Біотехнологія XXI століття

НАУКОВА ДІЯЛЬНІСТЬ КАФЕДРИ ПРОМИСЛОВОЇ БІОТЕХНОЛОГІЇ ТА БІОФАРМАЦІЇ: ДОСЯГНЕННЯ ТА ПЕРСПЕКТИВИ РОЗВИТКУ

2026-05-05T11:37:27+03:00

Кафедра промислової біотехнології та біофармації є одним із провідних науково-освітніх підрозділів у сфері біотехнології, що формує сучасний науковий дискурс та забезпечує підготовку висококваліфікованих фахівців для біотехнологічної, фармацевтичної та харчової галузей. Її становлення розпочалося наприкінці ХХ століття, коли біотехнологія набула статусу однієї з ключових високотехнологічних галузей. Створення у 1990 році відділу біотехнології, а згодом відкриття спеціальності «Біотехнологія» стало важливим кроком у формуванні національної біотехнологічної школи, яка сьогодні активно розвивається та інтегрується у міжнародний науковий простір.

НАУКОВА ДІЯЛЬНІСТЬ КАФЕДРИ БІОЕНЕРГЕТИКИ, БІОІНФОРМАТИКИ ТА ЕКОБІОТЕХНОЛОГІЇ: ДОСЯГНЕННЯ ТА ПЕРСПЕКТИВИ РОЗВИТКУ

2026-05-05T12:06:55+03:00

Кафедра біоенергетики, біоінформатики та екобіотехнології була створена шляхом об’єднання двох кафедр – кафедри екобіотехнології та біоенергетики та кафедри біоінформатики у 2021 році. На кафедрі екобіотехнології та біоенергетики діяла і продовжує свою діяльність і сьогодні наукова школа «Перетворення видів енергії – біоенергетика – екобіотехнологія. За результатами її діяльності напрацьовано науково-технологічні основи конверсії відновлюваної сировини та відходів у біоводень, біометан та біодизель за використання мікроорганізмів та мікроводоростей. Розроблено наукові засади комплексної біотехнології отримання енергоносіїв з одночасною утилізацією діоксиду карбону. Запропоновано розробки нових методів і технологій для очищення природних поверхневих і підземних вод, стічних вод різного походження - промислових, господарсько-побутових, як фізико-хімічними, так і біологічними методами з використанням активних мулів, іммобілізованих мікроорганізмів, асоціацій мікроорганізмів-деструкторів, анаммокс-бактерій. Визначено ефективні методи очищення стічних вод із найменшими економічними і матеріальними витратами при забезпеченні нормативних вимог до якості очищеної води.

НАУКОВА ДІЯЛЬНІСТЬ КАФЕДРИ БІОТЕХНІКИ ТА ІНЖЕНЕРІЇ: ДОСЯГНЕННЯ ТА ПЕРСПЕКТИВИ РОЗВИТКУ

2026-05-05T12:09:58+03:00

Кафедра біотехніки та інженерії (спеціальність G21) здійснює наукову діяльність, спрямовану на дослідження та оптимізацію біотехнологічних процесів із використанням сучасних інженерних і аналітичних підходів. Основу досліджень становить поєднання експериментальних робіт із методами математичного моделювання, що дозволяє комплексно аналізувати функціонування біологічних систем і підвищувати ефективність технологічних процесів.

ЧИСЕЛЬНЕ МОДЕЛЮВАННЯ УЛЬТРАЗВУКОВОЇ ІНТЕНСИФІКАЦІЇ КУЛЬТИВУВАННЯ SCOBY В ФЕРМЕНТЕРІ ЗА ДОПОМОГОЮ МОДУЛЯ ANSYS CFX

2026-05-06T18:55:59+03:00

This study presents a numerical simulation of ultrasonic wave propagation in a 50L cylindrical fermenter with an elliptical bottom designed for kombucha cultivation. Using Ansys CFX, the research analyzed acoustic pressure distribution, velocity fields, and shear strain rates in a specific medium.

ACCELERATING HEAT TRANSFER IN CYLINDRICAL-CONICAL TANKS BY INTENSIFYING COOLANT MOTION

2026-05-06T19:02:28+03:00

The study presents a comparative CFD analysis of two cylindrical-conical tank cooling systems. The first model utilises a standard smooth cooling jacket, while the second incorporates internal baffles to intensify coolant flow. The 5-minute simulation showed that the installation of baffles leads to an increase in the average internal heat transfer coefficient.

АНАЛІЗ ПАРАМЕТРІВ УЛЬТРАЗВУКОВОГО ВПЛИВУ НА ФОРМУВАННЯ ЛІПОСОМАЛЬНИХ НАНОНОСІЇВ ДЛЯ БІОТЕХНОЛОГІЧНИХ ОБ’ЄКТІВ

2026-05-06T19:09:01+03:00

This article analyzes scientific data on ultrasonic parameters for forming stable nanoscale liposomal systems. It examines the impact of power and reactor geometry on cavitation to ensure the protection and delivery of bioactive compounds. Special attention is given to wave behavior and its influence on vesicle stability, measured by electrokinetic potential.

TECHNOLOGICAL SOLUTIONS FOR INDUSTRIAL WASTEWATER TREATMENT TO SUPPORT THE PRINCIPLES OF THE CIRCULAR ECONOMY

2026-05-06T19:17:57+03:00

This paper presents a systematic analysis of recent patent developments in biotechnological industrial wastewater treatment. It covers modular bioelectrocatalytic devices, hybrid biosorption systems, and two-stage anaerobic digestion. Key technical solutions include plug-in bioelectrocatalytic modules (15-35 % organics removal increase), immobilized biomass columns for Li, Co, Ni recovery, and cascade anaerobic-aerobic systems with recirculation.

MODERN APPROACHES TO INDUSTRIAL WASTEWATER TREATMENT

2026-05-06T19:20:32+03:00

This paper analyzes transforming industrial wastewater treatment towards circular biotechnological complexes integrating anaerobic digestion for biogas with biosorption and bioelectrochemical systems to recover energy and recycle heavy metals (Pb, Zn, Cr, Ni, Cu). It highlights advanced synergies using modified consortia, hybrid materials, and digital twins for adaptive control in sustainable production.

ADVANCED METHODS OF PLATE CORRUGATION IN PLATE HEAT EXCHANGERS FOR HEAT TRANSFER INTENSIFICATION

2026-05-06T19:29:34+03:00

This article is devoted to the study of advanced corrugation methods for plates in plate heat exchangers as a means of heat transfer intensification. Special attention is paid to the influence of corrugation geometry on flow structure, turbulence generation, and thermal–hydraulic performance. Modern approaches to optimizing plate profiles to reduce pressure drop while maintaining high efficiency are also considered.

ЧИСЕЛЬНЕ МОДЕЛЮВАННЯ ГІДРОДИНАМІКИ vCSTR-РЕАКТОРА З БАГАТОЯРУСНОЮ МІШАЛКОЮ ДЛЯ ВИРОБНИЦТВА БІОГАЗУ

2026-05-06T19:33:43+03:00

This article presents a 3D CFD simulation of a 2-phase non-Newtonian flow in a large-scale vCSTR biogas reactor using ANSYS CFX. A four-stage mixer efficiency at 15 rpm was studied. Torque reached 29221 N·m (45,9 kW power). Dead zones volume is 45,643 m³. Streamlines and velocity fields confirmed effective mixing without critical shear stress.

ЛІОФІЛІЗАЦІЯ У НАНОМЕДИЦИНІ: РОЗРОБКА ПРОЦЕСІВ, КОНТРОЛЬ ЯКОСТІ ТА ВПРОВАДЖЕННЯ ПРИНЦИПІВ QbD

2026-05-06T19:36:16+03:00

The study examines lyophilization as a method for stabilizing biopharmaceuticals and nanostructured systems. Main process stages and critical quality parameters are analyzed. The importance of Quality by Design and lyoprotectants for reproducibility, structural integrity, and biological activity is highlighted.

DEVELOPMENT AND OPTIMISATION OF NUTRIENT MEDIA COMPOSITION FOR THE CULTIVATION OF ANTIBIOTIC PRODUCERS

2026-05-06T19:39:08+03:00

This study explores the multi-dimensional paradigms of bioprocess engineering, focusing on the statistical and empirical optimization of nutrient media for submerged cultivation of antibiotic-producing microorganismsfrom the genus Streptomyces. Special attention is given to Response Surface Methodology (RSM) and Central Composite Design (CCD). Case studies demonstrate significant yield improvements for chrysomycin A and herbicide 334-W4.

