrogen compound removal. It covers the A/O, A2/O, and Bardenpho processes, analyzing their design, efficiency, and nitrogen removal capabilities. The comparative analysis helps identify their advantages, limitations, and suitable application conditions for optimal wastewater management and environmental protection.

GENETIC VARIABILITY OF VESICULAR STOMATITIS PHOSPHOLIPID GENE ISOLATED FROM VARIOUS HOSTS

A. P. Nyzhenets, L. B. Zelena, N. V. Tkachuk, S. D. Zahorodnia — пт, 16 тра 2025 00:00:00 +0300

The study analyzed the genetic heterogeneity of the vesicular stomatitis virus (VSV) phospholipid gene among isolates obtained from various host and different geographical regions. 40 nucleotide sequences were analyzed with MEGA 10. Results showed the high level of nucleotide polymorphism of VSV phospholipid gene and differentiation of strains due to their serotype.

СУЧАСНІ БІОТЕХНОЛОГІЇ ОЧИЩЕННЯ СТІЧНИХ ВОД З ВИКОРИСТАННЯМ БІОРЕАКТОРІВ З ІММОБІЛІЗОВАНИМИ МІКРООРГАНІЗМАМИ

К. Ю. Самченко — пт, 16 тра 2025 00:00:00 +0300

The abstract examines global water scarcity and increasing wastewater generation due to rising water demand and human activities. The Moving Bed Biofilm Reactor (MBBR) is studied as an effective biological treatment solution. The design and operation of MBBR systems with suspended plastic biofilm carriers are discussed, focusing on their role in enhancing biomass concentration and nutrient removal. The structure and function of MBBR aeration tanks are analyzed, along with the characteristics of various commercial plastic carriers.

ФІТОРЕМЕДІАЦІЯ ЯК ЕЛЕМЕНТ СТРАТЕГІЇ СТАЛОГО ЗЕМЛЕКОРИСТУВАННЯ

О. І. Сидоренко — пт, 16 тра 2025 00:00:00 +0300

This article highlights phytoremediation as a sustainable method for restoring soils contaminated during military operations in Ukraine. It examines the ecological, economic, and biotechnological aspects of using plants for detoxifying war-affected areas, emphasizing its role in post-conflict land rehabilitation.

ВИКОРИСТАННЯ СИСТЕМИ CRISPR-CAS9 ДЛЯ ПОКРАЩЕННЯ ВИРОБНИЦТВА БІОПАЛИВА

Я. А. Смірнова, Л. В. Маринченко — пт, 16 тра 2025 00:00:00 +0300

The rising global energy demand and the environmental impact of fossil fuels emphasize the need for renewable sources. Biofuels are a promising alternative, and recent advances in genome editing, particularly CRISPR-Cas9, enable precise improvements in the mechanisms of biomass and lipid production. This technology helps to enhance biofuel yields, reduce costs, and support the development of sustainable energy solutions.

ПОРІВНЯЛЬНА ХАРАКТЕРИСТИКА МЕТОДІВ АБСОЛЮТИЗАЦІЇ БІОЕТАНОЛУ: АЗЕОТРОПНОЇ РЕКТИФІКАЦІЇ ТА ЗНЕВОДНЕННЯ БІОЕТАНОЛУ НА МОЛЕКУЛЯРНИХ СИТАХ

В. В. Спаська, Д. С. Колтишева — пт, 16 тра 2025 00:00:00 +0300

These theses are devoted to a comparison of the most well-known methods of bioethanol absolutization: alcohol rectification and the method of alcohol reduction on molecular sieves. As a result of a review of their positive and negative characteristics, conclusions are drawn about the feasibility of using each technology.

НАЙПРОСТІШІ ЯК БІОІНДИКАТОРИ ЯКОСТІ АКТИВНОГО МУЛУ

К. М. Стогній — пт, 16 тра 2025 00:00:00 +0300

This research is devoted to analyzing of the state of active sludge from Bortnytsia aeration station. The analysis is conducted by identifying microorganisms, specifically the Protozoa and describing their role and contribution in the active sludge treatment of wastewater.

USING ORGANIC CARRIERS FOR BIOFILMS IN WASTEWATER TREATMENT

A. Tarnavska, V. Zhukova — пт, 16 тра 2025 00:00:00 +0300

This review explores sustainable natural and waste-derived materials as carriers for microbial biofilms in wastewater treatment. Materials like luffa fibers, clays, corncobs, cigarette filters, and textile waste support biofilm growth and pollutant removal. Their potential use in Ukraine aligns with the principles of circular bioeconomy and promotes sustainable wastewater treatment.

ЗМІНИ ЩІЛЬНОСТІ КУЛЬТУР ПРЕДСТАВНИКІВ РОДУ RHODOTORULA ЗА КОРОТКОЧАСНОЇ ДІЇ N-(ФОСФОНОМЕТИЛ)ГЛІЦИНУ

А. В. Тихенька, Л. М. Васіна — пт, 16 тра 2025 00:00:00 +0300

This article investigates the effect of short-term glyphosate exposure on the culture density of Rhodotorula species. Changes in microbial growth under different herbicide concentrations were assessed, enabling the evaluation of yeast tolerance to glyphosate and their potential for bioremediation.

ПЕРЕДБАЧЕННЯ ФОРМИ БІОГЕННИХ МАГНІТНИХ НАНОЧАСТИНОК У МАГНІТОТАКСИСНИХ БАКТЕРІЯХ ЗА ДОПОМОГОЮ ДЕРЕВ РІШЕНЬ ТА ВИПАДКОВИХ ЛІСІВ

К. Ю. Хахно, С. В. Горобець — пт, 16 тра 2025 00:00:00 +0300

Predicting biogenic magnetic nanoparticle shape is crucial for magnetogenetics. This study explores machine learning, specifically Decision Trees and Random Forests, for efficient shape classification of magnetosome crystals. Cross-validation demonstrates high accuracy, suggesting machine learning's potential for rapid shape prediction in bio-magnetic applications.

THE EFFECT OF BISPHENOL A ON THE LEVEL OF THIOBARBITURIC ACID REACTIVE SUBSTANCES AND CATALASE ACTIVITY IN CORYNEBACTERIUM GLUTAMICUM

M. A. Shchepanovska, L. M. Vasina — пт, 16 тра 2025 00:00:00 +0300

The effect of Bisphenol A (BPA) on oxidative stress markers in Corynebacterium glutamicum was evaluated. A concentration-dependent increase in thiobarbituric acid reactive substances levels indicated lipid peroxidation. Catalase activity was also elevated, reflecting antioxidant system activation. These results suggest C. glutamicum as a potential biomarker for environmental toxicity and bioremediation studies.

ВИКОРИСТАННЯ БІОТЕХНОЛОГІЧНИХ ПРИЙОМІВ ДЛЯ ВИЛУЧЕННЯ ГУМІНОВМІСНИХ РЕЧОВИН З БУРОГО ВУГІЛЛЯ

Л. Ю. Авдєєва, А. А. Макаренко, Т. Я. Турчина, Г. В. Декуша — пт, 16 тра 2025 00:00:00 +0300

This paper examines the advantages of applying biotechnological approaches for the extraction of humic substances. Microbial biotransformation of brown coal waste represents an environmentally sustainable technique, allowing for the recovery of humic acids with enhanced biological activity compared to conventional extraction methods.