ЗАСТОСУВАННЯ НАНОБІОТЕХНОЛОГІЇ ПРИ СТВОРЕННІ НАНОМЕТРИЧНИХ СТРУКТУР

2026-05-06T19:42:13+03:00

The article presents the results of analytical studies on the potential of nanobiotechnology for the development and successful application of nanometer-scale structures in various industrial fields. A brief overview of different types of nanomaterials and examples of their application in medicine for research, imaging, diagnostics, and treatment is provided.

ІННОВАЦІЇ В ЗАСТОСУВАННІ БІОРЕАКТОРІВ

2026-05-06T19:53:01+03:00

The article presents the results of analytical studies of several examples of innovations in the application of bioreactors. Modern examples of the use of bioreactors in various fields are provided. Different types and designs of bioreactors that are widely used are considered. The results of forecasting the development of the global bioreactor production market are also presented.

CHROMATOGRAPHIC ANALYSIS OF GLYCYRRHIZA GLABRA L. EXTRACTS BY ULTRASOUND-ASSISTED EXTRACTION USING ETHANOL SOLUTIONS OF VARIOUS CONCENTRATIONS

2026-05-06T19:57:13+03:00

This article investigates the influence of ultrasonic treatment and hydroethanolic solvent concentration on the extraction efficiency of biologically active compounds from licorice roots (Glycyrrhiza glabra L.). Differences in the composition of extracts obtained with 40% and 70% ethanol were revealed by thin-layer chromatography. The combination of ultrasonic treatment with 70% ethanol provided the most efficient extraction of biologically active components.

ЛАБОРАТОРІЯ НА ЧИПІ: ПОСТАНОВКА ЗАДАЧІ, ПРОЄКТУВАННЯ ТА ПОПЕРЕДНІ РЕЗУЛЬТАТИ

2026-05-06T19:59:50+03:00

This paper presents the development of a domestic Lab-on-a-Chip system for biomedical research. The microfluidic platform cultivates yeast cells and evaluates growth kinetics in real-time. It uses an ESP32S3-nano, WS2812B LED, and BH1750FVI sensor. Pilot calorimetric studies confirmed system viability, though future thermal stabilization is needed.

АНАЛІЗ КОНСТРУКЦІЙ МІШАЛОК ФЕРМЕНТЕРІВ ДЛЯ НАКОПИЧННЯ БІОМАСИ BACILLUS MEGATERIUM, BACILLUS AMILOLIQUEFACIENS та BACILLUS SUBTILIS У ВИРОБНИЦТВІ БІОПРЕПАРАТІВ

2026-05-06T20:07:13+03:00

The study analyzes fermenter impeller designs for biomass accumulation of Bacillus megaterium, B. amyloliquefaciens and B. subtilis. The final yield of biomass and metabolites is directly affected, in addition to the composition of the medium components, by the design of the apparatus in which the main production process takes place. Comparison and justification of the designs of mixing devices allows us to fully assess the feasibility of a constructive solution for bioreactors.

TWO-PHASE FLOW HYDRODYNAMIC MODELLING IN A FERMENTER FOR CITRIC ACID SYNTHESIS USING THE FROZEN ROTOR APPROACH

2026-05-06T20:10:20+03:00

This work presents a CFD simulation of hydrodynamic processes in a 15 m³ fermenter for citric acid synthesis by Aspergillus niger. Using the Frozen Rotor approach in ANSYS CFX, a two-phase liquid-gas system was modelled with two impellers: a Rushton turbine and a 45° pitched blade impeller. The velocity field, pressure distribution, shear strain rate and air volume fraction were analysed. The results indicate effective mixing throughout the vessel volume with acceptable shear stress levels for microbial cells.

VOF MODELING OF THE TRANSITION BETWEEN DRIPPING AND JETTING REGIMES IN A MICROFLUIDIC GENERATOR

2026-05-08T19:07:19+03:00

Microfluidic droplet generators produce uniform emulsions for oil-based polymerase chain
reaction (oilPCR), where droplet quality depends on the dripping-jetting regime. A Volume of Fluid
(VOF) simulation of a flow-focusing generator with two parametric cases (Ca = 0,066-0,083,
λ=0,97-1,21) shows that flow velocity is the primary factor controlling the regime transition.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ ФОТОАВТОТРОФНИХ МІКРООРГАНІЗМІВ У ЦИКЛІЧНІЙ СИСТЕМІ ПЕРЕРОБКИ ВІДХОДІВ БІОЕНЕРГЕТИКИ

2026-05-06T12:31:26+03:00

This article examines integrating photoautotrophic microorganisms into circular bioenergy systems. It focuses on the bioconversion of bioenergy waste into biomass for biodiesel and plant biostimulants. This transformation turns by-products into valuable resources, completing a sustainable closed-loop bioenergy cycle.

ВПЛИВ МІКРОКОНЦЕНТРАЦІЙ ПЕРЕХІДНИХ МЕТАЛІВ НА КІНЕТИКУ ТЕМНОВОЇ ФЕРМЕНТАЦІЇ БІНАРНИХ СУБСТРАТІВ З МЕТОЮ ОДЕРЖАННЯ БІОВОДНЮ

2026-05-06T12:38:23+03:00

This paper explores iron and nickel ions' effect on biohydrogen production during dark fermentation of apple pomace and chicken manure. The previous thermal shock selected the target microbiome. Adding these metals as hydrogenase cofactors accelerates gas evolution and increases both total biogas volume and hydrogen content. The results confirm the high efficiency of ion modification for biohydrogen intensification.

АСТАКСАНТИН ЯК ПОТУЖНИЙ АНТИОКСИДАНТ

2026-05-06T12:42:09+03:00

This research is devoted to the analysis of astaxanthin as a powerful natural bioactive agent. The study focuses on its ability to neutralize reactive oxygen species and reduce oxidative stress at the cellular level. Special attention is given to prospects for astaxanthin production in Ukraine.

БІОТЕХНОЛОГІЧНІ АСПЕКТИ КУЛЬТИВУВАННЯ HAEMATOCOCCUS PLUVIALIS ЯК ДЖЕРЕЛА АСТАКСАНТИНУ

2026-05-06T12:46:10+03:00

This research provides a comparative analysis of astaxanthin (AST) production efficiency among various microbial sources, identifying Haematococcus pluvialis as the most potent biological produce. The research is primarily focused on the selection and optimization of cultivation conditions

ПРОГРАМНИЙ КОМПЛЕКС АВТОМАТИЗОВАНОЇ ОБРОБКИ ФОТОПЛЕТИЗМОГРАМ ДЛЯ ВИЗНАЧЕННЯ ГЕМОДИНАМІЧНИХ ПАРАМЕТРІВ

2026-05-06T12:49:37+03:00

This paper presents the development of software for automated processing of photoplethysmographic signals for the determination of hemodynamic parameters. The software package developed enables the automated determination of hemodynamic parameters in real time with a delay of one pulse wave. The system’s operation facilitates the rapid assessment of hemodynamic parameters and simplifies the diagnosis and early detection of dysfunctions in the human cardiovascular system.

БІОТЕХНОЛОГІЧНИЙ ПОТЕНЦІАЛ МІКРОВОДОРОСТЕЙ У ПРОЦЕСАХ БІОРЕМЕДІАЦІЇ ВОДНИХ ЕКОСИСТЕМ

2026-05-06T13:01:15+03:00

This paper presents review of the application and potential of microalgae in the bioremediation of aquatic ecosystems. The key mechanisms of pollutant removal are examined, and the effectiveness of microalgae in water purification is evaluated. Also the prospects and limitations of their use in water treatment systems are considered.

ДОВГІ НЕКОДУЮЧІ РНК У ГІПОКСИЧНІЙ ВІДПОВІДІ СЕРЦЕВОЇ ТКАНИНИ ЩУРА: РОЗШИРЕНИЙ АНАЛІЗ ДАНИХ RNA-SEQ ДАТАСЕТУ GSE245300

2026-05-06T13:04:38+03:00

Long non-coding RNAs (lncRNAs) are important regulators of hypoxia-responsive transcription. Here, a lncRNA-focused analysis of the public RNA-seq dataset GSE245300 was performed to identify candidate lncRNAs associated with the hypoxic response in rat cardiac tissue. The results provide a basis for further validation.

ФАКТОРИ ВІРУЛЕНТНОСТІ BORDETELLA BRONCHISEPTICA: ПАТОГЕНОМНИЙ ОГЛЯД РЕФЕРЕНСНОГО ШТАМУ RB50

2026-05-06T13:09:11+03:00

This study presents a bioinformatic analysis of the Bordetella bronchiseptica RB50 genome to identify key virulence factors. Using VFDB and NCBI data, was characterized a comprehensive repertoire of adhesins (FHA, pertactin, fimbriae), toxins (ACT, DNT), and secretion systems (T3SS, T4SS). These findings identify potential genomic markers essential for future diagnostic and vaccine development.

ПЕТЛЕВА ІЗОТЕРМІЧНА АМПЛІФІКАЦІЯ (LAMP): СПЕЦИФІКА ДИЗАЙНУ ПРАЙМЕРІВ

2026-05-06T13:12:23+03:00

This article reviews bioinformatic LAMP primer design, focusing on target selection via sequence alignment. It analyzes thermodynamic properties of the 6-primer set (F3, B3, FIP, BIP, LF, LB) and cross-reactivity. Software and in silico validation are key to increasing diagnostic sensitivity and specificity.

МОДЕЛЮВАННЯ ОКИСНЕННЯ АМОНІЙНОГО АЗОТУ В СТІЧНІЙ ВОДІ В РЕАКТОРАХ ІЗ ЗАКРІПЛЕНОЮ БІОМАСОЮ

2026-05-06T13:18:06+03:00

The study considers a simplified mathematical model of ammonium nitrogen oxidation in a reactor with attached biomass. The dependence of ammonium concentration on time was obtained. The model allows estimation of treatment efficiency and the influence of initial conditions.

ВИКОРИСТАННЯ АЕРОБНИХ ТА АНАЕРОБНИХ ТЕХНОЛОГІЙ ДЛЯ ОЧИЩЕННЯ СТІЧНИХ ВОД РИБОПЕРЕРОБНИХ ПІДПРИЄМСТВ

2026-05-06T13:24:03+03:00

Fish processing belongs to the food industry and generates wastewater with high concentrations of biodegradable organic matter, suspended solids and fats. The paper compares aerobic and anaerobic treatment technologies for fish-processing effluents and substantiates the choice of aerobic treatment for high-flow wastewater due to stable organic matter removal and effective nitrification.

ФЛУОРЕСЦЕНЦІЯ ХЛОРОФІЛУ ЯК ІНСТРУМЕНТ МОНІТОРИНГУ ФОТОСИНТЕТИЧНОЇ АКТИВНОСТІ ТА СТРЕСУ РОСЛИН

2026-05-06T13:33:12+03:00

Chlorophyll fluorescence is a non-invasive tool for assessing plant photosynthetic activity under environmental stress. Using JIP-test and spectral indices (NDVI), the adaptive potential of various plant species was evaluated. The approach enables early detection of stress-induced changes in the photosynthetic apparatus.

АНАЛІЗ СУЧАСНИХ ТЕХНОЛОГІЙ ВИДАЛЕННЯ ФОСФОРУ З МІСЬКИХ СТІЧНИХ ВОД

2026-05-06T13:36:11+03:00

Anthropogenic phosphorus is a major driver of water eutrophication. This study reviews modern approaches to remove phosphorus from urban wastewater and identifies hybrid enhanced biological phosphorus removal combined with chemical polishing is identified as an efficient approach that balances high phosphorus removal efficiency with controlled sludge production.

ВПЛИВ ІОНІВ КУПРУМУ ТА ОСВІТЛЕННЯ НА РІСТ І МЕТАБОЛІЗМ CHLORELLA VULGARIS

2026-05-06T13:39:47+03:00

This study evaluates the combined effects of copper ions and light regimes on growth and metabolism of Chlorella vulgaris. Moderate Cu²⁺ concentrations (0.1–0.5 mg/L) stimulated biomass accumulation, while higher concentrations inhibited pigment synthesis. Red-blue LED lighting enhanced cell growth, whereas dimmed natural light promoted lipid accumulation.

МЕТОДИ ПОЛІМЕРИЗАЦІЇ БІОПЛАСТИКУ ПОЛІЛАКТИДУ З МОЛОЧНОЇ КИСЛОТИ

2026-05-06T13:43:06+03:00

Problems with environmental pollution by plastic waste are serious and need to be solved. Also, raw materials for traditional types of plastic are not renewable. Therefore, there is a need to develop effective methods of producing bioplastics from plant raw materials

ФІТОТОКСИЧНІСТЬ ЙОНІВ Zn2+ Cu2+ Pb2+ Fe3+ ДЛЯ SINAPIS ALBA НА СІРИХ ҐРУНТАХ

2026-05-06T13:47:50+03:00

The phytotoxicity of Zn²⁺, Cu²⁺, Pb²⁺, and Fe³⁺ to Sinapis alba in gray soils was assessed. For Pb²⁺ and Zn²⁺, 20% inhibition of seedling and root growth was observed at 260 and 440 mg/kg, while a 25% decrease in germination occurred at 390 and 660 mg/kg. In soil analogues, Cu²⁺ reduced biomass by 11,0–13,9%, and Fe³⁺ reduced germination by 12–31% and leaf area by 28,6–37,0%.

НАКОПИЧЕННЯ ТА ТОКСИЧНИЙ ВПЛИВ СВИНЦЮ (Pb) НА РОСЛИНИ: МЕХАНІЗМИ ДІЇ ТА МЕТОДИ ЗАХИСТУ

2026-05-06T13:50:53+03:00

Lead (Pb) is a toxic pollutant that disrupts plant growth and photosynthesis. Absorbed via roots and leaves, it triggers internal defense mechanisms. Studying these processes is essential for breeding tolerant crops and improving phytoremediation.

МЕТОД ІНФОРМАЦІЙНОГО ОЦІНЮВАННЯ ХАРАКТЕРОЛОГІЧНИХ ВЛАСТИВОСТЕЙ ОСОБИСТОСТІ

2026-05-06T13:53:44+03:00

This article presents a technology for assessing personality traits based on a qualitative-quantitative approach, which allows developing personalized recommendations for developing a health self-management program and ensures individualization of the selection of rehabilitation interventions. The proposed information technology is implemented in the «TOChO» software module and in the module for selecting recommendation measures. The developed tools have undergone experimental validation.

IN SILICO АНАЛІЗ ТОКСИКОЛОГІЧНОГО ПРОФІЛЮ ТА ПОТЕНЦІЙНИХ ЛІКАРСЬКИХ ВЗАЄМОДІЙ НОВИХ ПОХІДНИХ ТОСУФЛОКСАЦИНУ

2026-05-06T13:57:57+03:00

This study presents an in silico safety assessment of novel tosufloxacin derivatives with bioisosteric substitutions at N1 and C7. ADME/Tox tools were used to predict hepatotoxicity, mutagenicity, P-glycoprotein-related transport and potential drug-drug interactions. The analysis helped identify the most promising derivatives for further study.

INVESTIGATION OF ELECTROGENIC PROPERTIES OF PSEUDOMONAS STRAINS FOR BIOELECTROCHEMICAL APPLICATIONS

2026-05-06T14:01:10+03:00

The present study examines electrogenic characteristics of selected Pseudomonas strains and their potential for further use in microbial fuel cells. Biofilm formation, redox activity and phenazine production were assessed. P. aeruginosa ATCC 27853 showed the highest biofilm formation and phenazine synthesis, highlighting its potential as an efficient electron transfer mediator in bioelectrochemical systems.

АЗОТНИЙ ТА ФОСФОРНИЙ СТРЕС ЯК ЕФЕКТИВНИЙ ПІДХІД ДО СТИМУЛЮВАННЯ НАКОПИЧЕННЯ ЛІПІДІВ У МІКРОВОДОРОСТЯХ

2026-05-06T14:04:15+03:00

This research isexamines Nitrogen and Phosphorus stress and C:N:P stoichiometry's impact on microalgae growth and lipid synthesis. It analyzes the metabolic shift to TAG under nutrient stress and taxonomic nitrogen preferences. The focus is on balancing biomass and lipid content for biofuels.

ПЕРСПЕКТИВИ РОЗВИТКУ ВИРОБНИЦТВА БІОПАЛИВА З МІКРОВОДОРОСТЕЙ: СВІТОВИЙ РИНОК ТА ПЕРСПЕКТИВИ РОЗВИТКУ В УКРАЇНІ

2026-05-06T14:07:51+03:00

This research is devoted to the analysis of microalgae as a promising source for sustainable biofuel production, highlighting their high lipid content, rapid growth and ability to utilize carbon dioxide. Special attention is given to the current challenges of large-scale production and the prospects for developing microalgae-based biofuel technologies in Ukraine.