NEW WAYS OF PRACTICAL APPLICATION OF ANTIMICROBIAL PHENAZINE PIGMENTS

M. Andreenkov, V. Klochko — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to analysis of relevant information about phenazine pigments, their properties, and novel directions of practical application. The main value of phenazines lies in their natural origin, ecological safety, and multifunctionality. Their practical use includes biocontrol in agriculture, integration into microbial fuel cells, and application in electrochemical sensors.

КУЛЬТИВУВАННЯ SCHIZOPHYLLUM COMMUNE НА СЕРЕДОВИЩАХ З РІЗНИМИ ДЖЕРЕЛАМИ АЗОТУ

Д. О. Белінська, В. М. Ліновицька — пт, 16 тра 2025 00:00:00 +0300

The article is devoted to the study of the influence of different nitrogen sources on the production of biomass and exopolysaccharides in two strains of the wood-destroying basidiomycete Schizophyllum commune. The studied strains of S. commune were cultivated on synthetic medium with different nitrogen sources. The highest amount of schizophyllan was observed on media with peptone and ammonium nitrate.

ВПЛИВ ПРОБІОТИКІВ НА ОСНОВІ LACTOBACILLUS ТА BIFIDOBACTERIUM НА СИМПТОМИ ДЕПРЕСІЇ ТА ТРИВОГИ

А. В. Білошапка, Д. С. Колтишева — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to evaluate the strain-specific effects of probiotics on relieving depressive and anxiety symptoms due to the microbiota-gut-brain axis (MGBA). Probiotic supplementation targeting the MGBA offer potential benefits for managing depression and anxiety. However, more clinical studies are necessary to fully understand and the therapeutic potential of the MGBA in neurological health and disease.

ВИЗНАЧЕННЯ ВМІСТУ ПОЛІФЕНОЛЬНИХ СПОЛУК У ЕКСТАКТАХ ECHINACEA PURPUREA

А. І. Блізніченко, Р. О. Петріна — пт, 16 тра 2025 00:00:00 +0300

Echinacea purpurea is a perennial plant rich in alkylamides, polysaccharides, flavonoids and phenolic compounds. It has immunomodulatory, antiviral, antibacterial and antioxidant effects. The aim of this study was to determine the content of polyphenolic compounds extracted from the aerial parts of Echinacea purpurea (stem and flowers). The total content of polyphenols in the extracts was determined spectrophotometrically using gallic acid as a standard.

ТЕРАПЕВТИЧНИЙ ПОТЕНЦІАЛ LAETIPORUS SULPHUREUS: ОГЛЯД БІОЛОГІЧНО АКТИВНИХ СПОЛУК

Є. Р. Броднікова, Л. П. Дзигун — пт, 16 тра 2025 00:00:00 +0300

The article provides a review of current studies of the therapeutic potential of Laetiporus sulphureus. The discovery of biologically active compounds with antitumor, antioxidant, anti-inflammatory and immunomodulatory properties opens up potential applications in medicine and the food industry.

ОПТИМІЗАЦІЯ СКЛАДУ СЕРЕДОВИЩА ДЛЯ ІНОКУЛЯЦІЇ В AGROBACTERIUM-ОПОСЕРЕДКОВАНІЙ ТРАНСФОРМАЦІЇ ТЮТЮНУ

В. А. Вернидуб, Г. В. Харчук, М. Ф. Парій, М. С. Дзуг, І. І. Гринюк — пт, 16 тра 2025 00:00:00 +0300

The study shows that the composition of the inoculation medium plays a key role in the efficiency of Agrobacterium-mediated transformation in Nicotiana tabacum. By comparing different media, it was determined that a formulation based on 1/2 MS supplemented with magnesium sulfate significantly improves transformation outcomes.

ЗАСТОСУВАННЯ ДЕКСТРАНАЗИ У СКЛАДІ ЗУБНИХ ПАСТ

В. С. Віннічук — пт, 16 тра 2025 00:00:00 +0300

This study explores dextranase, an enzyme that breaks α-(1–6)-D-glycosidic bonds in dextran, for caries prevention and plaque control. Sourced from fungi and marine bacteria, it shows promise in oral care, especially marine forms with higher stability in alkaline conditions. The study highlights dextranase's potential in managing biofilms and emphasizes the importance of further research to improve clinical efficiency and durability.

АНТИМІКРОБНИЙ ЕФЕКТ ЕФІРНОЇ ОЛІЇ, ЩО ПРОДУКУЄТЬСЯ ГРИБОМ EREMOTHECIUM ASHBYI

Р. Р. Войтенко — пт, 16 тра 2025 00:00:00 +0300

This article is a review of relevant scientific literature on the cytotoxicity of essential oils of Eremothecium ashbyi, the mechanism of its action on microorganisms, and the application of its properties to preserve food from spoilage by inhibiting harmful microorganisms.

ПОРІВНЯЛЬНИЙ АНАЛІЗ БАКТЕРІОФАГІВ РІЗНОГО СПЕКТРУ ДІЇ ЗА ЛІТИЧНОЮ АКТИВНІСТЮ ПРОТИ ШТАМІВ STAPHYLOCOCCUS AUREUS ЯК АЛЬТЕРНАТИВА АНТИБІОТИКОТЕРАПІЇ

О. С. Голікова, Ю. С. Крищук — пт, 16 тра 2025 00:00:00 +0300

The global spread of multidrug-resistant Staphylococcus aureus, including methicillin-resistant strains (MRSA) and isolates with inducible macrolide-lincosamide-streptogramin B (iMLSB) resistance, poses a significant clinical and veterinary challenge. This study assessed the in vitro lytic efficacy of bacteriophages with varying host specificities against phenotypically diverse St. aureus strains.

ANALYSIS OF BIOMASS ACCUMULATION BY LACTIC ACID BACTERIA ISOLATED FROM PLANT RAW MATERIALS

D. Holubchyk, О. Dugan, О. Potemska, А. Khablenko — пт, 16 тра 2025 00:00:00 +0300

These theses are devoted to the analysis of dry weight biomass in potential probiotic lactic acid bacterial strains isolated from maize, barley, and oat sourdoughs. The results show that the sample weights are 2,04 ± 0,16, 2,16 ± 0,05, and 2,12 ± 0,11 g/L, with no statistically significant differences among them. Further research should focus on determining the number of viable cells (CFU/mL) in these cultures and optimizing the nutrient media to suit their needs.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ КАЛЬЦІЙ ФОСФАТІВ АПАТИТОВОГО ТИПУ ДЛЯ СОРБЦІЇ НУКЛЕЇНОВИХ КИСЛОТ

Я. А. Горюнов, Д. К. Прохоренко — пт, 16 тра 2025 00:00:00 +0300

Hydroxyapatite is a promising material for nucleic acid extraction due to its phosphate affinity and biocompatibility. Modifications to HAp improve binding selectivity and protect biomolecules from degradation. Such optimized sorbents open new possibilities for applications in rapid molecular diagnostics without complex sample preparation and environmental DNA monitoring.

RESEARCH ON THE INFLUENCE OF THE ANTISCALANT ON THE BIOFOULING PROCESS

М. Hromnadska, І. Trus — пт, 16 тра 2025 00:00:00 +0300

This work is devoted to the study of water stabilization treatment using the reagent RT-2024-3 in Kyiv tap water. This work also presents the results of an experimental study of the nature of biofouling on the surface of the glass materials using the antiscalant RT-2024-3.

ВПЛИВ БІЛКОВО-ВУГЛЕВОДНИХ ЗАХИСНИХ СЕРЕДОВИЩ НА ЗБЕРЕЖЕННЯ ЖИТТЄЗДАТНІСТІ МОЛОЧНОКИСЛИХ БАКТЕРІЙ В УМОВАХ СТРЕСОВИХ ТЕМПЕРАТУР

Є. І. Губар, І. М. Корнієнко — пт, 16 тра 2025 00:00:00 +0300

In this study, thermal stress during the drying of lactic acid bacteria LAB cells was investigated using the RV 05 basic 1-B rotary evaporator. The aim of the study was to select protein-carbohydrate protective media for the drying of probiotic cultures (genera Bifidobacterium and Lactobacillus) by evaporation. It was found that the most effective protective media were those supplemented with skim milk and either inulin or lactulose.

АНАЛІЗ ЕФЕКТИВНОСТІ АДСОРБЦІЙНОЇ ІММОБІЛІЗАЦІЇ ФЕРМЕНТНОГО ПРЕПАРАТУ «ПАНКРЕАТИН» ІЗ ЗАСТОСУВАННЯМ БАЗАЛЬТОВОГО ТУФУ ЯК ПРИРОДНОГО МІНЕРАЛЬНОГО НОСІЯ

Л. В. Гуменюк, Л. В. Худа — пт, 16 тра 2025 00:00:00 +0300

The efficiency of adsorption immobilization of the enzyme preparation Pancreatin on basalt tuff under different conditions was evaluated. It was found that mixing the enzyme solution with the carrier (40 rpm) leads to a 1,5-fold decrease in the immobilization efficiency compared to the static method. The maximum amount of pancreatin was immobilized during the 1-hour static procedure.

ТЕХНОЛОГІЧНІ АСПЕКТИ ЗБРОДЖУВАННЯ ОВЕЧОГО МОЛОКА ЗАКВАШУВАЛЬНИМИ КУЛЬТУРАМИ ІПРОВІТ

В. О. Дмитриченко, С. Г. Даниленко, Л. В. Маринченко — пт, 16 тра 2025 00:00:00 +0300

This study investigates the possibility of using the Iprovit starter cultures of the Institute of Food Resources of the NAAS of Ukraine for the fermentation of sheep milk. It was found that classical yogurt starters, which are used in the production of fermented milk’s products from cow's milk, are generally suitable for the fermentation of sheep milk, since they formed a clot (although longer), and the organoleptic indicators were almost the same.

ВМІСТ БІОЛОГІЧНО АКТИВНИХ КОМПОНЕНТІВ TRIFOLIUM PRATENSE L. В ЗАЛЕЖНОСТІ ВІД УМОВ ЗРОСТАННЯ

К. А. Довгопола, К. Г. Гаркава — пт, 16 тра 2025 00:00:00 +0300

The issues regarding the prospects of using Trifolium pratense L. as a medicinal plant raw material are highlighted. The content of ascorbic acid and flavonoids in the plant was determined. The effectiveness of the influence of meadow clover on the level of osmotic resistance of erythrocyte membranes was studied.

АНАЛІЗ ПОЖИВНИХ СЕРЕДОВИЩ ДЛЯ КУЛЬТИВУВАННЯ ПРОДУЦЕНТІВ РОДУ AZOSPIRILLUM

М. А. Дузінський — пт, 16 тра 2025 00:00:00 +0300

Azospirillum spp. are biofertilizers that enhance plant growth via nitrogen fixation and phytohormone production. This study evaluates optimized culture media for boosting biomass yield and suitability for inoculant formulation.

БІОХІМІЧНІ ПОКАЗНИКИ ТОПІНАМБУРУ ЗА ВПЛИВУ ІОНІВ КАДМІЮ

І. М. Задарко, О. А. Булій, Д. С. Шебунчак, В. В. Гусак — пт, 16 тра 2025 00:00:00 +0300

The study investigated the impact of various cadmium concentrations (0–5 mg/L) on biochemical parameters of Jerusalem artichoke in vitro. Increased pigments, polyphenols, and flavonoids indicated adaptive antioxidant responses to cadmium-induced oxidative stress.

EVALUATION OF PECTIN METHYL ESTERASE ACTIVITY OF THREE STRAINS OF T. VERSICOLOR IN SYNTHETIC MEDIUM

P. Zubyk, I. Klechak — пт, 16 тра 2025 00:00:00 +0300

The study examined the growth and pectin methylesterase activity of Trametes versicolor 353, 1689 and 5094 strains when cultivated on Norcrans synthetic medium. It was found that strain T. versicolor 353 is characterized by the highest level of biomass accumulation. At the same time, the maximum pectin methylesterase activity was recorded in strain T. versicolor 5094, which indicates its potential for further research.

АНАЛІЗ ТРАДИЦІЙНИХ ТА НОВИХ ШТАМІВ ЛАКТОБАКТЕРІЙ: ФУНКЦІОНАЛЬНІ ВЛАСТИВОСТІ ТА КЛІНІЧНИЙ ПОТЕНЦІАЛ

К. В. Казьміна, О. І. Яловенко — пт, 16 тра 2025 00:00:00 +0300

The study analyzes traditional and novel strains of Lactobacillus, Lacticaseibacillus, Lactiplantibacillus, Limosilactobacillus from the perspective of their functional properties and clinical potential. The work focuses on the biochemical and probiotic profiles of various strains and their potential application in therapy and microbiota modulation.

РОСТОВІ ВЛАСТИВОСТІ БАЗИДІОМІЦЕТІВ LAETIPORUS SULPHUREUS У ПОВЕРХНЕВІЙ КУЛЬТУРІ

Ю. А. Калюжний — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to the influence of the component composition of the nutrient media on the growth and enzymatic activity of Laetiporus sulphureus in surface culture was studied. It was found that extracts of birch sawdust and grape pomace slightly stimulate mycelial growth and significantly increase the activity of oxidoreductases, such as peroxidases.

PRODUCTION OF ANTISTAPHYLOCOCCAL ANTIBIOTIC BATUMIN UNDER CONDITIONS OF BATCH CULTIVATION PROCESS

U. Karpenko, V. Klochko — пт, 16 тра 2025 00:00:00 +0300

This work is aimed at studying the cultivation process of Pseudomonas batumici, and particularly on the yield of batumin – secondary metabolite with antibacterial activity. It also focuses on determining the activity of obtained batumin extract, separation of compounds by thin layer chromatography with subsequent bioautography for the identification of said compounds and their activity.