STRUCTURAL AND GENOMIC FEATURES OF DAUCUS CAROTA α-TUBULIN ASSOCIATED WITH NATURAL RESISTANCE TO DINITROANILINE HERBICIDES

2026-05-06T14:10:52+03:00

Daucus carota is a well-known model of natural resistance to dinitroaniline herbicides, which target α-tubulin and disrupt microtubule assembly. Here, we show through genomic and structural studies that multiple α-tubulin isotypes possess differences in sequence and binding site organization. Using structural modeling, molecular dynamics, and electrostatic analysis, we identified the key steric and electrostatic factors preventing ligand binding.

БІОТЕХНОЛОГІЧНІ МЕТОДИ ПЕРЕРОБКИ ДИГЕСТАТУ МЕТАНТЕНКІВ

2026-05-06T14:15:33+03:00

The article reviews and summarizes the main biotechnological methods of processing digestate from methane tanks. Methods of composting, vermicomposting, cultivation of Hermetia illucens and microalgae are considered. The considered methods allow not only to utilize organic residues after methane fermentation, but also to obtain valuable products.

АНАЛІЗ ТЕХНОЛОГІЙ БІОЛОГІЧНОГО ОЧИЩЕННЯ ПРОМИСЛОВИХ СТІЧНИХ ВОД ВІД ЖИРОВИХ ЗАБРУДНЕНЬ

2026-05-06T14:19:17+03:00

The article analyzes modern biological technologies for the treatment of industrial wastewater contaminated with fats. The advantages, limitations, and application conditions of these methods are discussed to ensure effective wastewater purification.

МОЛЕКУЛЯРНИЙ ДОКІНГ БІОІЗОСТЕРІВ ТОСУФЛОКСАЦИНУ ДО БАКТЕРІАЛЬНИХ ТОПОІЗОМЕРАЗ

2026-05-06T16:40:18+03:00

The molecular docking of 15 tosufloxacin bioisosteres into topoisomerase IV (4KOE) and DNA gyrase (9GGQ) binding sites was performed. The thermodynamic stability of ternary complexes was verified based on binding free energy and metal coordination. Significant affinity was confirmed for the 4KOE target, while steric hindrances in 9GGQ led to ligand displacement. The B4, A1, A11 series candidates were identified as the most promising leaders for further studies.

FROM PLASTIC TO MEDICINE: BIOTECHNOLOGICAL CONVERSION OF PET INTO PARACETAMOL

2026-05-06T16:46:23+03:00

The article examines the global plastic crisis and explores biotechnological conversion of PET waste into paracetamol. It focuses on the use of engineered E. coli, alongside biocompatible reactions, to transform environmental pollutants into beneficial pharmaceuticals, highlighting potential applications in sustainable drug development.

ПОРІВНЯННЯ ТЕХНОЛОГІЙ АНАЕРОБНО-АЕРОБНОГО ОЧИЩЕННЯ СТІЧНИХ ВОД КОНДИТЕРСЬКИХ ПІДПРИЄМСТВ

2026-05-06T16:51:25+03:00

The article analyzes anaerobic-aerobic technologies for treating confectionery wastewater with high organic content. The study compares A/O and A2/O systems, assessing their efficiency in removing organic pollutants and nitrogen compounds. The results highlight the effectiveness of each system under different industrial conditions.

МОЖЛИВІСТЬ ВИКОРИСТАННЯ ІММОБІЛІЗОВАНИХ БАКТЕРІЙ РОДУ BACILLUS ДЛЯ ОЧИЩЕННЯ СТІЧНИХ ВОД ВІД ОРГАНІЧНИХ ЗАБРУДНЕНЬ

2026-05-06T17:07:09+03:00

This paper reviews the use of immobilized Bacillus bacteria, especially Bacillus subtilis, for the treatment of industrial wastewater containing organic pollutants. The analysis focuses on enzymatic degradation processes and shows that immobilization improves stability, reusability, stress resistance, and pollutant removal efficiency compared to free cells.

МЕТАБОЛІЧНА КОНКУРЕНЦІЯ СИНТЕЗУ ЛІПІДІВ І КАРОТИНОЇДІВ У ЧЕРВОНИХ ДРІЖДЖАХ ДЛЯ ОПТИМІЗАЦІЇ БІОПАЛИВА

2026-05-06T17:13:44+03:00

This review analyzes R. glutinis cultivation for biodiesel. Data confirm an antagonistic correlation between lipids and carotenoids. Optimal lipid yield and fatty acid profile (26.68% linoleic acid) are reported at C/N=70 (peptone), C/P=527, and C/S=254. Key conditions include 200 rpm, 30°C, and pH 4.0. Limiting the process to 120h ensures high-quality feedstock with low carotenoids (0.6 mg/L).

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ ІММОБІЛІЗОВАНИХ МІКРООРГАНІЗМІВ ДЛЯ БІОЛОГІЧНОГО ОЧИЩЕННЯ СТІЧНИХ ВОД ГОРІХОПЕРЕРОБНИХ ПІДПРИЄМСТВ

2026-05-06T20:49:26+03:00

The prospects of using immobilized microorganisms on polymer carriers for biological treatment of wastewater from nut-processing enterprises are examined. The influence of the amount of immobilized biomass in the system and contact time on tannin removal efficiency is discussed.

ЕКСПЕРИМЕНТАЛЬНЕ ДОСЛІДЖЕННЯ ФІТОРЕМЕДІАЦІЙНОГО ПОТЕНЦІАЛУ BRASSICA JUNCEA В УМОВАХ ЗАБРУДНЕННЯ ВАЖКИМИ МЕТАЛАМИ

2026-05-06T17:28:58+03:00

The phytoremediation potential of Brassica juncea under Cu, Pb, and Ni stress was evaluated in a 60-day pot experiment. Cu showed high accumulation and low toxicity. Lead significantly inhibited growth, being stabilized in roots (up to 1507 mg/kg). A strong additive toxic effect was found for multi-metal contamination.

ШЛЯХИ ЗНИЖЕННЯ ВАРТОСТІ ФЕРМЕНТАТИВНОГО ГІДРОЛІЗУ В БІОЕНЕРГЕТИЦІ

2026-05-06T18:18:05+03:00

This research paper is devoted to analyzing methods for optimizing the production of lignocellulolytic enzymes for bioenergy purposes. The study focuses on reducing production costs by utilizing agricultural residues as substrates and comparing fermentation techniques.

ПЕРСПЕКТИВНІСТЬ ВИКОРИСТАННЯ МОХІВ SPHAGNUM В БІОЕНЕРГЕТИЦІ ТА ВІДНОВЛЕННІ ЕКОСИСТЕМ

2026-05-06T18:22:09+03:00

Mosses, particularly Sphagnum species, are increasingly recognized as multifunctional biotechnological resources. This review analyzes their role in ecosystem restoration, carbon sequestration, and bioenergy, highlighting microbial fuel cells and thermochemical conversion as sustainable, nature-based solutions.

ВИБІР ПРОДУЦЕНТА ДЛЯ ВИРОБНИЦТВА ІЗОБУТАНОЛУ ІЗ СІЛЬСЬКОГОСПОДАРСЬКИХ ВІДХОДІВ

2026-05-06T18:26:31+03:00

The study focuses on selecting and substantiating the optimal microbial producer for isobutanol synthesis from lignocellulosic biomass. A comparative analysis of the metabolic features of E. coli, S. cerevisiae, and C. glutamicum was conducted. Factors affecting process efficiency, including carbon flux architecture, inhibitor tolerance, and sugar co-utilization, were investigated. The most promising microbial platform for technology scaling was identified.

ПОРІВНЯННЯ ІЗОБУТАНОЛУ З ТРАДИЦІЙНИМИ ТА БІОПАЛИВАМИ

2026-05-06T18:29:20+03:00

The article analyzes isobutanol as a promising alternative fuel, comparing its characteristics with fossil fuels, ethanol, and biodiesel. It focuses on isobutanol's high energy density, infrastructure compatibility, and environmental benefits. The study confirms that isobutanol is an effective component for sustainable energy development.

PREDICTING MAGNETOSOME CHAIN CONFIGURATION USING ENSEMBLE MACHINE LEARNING ALGORITHMS BASED ON GENOMIC DESCRIPTORS

2026-05-06T18:31:41+03:00

Accurate prediction of magnetosome chain architecture is a foundational step in magnetogenetics. We applied a Random Forest classifier to predict chain assembly phenotypes from bacterial genomic profiles. Crucially, genes regulating crystal maturation outperformed traditional cytoskeletal markers as structural predictors. This reveals a profound evolutionary coupling between biomineralization and spatial organization.