ПЕКТИНАЗОПРОДУКУЮЧІ АКТИНОМІЦЕТИ: СУЧАСНИЙ СТАН ДОСЛІДЖЕНЬ

Я. П. Коваленко — пт, 16 тра 2025 00:00:00 +0300

Pectinases are one of the most popular enzymes in the world. They are used in various industries. Given the growing demand for these enzymes, we are looking at the most efficient producers. For this review, I have chosen actinomycetes as the main group of producers. In addition, this review also looks at culture media and cultivation conditions.

СУЧАСНІ ПІДХОДИ ВИКОРИСТАННЯ БАКТЕРІОФАГІВ У БІОТЕХНОЛОГІЧНИХ ЦІЛЯХ

А. Ю. Корзун, К. І. Гордієнко, Т. М. Луценко — пт, 16 тра 2025 00:00:00 +0300

Despite of the development of various antibacterial drugs, the emergence of multidrug-resistant bacteria is a major problem today. Bacteriophages are agents of antibacterial treatment and have such advantages: specificity of lysis of target bacteria and low toxicity. Currently, there are a wide range of applications of these biological agents in the field of medicine, pharmacy and biotechnology.

ЦИТОТОКСИЧНІСТЬ ЛЕГОВАНИХ МЕТАЛАМИ КАЛЬЦІЙ ФОСФАТІВ АПАТИТОВОГО ТИПУ

А. М. Котляр, К. С. Онищенко, Н. Ю. Струтинська — пт, 16 тра 2025 00:00:00 +0300

The cytotoxic properties of nanohydroxyapatites are analyzed. Analysis of literature sources showed that the biocompatibility and cytotoxicity of metal-doped nanohydroxyapatites depend on the type of dopant ion, nanoparticle morphology, and material concentration. The relevance of these materials in biomedical applications is highlighted.

ФОРМУВАННЯ БІОПЛІВОК ПАТОГЕННИХ МІКРООРГАНІЗМІВ ЯК КЛЮЧОВИЙ ФАКТОР ХРОНІЧНИХ ІНФЕКЦІЙ: НОВІ МЕТОДИ ІНГІБУВАННЯ

І. В. Кошеля, А. І. Красилова — пт, 16 тра 2025 00:00:00 +0300

This article examines one of the causes of chronic infections – biofilm. Due to biofilm, chronic infections are difficult to treat and tend to recur. Therefore, the article presents an analysis of research on methods for inhibiting biofilm formation, particularly through the use of ultrasound and nanoparticles, which show promising results in disrupting biofilm structure.

ШЛЯХИ ЗНИЖЕННЯ НЕФРОТОКСИЧНОСТІ АНТИБІОТИКУ АМФОТЕРИЦИНУ В

У. В. Кректун — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to the analysis of the use of the antibiotic amphotericin B and methods to reduce its toxic effects. Amphotericin B is a very important antifungal agent in clinical practice, but its conventional deoxycholate form often causes severe side effects, the main of which is nephrotoxicity. The main method of reducing this effect is currently the use of lipid forms of the drug.

ВПЛИВ РІЗНИХ ДЖЕРЕЛ ВУГЛЕЦЮ НА РІСТ SCHIZOPHYLLUM COMMUNE ТА СИНТЕЗ ШИЗОФІЛАНУ

К. О. Криволап, В. М. Ліновицька — пт, 16 тра 2025 00:00:00 +0300

The article presents the results of experimental studies of Schizophyllum commune in submerged cultivation on the nutrient media for as a producer of exopolysaccharide schizophyllan. The influence of various carbon sources on biomass accumulation and polysaccharide biosynthesis has been investigated. As a result, maltose was identified as a promising carbon source in the medium for schizophyllan production for the S. commune strain.

ВПЛИВ РІЗНИХ ДЖЕРЕЛ КАРБОНУ ТА НІТРОГЕНУ НА КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНІ ПОКАЗНИКИ PLEUROTUS CITRINOPILEATUS ПРИ КУЛЬТИВУВАННІ НА АГАРИЗОВАНИХ ЖИВИЛЬНИХ СЕРЕДОВИЩАХ

О. В. Кузнецова, К. М. Власенко, С. В. Карпикова — пт, 16 тра 2025 00:00:00 +0300

The scientific paper presents studies on the influence of different carbon and nitrogen sources on the development of Pleurotus citrinopileatus (strain IВK-2160) mycelium when cultivated on an agar medium. It was found that the best carbon source for this strain is sucrose, and the best nitrogen source is ammonium sulfate. The average radial growth rate of mycelium was 1,7 mm/day.

PCR ANALYSIS OF STOMATA BIOGENESIS GENES IN COMMON WHEAT VARIETIES

пт, 16 тра 2025 00:00:00 +0300

The aim of this study was to develop primer systems for precise genotyping of bread wheat cultivars by stomatal biogenesis genes. Seven sets of primers covering different gene regions of EPF1-A1, EPF1-B1, EPF1-D1, EPF2-A1, and MUTE-D1, were designed to annotate genetic diversity among 72 wheat cultivars. The developedDNA markers represent effective, informative means for selecting drought tolerance germplasm donors to promote wheat breeding programs.

СУЧАСНІ НАПРЯМИ ОПТИМІЗАЦІЇ ПОЖИВНИХ СЕРЕДОВИЩ ДЛЯ ОТРИМАННЯ ІНОКУЛЯНТІВ НА ОСНОВІ BRADYRHIZOBIUM SP.

К. Р. Литовчак — пт, 16 тра 2025 00:00:00 +0300

Bradyrhizobium-based inoculants enhance soybean (Glycine hispida Maxim.) growth by boosting nitrogen fixation. Optimization of cultivation media via carbon sources, amino acids, and isoflavones mproves bacterial activity and symbiosis. These findings aid in developing efficient inoculants for sustainable agriculture.

ДОСЛІДЖЕННЯ АНТАГОНІСТИЧНОЇ АКТИВНОСТІ ШТАМУ LACTOCOCCUS LACTIS SUBSP. LACTIS

М. М. Михайленко , О. І. Потемська — пт, 16 тра 2025 00:00:00 +0300

The study of the antagonistic activity of the Lactococcus lactis subsp. lactis strain against pathogenic and opportunistic microbiota is a necessary condition for selecting cultures for bacterial preparations used in the production of fermented products. It was found that the Lactococcus lactis subsp. lactis strain did not affect the growth of Pseudomonas aeruginosa, Enterobacter aerogenes, and Proteus vulgaris. The most sensitive strain was Escherichia coli 055.

АНАЛІЗ РОСТУ МІКРОБНИХ ПОПУЛЯЦІЙ ПРИ ВИКОРИСТАННІ РІЗНИХ ДЖЕРЕЛ ВУГЛЕЦЮ

А. Ю. Науменко — пт, 16 тра 2025 00:00:00 +0300

The study investigates the effect of various carbon sources (glucose, fructose, galactose) on the growth of Lactobacillus rhamnosus under submerged cultivation. Statistical analysis confirmed glucose as the most effective substrate for biomass production and growth rate.