VALUE OF STREPTOMYCETES IN SOIL BIOREMEDIATION PROCESSES

2026-05-06T18:34:48+03:00

Streptomycetes are widely distributed in soil and show strong metabolic versatility and stress tolerance, making them key microorganisms for soil bioremediation. This paper reviews their ecological characteristics, degradation of organic pollutants, detoxification of heavy metals, roles in soil microecology, current challenges and future prospects.

ВИКОРИСТАННЯ МЕТАЛООРГАНІЧНИХ КАРКАСНИХ СПОЛУК ДЛЯ ПРЯМОГО ВИЛОВЛЮВАННЯ ВУГЛЕЦЮ З АТМОСФЕРИ

2026-05-06T18:51:45+03:00

This study examines the application of metal-organic frameworks (MOFs) for direct air capture (DAC) of carbon dioxide. Due to their high surface area and tunable structure, MOFs demonstrate high efficiency in CO₂ adsorption at low concentrations. The advantages, limitations, and the prospects for using MOF in DAC systems are analyzed based on modern research.

USE OF MICROORGANISMS AS POTENTIAL AGENTS FOR THE BIOCONTROL OF SORGHUM DISEASES

2026-05-05T12:32:10+03:00

The article examines microorganisms as biocontrol agents for sorghum diseases. It evaluates the antagonistic activity of Pseudomonas synxantha UCM B-399 against Pseudomonas syringae, showing high efficacy with inhibition zones of 20–30 mm. Literature analysis also highlights the effectiveness of Bacillus, Trichoderma, and Azospirillum in plant protection.

БІОТЕХНОЛОГІЧНЕ ОТРИМАННЯ ГЛЮКОЗООКСИДАЗИ ЯК КОМПОНЕНТА АНТИБІОПЛІВКОВИХ ПОКРИТТІВ ДЛЯ МЕДИЧНИХ ІМПЛАНТАТІВ

2026-05-05T12:51:50+03:00

This paper deals with a review of the biotechnological production of glucose oxidase and its anti-biofilm potential. The mechanisms of biofilm destabilization by reaction products are analyzed. The aspects of industrial fermentation, purification methods and integration of the enzyme into medical devices to reduce the risk of infection are described.

ДОСЛІДЖЕННЯ ТРАНСГЕНЕРАЦІЙНОЇ ГЕНОМНОЇ НЕСТАБІЛЬНОСТІ MATRICARIA CHAMOMILLA L. ІНДУКОВАНОЇ РЕНТГЕНІВСЬКИМ ВИПРОМІНЮВАННЯМ

2026-05-05T13:08:07+03:00

This study investigates the transgenerational effects of X-ray irradiation on genomic instability and secondary metabolism in the descendants of Matricaria chamomilla L. Biochemical analysis revealed a "phase opposition" effect: radiation doses that stimulated the synthesis of phenols and flavonoids in parental plants led to their inhibition in the offspring, and vice versa, indicating a wave-like nature of epigenetic memory.

БІОТЕХНОЛОГІЧНІ ПІДХОДИ ДО ОТРИМАННЯ ПРИРОДНИХ БАРВНИКІВ ДЛЯ ВИКОРИСТАННЯ В КОСМЕТОЛОГІЧНИХ ЦІЛЯХ

2026-05-05T13:19:29+03:00

The article summarizes current biotechnological approaches to obtaining natural colorants in cosmetology. Key sources include plants, microbes, and cell cultures. Advantages of natural dyes, including biocompatibility, antioxidant and anti-inflammatory properties. Prospects in decorative and care cosmetics are substantiated.

ТЕХНОЛОГІЧНІ АСПЕКТИ ПРОМИСЛОВОГО КУЛЬТИВУВАННЯ РЕКОМБІНАНТНИХ КЛІТИН-ПРОДУЦЕНТІВ МОНОКЛОНАЛЬНИХ АНТИТІЛ

2026-05-05T13:25:12+03:00

This paper deals with a review of the technological aspects of the industrial cultivation of recombinant cells producing monoclonal antibodies. The key parameters of fed-batch and perfusion bioreactor systems, culture media optimization, and process control strategies are analyzed. The aspects of scaling up the fermentation processes and maintaining product quality attributes to reduce production costs are described.

БІОТЕХНОЛОГІЧНЕ ОТРИМАННЯ ПОЛІМЕРНОЇ ОСНОВИ ДЛЯ ВИРОБНИЦТВА ГІДРОГЕЛІВ ДЛЯ 3D-БІОДРУКУ

2026-05-05T13:29:02+03:00

This article examines the biotechnological synthesis of polymer matrices for biocompatible hydrogels. It focuses on producing bacterial nanocellulose and alginates, including modification strategies for extrusion-based 3D bioprinting. Findings show that microbial synthesis ensures high purity and controlled porosity, significantly boosting cell adhesion and proliferation for regenerative medicine.

THE ANTI-INFLAMMATORY POTENTIAL OF NANO-HYDROXYAPATITES

2026-05-05T13:42:12+03:00

Nanostructured hydroxyapatite is believed to be a promising scaffold material for dental and orthopedic implantation due to its biocompatibility and high osteoconductivity. Recent studies show that nHA has anti-inflammatory properties by modulating immune cell responses and reducing the expression of pro-inflammatory cytokines.

ОГЛЯД СУЧАСНИХ ПЕРОРАЛЬНИХ ФОРМ ІНСУЛІНУ

2026-05-05T13:46:50+03:00

This study evaluates oral insulin as a physiological alternative to injections. Overcoming GI barriers via nanoparticles and microrobots shows preclinical promise. However, clinical candidates face variability and scalability issues. Success depends on integrating advanced nanomedicine to bridge the translational gap.

УЛЬТРАШВИДКІ АНАЛОГИ ІНСУЛІНУ: НОВІ МОЖЛИВОСТІ ГЛІКЕМІЧНОГО КОНТРОЛЮ

2026-05-05T13:59:01+03:00

This study analyzes the pharmacology and clinical efficacy of ultra-rapid-acting insulin analogues. It explains their accelerated absorption mechanisms and highlights their positive impact on postprandial glucose levels and time in range. Furthermore, these analogues optimize hybrid closed-loop systems, advancing physiological insulin replacement without increasing the risk of severe hypoglycemia.

ВПЛИВ ІОНІВ ЦИНКУ ТА ВІТАМІНУ B12 НА РІСТ І ПЕРЕДУМОВИ БІОСИНТЕЗУ ВІТАМІНУ D2 У CHLORELLA VULGARIS

2026-05-05T14:01:49+03:00

The study examines the effects of zinc sulfate and vitamin B₁₂ on the growth and vitamin D₂ biosynthesis in Chlorella vulgaris under combined light (red:blue:green = 8:4:4). Zinc showed a dose-dependent effect, stimulating growth at moderate levels and inhibiting it at higher concentrations. Vitamin B₁₂ enhanced biomass accumulation and stabilized culture growth.

ОТРИМАННЯ РЕКОМБІНАНТНОГО ЛЮДСЬКОГО ІНСУЛІНУ В ТРАНСГЕННИХ РОСЛИНАХ ARABIDOPSIS THALIANA

2026-05-05T14:53:14+03:00

This article investigates the possibility of producing recombinant human insulin using transgenic Arabidopsis thaliana plants. The pSBS4405 expression vector, which encodes oleosin-human insulin (OB-hIN), was used. This technology ensures efficient accumulation of the recombinant protein in oil bodies and simplifies its purification. The insulin obtained after enzymatic maturation meets the physicochemical characteristics and exhibits biological activity in in vivo and in vitro studies.

ФУНКЦІОНАЛЬНА КОМПЛЕМЕНТАРНІСТЬ МЕТОДІВ ДІАГНОСТИКИ MYCOPLASMA HOMINIS

2026-05-05T14:56:56+03:00

This article is devoted to a comparative analysis of the most common diagnostic approaches for Mycoplasma hominis and related species. Based on clinical data and commercially available test kits, the study evaluates functional "niches" of polymerase chain reaction, enzyme linked immunosorbent assay, lateral flow assay and culture methods. The findings emphasize the importance of a complementary approach to ensure balance between diagnostic speed, sensitivity, and clinical depth.

ВПЛИВ рН СЕРЕДОВИЩА НА СКЛАД ЕФІРНОЇ ОЛІЇ, ЩО ПРОДУКУЄТЬСЯ ГРИБОМ EREMOTHECIUM ASHBYI

2026-05-05T15:00:23+03:00

This article is devoted to the analysis of changes in the composition of the essential oil, the ratio of oil components produced by E. ashbyi, depending on the pH of the cultivation medium, and the study of metabolic pathways of the synthesis of aromatic substances in order to find patterns of synthesis between oil components.