ВИКОРИСТАННЯ АНТОЦІАНІВ У СТВОРЕННІ pH ЧУТЛИВОЇ УПАКОВКИ ДЛЯ МОНІТОРИНГУ СВІЖОСТІ М’ЯСА

Є. С. Олійник — пт, 16 тра 2025 00:00:00 +0300

The article is devoted to the study of the potential use of anthocyanins as pH‑sensitive indicators in intelligent biodegradable packaging for monitoring meat freshness. The influence of anthocyanin concentration on the colorimetric and mechanical properties of the packaging film is analyzed.

ВПЛИВ МОДИФІКОВАНИХ КАЛЬЦІЙ ФОСФАТІВ АПАТИТОВОГО ТИПУ ТА ЇХ КОМПОЗИТІВ З ФЕРИТАМИ НА ГЕМОЛІЗ ЕРИТРОЦИТІВ

К. С. Онищенко , А. М. Котляр, І. І. Гринюк, Є. О. Комащенко , Н. Ю. Струтинська — пт, 16 тра 2025 00:00:00 +0300

The effect of apatite-type phosphates modified with trace elements (Na⁺, Mg²⁺, Zn²⁺), iron and calcium carbonate anion and their composites with ferrites of divalent metals on the degree of hemolysis of erythrocytes in vitro was studied. It was found that the resistance of sheep erythrocytes to acid hemolytic after preincubation with the studied samples differed and depended on the composition and amount of the sample.

3D-БІОДРУК ЕНДОМЕТРІАЛЬНИХ КОНСТРУКЦІЙ ДЛЯ ФУНКЦІОНАЛЬНОГО ВІДНОВЛЕННЯ МАТКИ

М. С. Отрода — пт, 16 тра 2025 00:00:00 +0300

This paper presents a literature analysis of biofabrication strategies for bilayer endometrial scaffolds, comparing hydrogel-based drug delivery systems and stem cell-loaded scaffolds. We examine biomaterial formulations, cellular compositions, release kinetics, and in vitro/in vivo functional outcomes to determine optimal approaches to uterine tissue regeneration.

ВПЛИВ МІКРОБІОТИ НАТИВНИХ ЗАКВАСОК НА ПОКАЗНИКИ ХЛІБА

Л. Я. Паляниця , А. Л. Бережнюк , Л. В. Ткаченко — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to the study of the influence of the microbiota of native spontaneous fermentation sourdoughs, obtained on the basis of rye and wheat flour with the addition of whole-wheatflour, on the indicators of finished bread. The results of the research can be used to develop optimal recipes of sourdough in bread technology with improved nutritional and organoleptic properties.

ПЕРСПЕКТИВИ ЗАСТОСУВАННЯ ПРОБІОТИКІВ ПРИ ГОСТРИХ ГАСТРОЕНТЕРИТАХ

В. В. Піць, С. О. Соловйов — пт, 16 тра 2025 00:00:00 +0300

In these theses, we investigated the effect of additional probiotic therapy on the number of days in the hospital for children with acute gastroenteritis. The study used retrospective data from children with rotavirus infection. The result is a comparison of the number of days in the hospital with different therapies for different severity of the disease.

HYDROLYSATES FROM HEMP SEEDS AS ALTERNATIVE SOURCE OF PROTEIN

A. Podolchak , Y. Tepla , R. Petrina , V. Havryliak — пт, 16 тра 2025 00:00:00 +0300

This article reveals the topic of plant proteins derived from legumes, oilseeds and pseudozerenes, offering a sustainable alternative and can be processed into functional foods. Away from the production of animal proteins that generate environmental problems. This study aims to optimize the enzymatic hydrolysis conditions for the production of protein hydrolysates from hemp seeds.

РОЗРОБКА СИСТЕМИ ПРАЙМЕРІВ ДЛЯ ВИЯВЛЕННЯ БАКТЕРІЙ РОДУ STAPHYLOCOCCUS МЕТОДОМ ПЛР У РЕАЛЬНОМУ ЧАСІ

Д. К. Прохоренко — пт, 16 тра 2025 00:00:00 +0300

In this work, a primer system were selected and optimized for the specific detection of a conserved DNA fragment of Staphylococcus spp. using real-time PCR. The results obtained demonstrate the high efficiency and strong potential of the developed system for application in molecular diagnostics.

ВИРОБНИЦТВО ПЕКТИНУ З ВТОРИННОЇ РОСЛИННОЇ СИРОВИНИ

В. О. Пустошкіна — пт, 16 тра 2025 00:00:00 +0300

This paper investigates the technological process of pectin production from secondary plant raw materials. Particular attention is paid to innovative methods of pectin extraction, such as ultrasonic, microwave, subcritical water extraction and the use of deep eutectic solvents. The presented results may be useful for improving existing and developing new technologies for processing plant biomass.

БІОГЕННІ МАГНІТНІ НАНОЧАСТИНКИ У ПУХЛИННИХ КЛІТИНАХ РІЗНОГО ПОХОДЖЕННЯ ДЛЯ ТЕРАПЕВТИЧНОГО ВИКОРИСТАННЯ

Є. А. Салацький, О. Ю. Горобець — пт, 16 тра 2025 00:00:00 +0300

This study examines biogenic magnetic nanoparticles (BMNs) in various tumor types through magnetization measurements. Results show significant variation: meningiomas and glioblastomas often contain higher BMN levels than normal tissues, while carcinomas show reduced levels. These differences provide a basis for potential targeting strategies in cancer therapy.

ВИВЧЕННЯ ЦИТОТОКСИЧНОЇ ДІЇ МЕТИЛ-6-АМІНОГЕКСАНОАТ ГІДРОХЛОРИДУ

М. П. Сметюх, С. О. Соловйов, А. С. Момот, О. П. Трохименко — пт, 16 тра 2025 00:00:00 +0300

This study presents an evaluation of the cytotoxic properties of methyl 6-aminocaproate hydrochloride in vitro using a hamster kidney cell model. Automated image analysis was applied for quantitative assessment of morphological changes and cell viability. The results confirm the efficiency of the implemented algorithm in detecting toxicity markers and demonstrate a stable toxicological profile of the tested compound.

ЗАСТОСУВАННЯ ФЕРМЕНТАТИВНОЇ АКТИВНОСТІ ГРИБІВ SCHIZOPHYLLUM COMMUNE ТА PLEUROTUS OSTREATUS У ЗНЕБАРВЛЕННІ СИНТЕТИЧНИХ СПОЛУК

А. М. Стецюк, О. Р. Козидуб, К. О. Байдур — пт, 16 тра 2025 00:00:00 +0300

This article is devoted to exploration of the potential of white rot fungi Schizophyllum commune and Pleurotus ostreatus in decolorizing synthetic dyes. Due to their ability to produce extracellular oxidative enzymes, these fungi offer an eco-friendly, cost-effective alternative for the bioremediation of industrial wastewater.