РЕГУЛЯЦІЯ ВТОРИННОГО МЕТАБОЛІЗМУ У PENICILLIUM SPP.: ВПЛИВ ДЖЕРЕЛ ВУГЛЕЦЮ ТА АЗОТУ НА СИНТЕЗ ГРИЗЕОФУЛЬВІНУ

2026-05-05T15:12:35+03:00

This review summarizes current data on the effects of carbon and nitrogen sources on griseofulvin synthesis in Penicillium spp. It is shown that lactose and nitrate promote the activation of secondary metabolism, whereas glucose and ammonium inhibit polyketide synthesis via CreA- and AreA-dependent mechanisms.

ОГЛЯД СПОСОБІВ ПОВТОРНОГО ВИКОРИСТАННЯ КЛІТИН ESCHERICHIA COLI ДЛЯ ЦІЛЬНОКЛІТИННОЇ БІОТРАНСФОРМАЦІЇ АМІНОКИСЛОТ

2026-05-05T15:18:40+03:00

Whole-cell biotransformation using E. coli immobilized in calcium alginate beads is an efficient alternative for amino acid production. Using a rotating bed reactor (RBR) ensures catalyst stability, mass transfer, and easy reuse. This scalable method reduces production costs and environmental impact, offering a sustainable industrial solution compared to chemical synthesis.

ВИКОРИСТАННЯ ГІБРИДНИХ TRANSWELL-ОРГАНОЇДНИХ СИСТЕМ У МОДЕЛЮВАННІ БІОЛОГІЧНИХ БАР'ЄРІВ

2026-05-05T15:22:25+03:00

This literature review examines the integration of Transwell systems with organoid technologies and 3D printing for personalized drug screening. It explores the transition from static to dynamic models, highlighting the significance of TEER and P_appmetrics. The research synthesizes global data to propose a novel approach for the Ukrainian biotech sector through the hybridization of barrier models.

LIMITATIONS OF CLASSICAL IN VITRO MODELS FOR ASSESSING THE HYPOCHOLESTEROLEMIC ACTIVITY OF LACTIC ACID BACTERIA

2026-05-05T15:26:25+03:00

These theses are devoted to the analysis of the limitations of classical in vitro models used to assess the hypocholesterolemic activity of probiotic LAB. Particular attention is given to discrepancies between in vitro and in vivo results, which arise from the incomplete consideration of cholesterol-lowering mechanisms, interactions with the intestinal microbiota, and methodological limitations.

ВПЛИВ КРІОСКАРИФІКАЦІЇ НАСІННЯ ПШЕНИЦІ М’ЯКОЇ НА ЙОГО ПРОРОСТАННЯ В УМОВАХ IN VITRO

2026-05-05T15:32:36+03:00

The effect of time of liquid nitrogen scarification on germination and sterility of soft wheat seeds was studied. The seed treatment for 10 min ensured 100% sterility without reducing viability (98–100% germination), indicating its potential for seed preparation.

BIOTECHNOLOGICAL PRODUCTION OF β-CAROTENE FROM DEMETHANOLIZED CRUDE GLYCEROL BY THE OLEAGINOUS RED YEAST SPORIDIOBOLUS PARAROSEUS KM281507: A REVIEW

2026-05-05T15:41:48+03:00

Crude glycerol, a biodiesel by-product, was evaluated as a low-cost carbon source for β-carotene production by the red yeast Sporidiobolus pararoseus KM281507 after methanol removal. Cultivation conditions and scale-up feasibility were assessed, confirming its potential for carotenoid production and biodiesel waste valorization.

БАКТЕРІАЛЬНІ ТА ГРИБНІ ПРОДУЦЕНТИ ЦЕЛЮЛАЗ: ПОРІВНЯЛЬНА ХАРАКТЕРИСТИКА

2026-05-05T15:47:16+03:00

Given the rapidly growing global interest in the effective utilization of renewable lignocellulosic resources, this study provides a comprehensive comparative analysis of bacterial and fungal cellulase producers. Through a systematic review of scientific literature, this work evaluates their biological properties, enzyme synthesis efficiency, and specific advantages or limitations for practical industrial application.

ДОСЛІДЖЕННЯ ЕФЕКТИВНОСТІ ПРОЦЕСУ СУШІННЯ БІОМАСИ ПРОБІОТИЧНИХ КУЛЬТУР РОДІВ LACTOBACILLUS ТА BIFIDOBACTERIUM ЗІ ЗБЕРЕЖЕННЯМ ЇХ ЖИТТЄЗДАТНОСТІ, ПОРІВНЯЛЬНИЙ АНАЛІЗ УКРАЇНСЬКИХ І ЗАРУБІЖНИХ ДОСЛІДЖЕНЬ

2026-05-05T15:52:00+03:00

In this study, a comparative analysis of the effectiveness of the developed protective media with the experimental data of other authors was conducted. The results demonstrate the feasibility of using the developed protective medium, considering the synbiotic effect from the interaction of pro- and prebiotics due to the formation of a milk-inulin matrix.

СУЧАСНІ БІОЛОГІЧНІ АЛЬТЕРНАТИВИ ХІМІЧНИМ ГЕРБІЦИДАМ У СУЧАСНОМУ РОСЛИННИЦТВІ

2026-05-05T15:58:03+03:00

The article reviews and summarizes the negative impact of chemical herbicides on the environment and human health, and systematizes various biological alternatives to weed control in horticultural plants, especially the possibility of bioherbicides, namely phytopathogenic fungi and bacteria, in conducting effective and economically initial methods of control.

БІОФАБРИКАЦІЯ БІМЕТАЛІЧНИХ НАНОЧАСТИНОК Ag-Cu НА ОСНОВІ BACILLUS SUBTILIS ТА BACILLUS AMYLOLIQUEFACIENS І ДОСЛІДЖЕННЯ ЇХ СПЕКТРОФОТОМЕТРИЧНИХ ХАРАКТЕРИСТИК

2026-05-05T16:02:30+03:00

Biogenic synthesis of bimetallic Ag-Cu nanoparticles using Bacillus subtilis strains B-7099, B-7445 and B. amyloliquefaciens strain B-7101 was optimized by altering metal salt addition, resulting in greater reproducibility and peak intensity. UV-Vis spectroscopy (420–440 nm) suggested a potential Cu-core/Ag-shell structure. Future bioactivity studies will clarify their potential for protecting plants against bacterial and fungal phytopathogens.

АНАЛІЗ ФЕРМЕНТАТИВНИХ ШЛЯХІВ БІОСИНТЕЗУ СКЛАДОВИХ ЕФІРНОЇ ОЛІЇ ТРОЯНДИ

2026-05-05T16:08:53+03:00

This study investigates the sequence of biochemical reactions involved in the essential oil synthesis within the fungus Eremothecium. The key components of rose essential oil were analyzed, highlighting the advantages of microbial production as a sustainable alternative to plant extraction. The metabolic pathways for monoterpene synthesis (geraniol, nerol, citronellol) were examined, focusing on the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways.

АНАЛІЗ РОСТУ ШТАМУ LIMOSILACTOBACILLUS FERMENTUM LF-02 НА ЯБЛУЧНОМУ ПЕКТИНІ

2026-05-05T16:15:03+03:00

This research analyzes the effect of apple pectin as the primary carbon source on the growth of Limosilactobacillus fermentum LF-02. Compared to glucose, apple pectin marginally inhibited bacterial growth and resulted in a slightly higher final pH, indicating reduced acidification activity.

ГЕНЕТИЧНА ІНЖЕНЕРІЯ НИТЧАСТИХ ГРИБІВ ДЛЯ ОТРИМАННЯ МІЦЕЛІАЛЬНОГО МАТЕРІАЛУ З ЗАДАНИМИ ХАРАКТЕРИСТИКАМИ

2026-05-05T16:25:50+03:00

This review examines genetic engineering approaches applied to four filamentous fungi — Pleurotus ostreatus, Schizophyllum commune, Aspergillus nidulans, and Trichoderma reesei for producing mycelium-based materials with enhanced properties. Targeted modifications, including gene knockout and CRISPR/Cas9 editing, effectively improved tensile strength, Young's modulus, and hyphal packing density.

DESIGN OF RECOMBINANT IL-10-PRODUCING STRAINS WITH REGULATED EXPRESSION

2026-05-05T16:39:28+03:00

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a key role in regulating immune responses and maintaining immune homeostasis. This study examines current approaches to the development of recombinant microbial strains capable of producing IL-10 using regulated expression systems and inducible promoters for potential biomedical applications.