ОСОБЛИВОСТІ ЗАХИСНИХ СЕРЕДОВИЩ ДЛЯ ЛІОФІЛЬНОЇ СУШКИ МОЛОЧНОКИСЛИХ БАКТЕРІЙ LACTIPLANTIBACILLUS PLANTARUM

М. Д. Турчаненко — пт, 16 тра 2025 00:00:00 +0300

This study evaluates the effectiveness of different protective media in the lyophilization of lactic acid bacteria. Three formulations – based on soy hydrolysate, sodium caseinate, and maltodextrin – were tested. The soy hydrolysate-based medium with yeast extract and sucrose showed the highest cell survival rate (78 %). This research contributes to the optimization of probiotic preservation methods.

ОСОБЛИВОСТІ ВИБОРУ РОСЛИННИХ ПРОДУЦЕНТІВ ДЛЯ СИНТЕЗУ РЕКОМБІНАНТНИХ ТЕРАПЕВТИЧНИХ БІЛКІВ

Ю. Р. Удовенко, О. О. Овчаренко — пт, 16 тра 2025 00:00:00 +0300

Molecular farming is a promising technology for producing recombinant therapeutic proteins in plants in large volumes without the risk of accumulation of animal pathogens. This paper discusses the advantages and disadvantages of different plant species used for molecular farming, identifying optimal species for the production of recombinant proteins by transient expression, stable nuclear, and chloroplast transformation.

ОЦІНКА ВПЛИВУ МАЛЬТОЗИ НА РІСТ ШТАМУ LIMOSILACTOBACILLUS FERMENTUM

А. Д. Хабленко, О. М. Дуган, С. Г. Даниленко — пт, 16 тра 2025 00:00:00 +0300

The theses are dedicated to the study of the growth of the Limosilactobacillus fermentum strain in a medium with added maltose, acid production, and carbohydrate consumption assessment. The strain's ability for active growth and efficient carbohydrate utilization has been established, indicating a positive effect of maltose addition.

КОМБІНОВАНІ ЗАСОБИ НА ОСНОВІ МІКРОБНИХ СУБСТАНЦІЙ ДЛЯ ЛІКУВАННЯ ІНФІКОВАНИХ РАН

М. О. Шевченко — пт, 16 тра 2025 00:00:00 +0300

The article is devoted to the analysis of approaches to the development of modern means for the treatment of infected wounds, as well as the prospects for the use of biologically active compounds of microbial origin with different mechanisms of action. The prospects of the use of antimicrobial substances of microbial origin (enzyme complex and antibiotics) in the composition of soft dosage forms for the local treatment of infected wounds are shown.

ВИКОРИСТАННЯ СТОВБУРОВИХ КЛІТИН У ВІДНОВЛЕННІ ПОШКОДЖЕНИХ ТКАНИН ТА ОРГАНІВ

С. Ю. Шкуратова — пт, 16 тра 2025 00:00:00 +0300

Stem cell therapy is a leading field in regenerative medicine, offering great potential for restoring damaged tissues. Mesenchymal stem cells (MSCs), derived from bone marrow, fat tissue, or the umbilical cord, are especially valued for their ability to regenerate and repair various tissues in the body.

ІНДУКУВАННЯ НАДСИНТЕЗУ РИБОФЛАВІНУ EREMOTHECIUM ASHBYI РЕГУЛЯЦІЄЮ МЕТАБОЛІЧНИХ ШЛЯХІВ

З. С. Шопова, В. Ю. Поліщук — пт, 16 тра 2025 00:00:00 +0300

Eremothecium ashbyi is a natural producer of riboflavin, an essential water-soluble vitamin involved in redox reactions as a precursor of FMN and FAD. The study aimed to enhance riboflavin biosynthesis via UV irradiation and itaconate treatment. The highest yield of 531,61 mg/L was obtained on oil-based media, indicating the effectiveness of stress-induced stimulation and metabolic regulation.

ОЦІНКА ТОКСИЧНИХ ВЛАСТИВОСТЕЙ EREMOTHECIUM ASHBYI НА МОДЕЛЬНОМУ ОГАНІЗМІ DROSOPHILA MELANOGASTER

З. С. Шопова, В. О. Орлова, О. В. Проценко, В. Ю. Поліщук — пт, 16 тра 2025 00:00:00 +0300

In this article, the ability of the culture filtrate of Eremothecium ashbyi and the mycelium of E. ashbyi to exert a toxic effect on adults and individuals that have undergone a full development cycle (from egg to adult) of the model organism Drosophila melanogaster was evaluated. The absence of a toxic effect was shown.

CONDITIONED MEDIUMS IN REGENERATIVE MEDICINE

D. Shcherbak, T. Lutsenko — пт, 16 тра 2025 00:00:00 +0300

Conditioned medium (CM), a bioactive cell culture supernatant, has emerged as a promising cell-free therapeutic strategy in regenerative medicine. This review examines CM’s complex composition, production methods, mechanisms of action, and applications in tissue repair and immunomodulation, particularly from MSCs. It also addresses delivery strategies, regulatory issues, and challenges in clinical translation.

SYSTEM-BASED ANALYSIS OF PSEUDOMONAS SPP. FOR BIOTECHNOLOGICAL AND ECOLOGICAL APPLICATIONS

V. Klochko, M. Liulchak, O. Kobzysta, V. Babka — пт, 16 тра 2025 00:00:00 +0300

Pseudomonas species are ubiquitous bacteria that inhabit diverse ecological niches in both terrestrial and aquatic environments, and often live in association with plants and animals. They are characterized by a remarkable biosynthetic capacity and the production of a wide range of secondary metabolites, and they possess an extensive array of enzymes and metabolic pathways. Systems biology techniques and expanding genomic databases will greatly contribute to expanding the range of products generated through the manipulation of metabolic pathways.

SYSTEM-BASED ANALYSIS OF PSEUDOMONAS SPP. FOR BIOTECHNOLOGICAL AND ECOLOGICAL APPLICATIONS

V. Klochko, M. Liulchak, O. Kobzysta, V. Babka

СУЧАСНІ БІОТЕХНОЛОГІЇ НА ДОПОМОГУ ВИРОБНИЦТВУ БІОГАЗУ

М. В. Козловець

This article contains information about the most modern and interesting practical research that scientists in the field of bioenergy are currently solving in terms of biogas and biomethane production: TeCH4BiogasUp, Power-to-X, auto-generative high pressure digestion, improving biogas membrane purification technology, using nanoparticles to improve anaerobic digestion, modeling complex anaerobic digestion models and gradient optimization.

СУЧАСНІ БІОТЕХНОЛОГІЇ НА ДОПОМОГУ ВИРОБНИЦТВУ БІОГАЗУ

М. В. Козловець — пт, 16 тра 2025 00:00:00 +0300

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ БІОПАЛИВНИХ ЕЛЕМЕНТІВ В ЕКОБІОТЕХНОЛОГІЇ

Д. С. Колтишева — пт, 16 тра 2025 00:00:00 +0300

The deterioration of the environment, which is becoming more acute every year, poses a number of urgent tasks for scientists. Biofuel cells as bioelectrochemical systems in which current is generated by bioconversion of waste are promising in ecobiotechnology. Microbial fuel cells, microbial electrolysis cells, plant biofuel cells and a number of others are promising for solving the problems of waste disposal and electricity generation.

CLASSIFICATION AND FUNCTIONAL ANALYSIS OF GENE EXPRESSION DYNAMICS IN THE HUMAN PLACENTA DURING UNCOMPLICATED PREGNANCY

Y. О. Kukuruza, М. Yu. Obolenska — пт, 16 тра 2025 00:00:00 +0300

This article analyzes placental gene expression dynamics during pregnancy. 9 expression dynamics clusters were grouped into 4 “mirrored” groups. Functional analysis revealed that genes in these groups partake in immune, metabolic, structural, and developmental processes, offering insight into coordinated function of genes in placenta.

ULTRASONIC PRE-TREATMENT FOR ENHANCED AIR FILTRATION IN PHARMACEUTICAL CLEANROOMS

V. Shybetskyi, M. Kalinina, S. Semeniuk, D. Khyzhna — пт, 16 тра 2025 00:00:00 +0300

High-efficiency filters such as HEPA and ULPA are widely used but face challenges related to cost, pressure drop, and service life. The RESPIRATION project, implemented under the Horizon 2020 programme, addresses this issue by introducing ultrasonic pre-treatment before the filtration stage. The method relies on acoustic resonators embedded in the airflow path to induce agglomeration of PM2.5 and smaller particles, making them easier to capture and reducing the load on final filters. A novel simulation approach was developed to model ultrasonic emitter behaviour and sound field propagation using ANSYS software. The multi-module framework included Modal and Harmonic Acoustic analyses, with CFD implementation through boundary condition transfer. The proposed method enables more accurate energy distribution modelling and supports the design of scalable, energy-efficient pre-filtration units.

МОДИФІКАЦІЇ КОНСТРУКЦІЇ ТЕПЛООБМІННИХ АПАРАТІВ ТИПУ «ТРУБА В ТРУБІ»

О. К. Василенко — пт, 16 тра 2025 00:00:00 +0300

This article examines recent advancements in enhancing the thermal performance of pipe-in-pipe heat exchangers (PiPHE). By analyzing contemporary research, the study identifies effective strategies such as the use of nanofluids, geometric modifications, and flow disturbance techniques. The findings aim to provide insights into optimizing PiPHE designs for improved heat transfer efficiency.

ТЕРМІЧНЕ МОДЕЛЮВАННЯ ФЕРМЕНТЕРА: ВПЛИВ ОХОЛОДЖЕННЯ НА ЕФЕКТИВНІСТЬ ПРОЦЕСУ

М. А. Воронов — пт, 16 тра 2025 00:00:00 +0300

This work presents thermal simulation of a 10 m³ fermenter under two conditions: with and without a cooling jacket. We assess how the cooling system affects temperature distribution as a key for maintaining optimal microbial growth at 38 – 42 °C.

COMPARATIVE ANALYSIS OF PIPES OF DIFFERENT GEOMETRY IN THE HEATING CHAMBER OF THE EVAPORATOR

N. R. Heraskin, V. P. Kosova — пт, 16 тра 2025 00:00:00 +0300

The novelty of this development lies in the development of a more advanced design of the evaporator using a finned tube in the heating chamber. The work is aimed at using finned heating tubes. The proposed solution allows you to reduce the total number of tubes and, due to the intensification of heat exchange, allows you to reduce the time for the boiling process. Visual visualization of processes in the tubes of the evaporator.

СПОСОБИ ВИЛУЧЕННЯ ГУМІНОВИХ КИСЛОТ ЯК ФУНКЦІОНАЛЬНИХ РЕЧОВИН ДЛЯ ВІДНОВЛЕННЯ ГРУНТІВ

Г. В. Декуша, Л. Ю. Авдєєва, Т. Я. Турчина, А. А. Макаренко — пт, 16 тра 2025 00:00:00 +0300

Promising methods of extracting humic acids from brown coal and peat as sources of humic fertilizers are presented. Besides the basic method of alkaline extraction, various promising methods are described, such as mechanical-chemical extraction, ultrasonic or microwave proсessing, electrolysis, catalytic oxidation, hydrothermal conversion and hydrodynamic cavitation.

MODERN APPROACHES TO THE DESIGN AND OPTIMISATION OF REACTORS FOR PHARMACEUTICAL MANUFACTURING IN THE TRANSITION TO CONTINUOUS PROCESSES AND MICROGRAVITY RESEARCH

R. O. Zipman, V. M. Melnyk — пт, 16 тра 2025 00:00:00 +0300

Different reactor types were examined, including batch reactors whose optimization involved studying equipment design and operating variables—traditionally under the assumption of perfect mixing. To account for non‑idealities in batch reactors, a cell‑model approach was proposed. In continuous processes, plug‑flow (tubular) reactors are widely used, where engineers strive to achieve ideal displacement (plug‑flow) conditions. Separately, biofilm reactors under simulated micro‑gravity were analysed to investigate biofilm growth in spaceflight environments.

STUDY OF ULTRASONIC DESTRUCTION OF MICROALGAE USING A CASCADE EMISSION PLANT

O. O. Kolisnichenko, V. Y. Shybetskyi — пт, 16 тра 2025 00:00:00 +0300

This article investigates the ultrasonic disintegration of microalgae using a cascade emission system. Simulations confirmed effective acoustic pressure and deformation levels for cavitation. Experiments with Chlorella showed that ultrasound power, treatment time, and suspension concentration significantly affect cell destruction. The most intense breakdown occurred within the first minutes at high power and concentration, supporting ultrasound’s application in biofuel production.

РОЗДІЛЕННЯ ПІРОЛІЗНОЇ РІДИНИ З ЗАСТОСУВАННЯМ МЕМБРАННОЇ ТЕХНОЛОГІЇ

Н. К. Коломацький, М. В. Шафарененко — пт, 16 тра 2025 00:00:00 +0300

This work demonstrates the possibility of using membrane technology for the separation of pyrolysis liquid. The research was conducted using a setup equipped with a membrane pervaporation apparatus.

СИСТЕМА НЕІВАЗИВНОГО ВИМІРЮВАННЯ РІВНЯ ГЛЮКОЗИ У КРОВІ З ВИКОРИСТАННЯМ ІНФРАЧЕРВОНОГО ЛАЗЕРУ

А. С. Кулик, О. С. Остапенко, С. О. Мамілов, Ю. П. Горго — пт, 16 тра 2025 00:00:00 +0300

The work is devoted to the development of structural and schematic diagrams of a prototype system for data acquisition and primary processing for measuring blood glucose levels using near-infrared spectroscopy. Studies have shown that there is a clear correlation between the relative increase in photocurrent intensity with increasing blood glucose levels and vice versa, which can be used for non-invasive blood glucose measurement using an IR laser.