EFFECT OF EXTRACTANT TYPE ON PHENOLIC COMPOUND YIELD IN ARNICA MONTANA

2026-05-05T16:43:17+03:00

This study evaluates the effect of ethanol concentration on the extraction of phenolic compounds from Arnica montana. Using spectrophotometric analysis, it was determined that the yield of bioactive substances depends significantly on the solvent’s polarity. The maximum recovery of phenolic compounds was achieved with 80% ethanol, providing a methodological basis for optimizing the production of high-quality extracts for pharmaceutical use.

PLANT-BASED ALTERNATIVES TO DAIRY PRODUCTS: OAT MILK POTENTIAL

2026-05-05T16:47:01+03:00

The rising demand for plant-based products has driven the development of fermented plant-based dairy analogues. Oat milk is suitable for fermentation by lactic acid bacteria to improve its nutritional and sensory characteristics. This work focuses on lactic acid fermentation of oat milk, nutritional composition of oat, and the impact fermentation has on smell, texture and digestibility of oat milk.

ЕПІГЕНЕТИЧНА РЕГУЛЯЦІЯ БІОСИНТЕЗУ БЕРБЕРИНУ В КУЛЬТУРАХ РОСЛИННИХ КЛІТИН

2026-05-05T16:53:06+03:00

Berberine is a biologically active isoquinoline alkaloid with a wide range of pharmacological effects. This study considers epigenetic regulation as a mechanism for controlling berberine biosynthesis in plant cell cultures. The role of DNA methylation, histone modifications and stress-induced signaling pathways in activation of secondary metabolism is analyzed.

ВПЛИВ ТЕТ НА ДИФЕРЕНЦІАЦІЮ СТОВБУРОВИХ КЛІТИН

2026-05-05T16:56:59+03:00

Differentiation is a critical process for tissue development during embryogenesis, as well as for tissue regeneration and repair in the adult body. Disruption of TET functions is associated with differentiation defects, changes in transcriptional programs and the development of pathologies, which emphasizes the need for a deeper understanding of the mechanisms of their action.

АСПЕКТИ РОЗРОБКИ ІМУНОФЕРМЕНТНОГО НАБОРУ ДЛЯ СЕРОЛОГІЧНОЇ ДІАГНОСТИКИ ЕХІНОКОКОЗУ

2026-05-05T17:01:02+03:00

The literature on optimisation of indirect ELISA for serological diagnosis of cystic echinococcosis is analysed. Key factors affecting assay reliability, including antigen concentration, serum and conjugate dilutions, incubation time and cut-off values, are identified. The development of a domestic «anti-Echinococcus» ELISA kit is a promising approach.

ПЕРСПЕКТИВИ ВИКОРИСТАННЯ РЕКОМБІНАНТНИХ АНТИГЕНІВ У СЕРОЛОГІЧНІЙ ДІАГНОСТИЦІ ЕХІНОКОКУ

2026-05-05T17:05:16+03:00

A literature review was conducted on current approaches to the serological diagnosis of cystic echinococcosis, with a focus on native and recombinant antigens. The findings demonstrate that the diagnostic performance of antigen-based assays depends on the type of antigen used. The results also support the potential of recombinant antigens for the development of more reliable serological test systems.

КРИТЕРІЇ І СУЧАСНІ ПІДХОДИ ДО ВИБОРУ КЛІТИННИХ МОДЕЛЕЙ

2026-05-05T17:08:33+03:00

Cell models are widely used in biomedical research, but their selection remains challenging. This study systematizes key selection criteria and shows that model complexity should match the research objective. A stepwise use of models improves the reliability and predictive value of results.

AGE-RELATED CHANGES IN FTIR SPECTRAL CHARACTERISTICS OF THE DROSOPHILA MELANOGASTER BODY: COMPARISON OF 3-DAY-OLD AND 11-DAY-OLD IMAGO

2026-05-05T17:12:19+03:00

Age-related changes in FTIR-ATR spectra of Drosophila melanogaster were analyzed using pseudo-Voigt decomposition. Significant spectral shifts between 3- and 11-day-old flies revealed sex-specific aging patterns alongside shared molecular signatures, including protein aggregation and lipid oxidation, highlighting both common and dimorphic aging mechanisms.

АНАЛІЗ СУЧАСНИХ БАКТЕРІАЛЬНИХ БІОПРЕПАРАТІВ ДЛЯ ПОКРАЩЕННЯ ҐРУНТОВОЇ МІКРОБІОТИ

2026-05-05T17:22:19+03:00

These materials analyze the role of agroprobiotic microorganisms in the functioning of soil ecosystems and the stimulation of plant growth. The mechanisms of action of nitrogen-fixing, phosphate-mobilizing, and antagonistic bacteria are considered. Information on the use of microbial consortia and their significance for increasing the efficiency of biological preparations is provided.

PRODUCTION OF YEAST WITH AN INCREASED GLUTATHIONE CONTENT AS AN ANTIOXIDANT SUPPLEMENT FOR LIVESTOCK

2026-05-05T17:26:40+03:00

Glutathione is a key intracellular antioxidant important for reducing oxidative stress in animal production. The study evaluates glutathione biosynthesis in Saccharomyces cerevisiae under cysteine supplementation. A selected strain demonstrated higher efficiency (up to 2,6% GSH) with reduced oxidized forms. The obtained biomass is promising as an antioxidant feed additive.

ЦЕЛЮЛОЛІТИЧНА АКТИВНІСТЬ SCHIZOPHYLLUM COMMUNE НА СЕРЕДОВИЩІ З ДОДАВАННЯМ ЛУШПИННЯ НАСІННЯ СОНЯШНИКА

2026-05-05T17:30:40+03:00

The cellulolytic activity of the basidiomycete Schizophyllum commune was investigated under submerged cultivation conditions using sunflower seed husks as a supplement. It was found that the fungus grew actively in the culture; however, the endoglucanase activity was low. The results indicate that sunflower seed husks weakly induce endoglucanase activity.

ОЦІНКА ПОТЕНЦІАЛУ ШРОТУ ТА МАКУХИ ОЛІЙНИХ КУЛЬТУР ДЛЯ БІОСИНТЕЗУ РИБОФЛАВІНУ EREMOTHECIUM ASHBYI

2026-05-05T17:34:56+03:00

The research focuses on riboflavin production using Ukrainian oilseed processing by-products, including sunflower and soybean cake and sunflower, soybean, and rapeseed meals. Eremothecium ashbyi served as the producer. The composition of substrates affects microbial growth and riboflavin synthesis. The findings demonstrate their suitability for production and feed applications.

ОСНОВНІ ФАКТОРИ ВІРУЛЕНТНОСТІ ERYSIPELOTRIX RHUSIOPATHIAE

2026-05-05T18:36:02+03:00

This review explores E. rhusiopathiae, a pathogen of swine, birds, and humans. It analyzes surface antigens (SpaA/B/C) and adhesive proteins (RspA/B) in colonization and biofilms. Neuraminidase and metabolic adaptations for survival are analyzed. Genomics reveal reductive evolution, positioning it between Firmicutes and Mollicutes.

АНАЛІЗ ВІДОМИХ ШТАМІВ SHIGELLA SPP. ТА ПОЖИВНИХ СЕРЕДОВИЩ ДЛЯ ЇХ КУЛЬТИВУВАННЯ З МЕТОЮ ОТРИМАННЯ ПРЕПАРАТУ ПРОТИ ШИГЕЛЬОЗУ

2026-05-05T18:39:59+03:00

Bacteriophage-based preparations are a promising alternative for the treatment of shigellosis due to increasing antibiotic resistance. The selection of optimal Shigella host strains (S. flexneri, S. sonnei, S. dysenteriae type 1) and a lytic bacteriophage Sfk20 ensures broad-spectrum activity. Optimization of cultivation conditions and nutrient media improves phage production efficiency.

АНАЛІЗ СУЧАСНИХ ІННОВАЦІЙ У ВИРОБНИЦТВІ ТА ЗАСТОСУВАННІ БІОПЕСТИЦИДІВ

2026-05-05T18:44:31+03:00

The review highlights innovations in biopesticide production. It examines the valorization of agri-waste, the use of microbial metabolites, and genetic engineering. Advanced delivery systems are described, including RNA-pesticides on nanocarriers and stimuli-responsive biomass-based composites that react to environmental factors. These approaches ensure environmental safety and the sustainable development of modern agriculture.

КОМБІНАЦІЇ БАКТЕРІАЛЬНИХ ШТАМІВ У ТЕХНОЛОГІЇ СИЛОСУВАННЯ КУКУРУДЗИ: ВПЛИВ НА ФЕРМЕНТАЦІЮ

2026-05-05T18:48:06+03:00

The effect of inoculants with different ratios of homo- and heterofermentative lactic acid bacteria on the quality of corn silage was investigated. It was found that the optimal composition of the inoculant improves the fermentation profile, nutrient retention, and increases the feed value of the silage.