DEVELOPMENT OF A METHODOLOGY FOR CALCULATING THE WORKING CHAMBER OF A COMBINED DRYING AND GRINDING PROCESSES EQUIPMENT

A. V. Liashenko — пт, 16 тра 2025 00:00:00 +0300

In Ukraine, the utilization of organic waste from poultry production remains one of the priority tasks of the state environmental policy. Chicken production reaches millions of tons per year [1]. Organic waste also reaches millions of tons per year. The introduction of technologies and equipment is very important for the national economy [2-4]. Organic processing products can be used both in energy plants or as organic fertilizers.

ANALYSIS OF THE EFFICIENCY OF STIRRING THE DEEP CULTURE MEDIUM OF NATIVE MICROBIAL SUSPENSION FOR OBTAINING LACTOBACTERIN

O. V. Martyshev, Z. I. Ostapenko — пт, 16 тра 2025 00:00:00 +0300

This article examines the features of homogenization using different types of mixers: blade, six-blade and open turbine. Using computer modeling with SolidWorks software and the addition of Flow Simulation, mixing processes and the influence of equipment types on the dynamics of a two-component fluid are investigated.

METHODS FOR IMPROVING HEAT EXCHANGE EQUIPMENT IN CITRIC ACID PRODUCTION

O. Moroz, Z. I. Ostapenko — пт, 16 тра 2025 00:00:00 +0300

This paper analyses the main approaches to improving heat exchange equipment used in the process of citric acid production by the microbiological method using fungi of the genus Aspergillus niger. Special attention is paid to modern designs of shell-and-tube heat exchangers, their technical advantages and disadvantages.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ ЗЕРЕН АМАРАНТУ ПІД УЛЬТРАЗВУКОВИМ ВПЛИВОМ

М. А. Мороз, В. М. Мельник — пт, 16 тра 2025 00:00:00 +0300

The effect of ultrasonic waves on the germination process of amaranth seeds was studied. The prospects for the use of amaranth in the food industry, particularly in oil production, were outlined. A significant impact of ultrasonic waves on seed germination was demonstrated, and recommendations for further application were provided.

ЕКСПЕРИМЕНТАЛЬНЕ ВИЗНАЧЕННЯ РОБОЧИХ ХАРАКТЕРИСТИК ТРУБЧАСТИХ ПЕРВАПОРАЦІЙНИХ МЕМБРАН

М. Д. Петрівский, М. В. Шафаренко — пт, 16 тра 2025 00:00:00 +0300

The permeability of pervaporation tubular membranes was experimentally determined and the corresponding diffusion coefficients through the membrane wall were calculated. The dependence of these values on the temperature of the pervaporation process and the thickness of the membrane wall was obtained.

INFLUENCE OF TURBINE IMPELLER BLADE GEOMETRY ON HYDRODYNAMICS IN A BIOREACTOR: NUMERICAL SIMULATION IN ANSYS CFX

A. S. Ruzhanskyi, S. I. Kostyk — пт, 16 тра 2025 00:00:00 +0300

This study investigates the impact of turbine impeller blade geometry (rectangular, circular, triangular, and star-shaped) with identical cross-sectional area and volume on hydrodynamics in a bioreactor using ANSYS CFX. Flow velocity and shear strain rate distributions were analysed to assess mixing efficiency. The rectangular blade exhibited the highest flow velocity but also elevated shear stress, potentially harmful to sensitive cultures, while the triangular blade showed lower shear stress, suitable for delicate microorganisms. Results highlight the importance of blade geometry optimization for enhancing biotechnological processes.

АНАЛІЗ СИСТЕМИ ТЕПЛО ВІДВЕДЕННЯ ПІД ЧАС ПРОЦЕСУ КУЛЬТИВУВАННЯ ЛИМОННОЇ КИСЛОТИ

М. О. Самсоненко — пт, 16 тра 2025 00:00:00 +0300

This paper presents a thermal simulation of a fermenter with a cooling jacket for citric acid cultivation using SolidWorks. The study analysed heat transfer during the first 30 seconds of fermentation. Results showed cooling near the walls, while the centre retained heat due to no mixing. Future improvements include CFD modelling and mixing device integration.

ІННОВАЦІЙНА ЛІНІЯ ДЛЯ ВИРОБНИЦТВА ЦИТРАМОНУ З ВИКОРИСТАННЯМ 3D-МОДЕЛЮВАННЯ РЕАКТОРА-ЗМІШУВАЧА

В. П. Стацюк — пт, 16 тра 2025 00:00:00 +0300

This article presents the design and 3D modeling of a stirred reactor for the pharmaceutical production line of Citramon – a combined analgesic, antipyretic, and anti-inflammatory drug. The mixing stage of active pharmaceutical ingredients is critical for ensuring the uniformity and therapeutic efficacy of the final product. To optimize the mixing process and enhance reactor design, a 3D model of the reactor was developed using Blender software. The reactor consists of a cylindrical shell, elliptical bottom, and flat top cover with an anchor-type stirrer, designed for efficient blending of viscous liquids. A cutaway 3D visualization was created to demonstrate the internal geometry and functionality of the apparatus. The model enables hydrodynamic analysis, identification of stagnant zones, and potential for CFD flow simulations. The developed digital model can be integrated into digital manufacturing systems to support real-time process monitoring and design improvement. The application of 3D modeling in pharmaceutical engineering enhances production quality, process control, and operational safety.

ПРОЕКТУВАННЯ ТА МОДЕЛЮВАННЯ ФЕРМЕНТЕРА

Д. Р. Ткачов — пт, 16 тра 2025 00:00:00 +0300

This article presents the engineering design of a fermenter for the production of benzylpenicillin. Based on biotechnological process requirements, the design provides optimal mixing, aeration, temperature control, and ease of sterilization. A 3D model of the fermenter was created in SolidWorks for volume, geometry, and construction analysis. The result is a vessel suitable for laboratory and pilot-scale production, with the possibility of scaling up for industrial use.

PECULIARITIES OF FLUID MEDIUM PERTURBATION UNDER THE INFLUENCE OF ULTRASONIC RADIATION

S. V. Fesenko, K. Pashchyna — пт, 16 тра 2025 00:00:00 +0300

Most modern biotechnological production facilities face typical challenges during operational processes in equipment used for cultivation, which are related to ensuring effective heat and mass transfer, the presence of turbulent and stagnant zones, high energy consumption, and low heat transfer coefficients when working with viscous fluids. The primary objective of the research was to determine the optimal geometry of the system design for remote influence on the medium in order to control heat and mass transfer processes in bioreactors, as compared to mechanical methods of influence.

INVESTIGATION OF THE INFLUENCE OF SPIRAL BAFFLES ON THE HYDRODYNAMICS IN BIOREACTOR JACKETS

D. S. Khyzhna, S. I. Kostyk — пт, 16 тра 2025 00:00:00 +0300

The study investigates the hydrodynamics of fluid flow in a bioreactor equipped with a conventional jacket featuring spiral baffles. Emphasis is placed on analysing the influence of spiral geometry on flow behaviour and pressure drop, with the goal of optimizing flow conditions to enhance heat transfer efficiency.