БІОЛОГІЧНА АКТИВНІСТЬ ЛІПОПОЛІСАХАРИДУ PSEUDOMONAS AERUGINOSA

2026-05-05T18:53:04+03:00

This study evaluates the biological activity of lipopolysaccharide (LPS) derived from Pseudomonas aeruginosa as a key virulence factor influencing host immune responses. LPS exhibits strong endotoxic properties, activating innate immunity through Toll-like receptor 4 (TLR4) signaling and inducing the production of proinflammatory cytokines and contributing to systemic inflammatory responses.

АНАЛІЗ СУЧАСНИХ НАПРЯМКІВ ЗАСТОСУВАННЯ НІЗИНУ В БІОМЕДИЦИНІ

2026-05-05T18:57:10+03:00

In this work, the main attention was paid to the food additive E234, which is called nisin, namely the possibilities of its application in other areas. Based on the analysis of the literature, it was determined that nisin can be used for the treatment of various pathological conditions.

БІОТЕХНОЛОГІЧНИЙ ПОТЕНЦІАЛ ВИКОРИСТАННЯ LAETIPORUS SULPHUREUS

2026-05-05T19:03:08+03:00

The work summarizes data on the biotechnological and pharmaceutical potential of Laetiporus sulphureus. The composition of bioactive compounds, pharmacological properties and modern cultivation methods are analyzed. The prospects for using the fungus as a source of natural medicines, functional additives and biotechnological products are outlined.

ПЕРСПЕКТИВНІ ПРОДУЦЕНТИ МОЛОЧНОЇ КИСЛОТИ

2026-05-05T19:09:31+03:00

A number of lactic acid bacteria strains have demonstrated high titer and productivity, demonstrating their potential for use on an industrial scale. To obtain maximum results when using these strains, it is necessary to take into account the composition of the nutrient medium and fermentation conditions.

МОЛЕКУЛЯРНІ МЕХАНІЗМИ ЦИТОТОКСИЧНОГО ТА КАНЦЕРОГЕННОГО ВПЛИВУ МІКРОПЛАСТИКУ НА КЛІТИННІ МОДЕЛІ IN VITRO

2026-05-05T19:13:59+03:00

This study analyzes micro- and nanoplastics (MNPs) effects on in vitro cell cultures, focusing on oxidative stress, mitochondrial dysfunction, and genotoxic outcomes. Reactive oxygen species overproduction, mitochondrial depolarization, impaired oxidative phosphorylation, ATP loss, membrane damage, mitophagy, and additive-driven DNA damage and genetic instability are key mechanisms of injury.

БІОСИНТЕЗ ОКИСНЮВАЛЬНИХ ФЕРМЕНТІВ ПРИ СПІЛЬНОМУ ГЛИБИННОМУ ТА ПОВЕРХНЕВОМУ КУЛЬТИВУВАННІ БАЗИДІОМІЦЕТІВ

2026-05-05T19:18:53+03:00

This study evaluates basidiomycete co-cultivation to modulate oxidative enzyme biosynthesis (laccase, peroxidases). Interspecific antagonism triggers oxidative stress, upregulating laccase and inducing novel isoenzymes without toxic inducers. However, severe spatial and trophic competition represses enzyme synthesis. Optimizing interactions enhances industrial yields.

КРИТЕРІЇ РАЦІОНАЛЬНОГО ПІДБОРУ ПАР БАЗИДІОМІЦЕТІВ ПРИ СПІЛЬНОМУ КУЛЬТИВУВАННІ В УМОВАХ IN VITRO

2026-05-05T19:25:31+03:00

Co-cultivation is a biomimetic strategy to activate silent genes in basidiomycetes. However, the empirical selection of pairs remains a challenge. This review outlines a rational design methodology for microbial consortia based on systematic screening of kinetic growth compatibility and the phenotypic assessment of macroscopic antagonism (deadlock interactions) on solid media.

ДІЯ ПОВЕРХНЕВО-АКТИВНИХ РЕЧОВИН RHODOCOCCUS ERYTHROPOLIS IMB Ac-5017, СИНТЕЗОВАНИХ У РІЗНИХ УМОВАХ КУЛЬТИВУВАННЯ, НА ФІТОПАТОГЕННІ БАКТЕРІЇ

2026-05-05T19:30:03+03:00

This article is devoted to the biological activity of Rhodococcus erythropolis IMV Ac-5017 surfactants synthesized in the presence of a yeast inducer and precursors of phytohormone biosynthesis against bacterial phytopathogens. It was found that surfactants obtained under these conditions showed enhanced antimicrobial and antibiofilm activity against phytopathogens compared with those synthesized in the medium without an inducer.

ДОСЛІДЖЕННЯ ВПЛИВУ МАЛЬТОДЕКСТРИНУ НА РІСТ LACTICASEIBACILLUS RHAMNOSUS LB3

2026-05-05T19:35:29+03:00

The article presents the results of an experimental study of the influence of maltodextrin on the growth of the probiotic strain Lacticaseibacillus rhamnosus LB3. The analysed results demonstrate a decrease in culture growth in maltodextrin medium compared to glucose. These findings emphasize the importance of selecting a prebiotic individually to achieve effective synergy with the probiotic.

ПОТЕНЦІАЛ ВИКОРИСТАННЯ ВТОРИННИХ МЕТАБОЛІТІВ TALAROMAYCES ACULEATUS У ФАРМАЦЕВТИЧНІЙ ПРОМИСЛОВОСТІ

2026-05-05T19:39:45+03:00

The article provides a review of current studies of the pharmaceutical potential of secondary metabolites produced by Talaromyces aculeatus. The analyzed compounds demonstrated a broad spectrum of biological activity, including antidiabetic, antitumor, anti-inflammatory, and antibacterial properties, indicating significant prospects for their application in drug development.

ТЕХНОЛОГІЧНІ АСПЕКТИ ЗАГОТІВЛІ, КРІОКОНСЕРВУВАННЯ ТА КУЛЬТИВУВАННЯ СТОВБУРОВИХ КЛІТИН

2026-05-05T19:42:53+03:00

This paper reviews the main stages of stem cell preparation for clinical use. It describes isolation of hematopoietic and mesenchymal cells, compares enzymatic and explant methods, and reviews freezing techniques, cultivation, and quality control, emphasizing factors that affect cell proliferation and stability.

ВПЛИВ МІКРОБІОМУ ШКІРИ НА ФОРМУВАННЯ ЗАПАХУ ТІЛА ЛЮДИНИ ТА ПЕРСПЕКТИВИ ЙОГО КОРЕКЦІЇ ПРОБІОТИЧНИМИ МІКРООРГАНІЗМАМИ

2026-05-05T19:46:28+03:00

This article is devoted to the study of the influence of the skin microbiome on the formation of human body odors. It describes in detail the peculiarities of the structure of the skin, the diversity of bacteria that inhabit it depending on the area, and the processes of odor formation. It mentions factors that can influence the microbiome, and explores methods for using probiotics and artificial microbial communities to reduce unpleasant odor.

MECHANOTRANSDUCTION-BASED CALCIUM OSCILLATION CONTROL IN ESOPHAGEAL SQUAMOUS CELL CARCINOMA VIA ROTATING MAGNETIC FIELDS AND NANOPARTICLES

2026-05-05T19:52:03+03:00

Rotating magnetic fields can modulate Ca²⁺ oscillation via magnetic nanoparticles as mechanotransducers in malignancies, thus falling below the Ca²⁺ decoding threshold required for Ras/ERK-mediated proliferation. Our ODE model extends KYSE-150 calcium dynamics. Additionally, conserved NOTCH-family mutations across ESCC subtypes support mechanosensitivity disruption as a general carcinogenic feature.

BIO-BASED TERPENE-DERIVED LATEX COATINGS FOR ENHANCED WOOD RESISTANCE TO BIODEGRADATION AND WEATHERING

2026-05-06T01:07:57+03:00

Bio-based latex coatings derived from terpenes are considered as an alternative to petrochemical systems for wood protection. Terpenes are sufficiently available and suitable for polymer synthesis. Such coatings demonstrate enhanced antifungal resistance while maintaining their properties, and their transparency affects photodegradation under UV exposure.

ВПЛИВ РІЗНИХ ДЖЕРЕЛ ВУГЛЕЦЮ ТА АЗОТУ НА СИНТЕЗ АМІЛАЗ ASPERGILLUS NIGER

2026-05-06T01:12:54+03:00

This paper summarizes published data on the effect of carbon and nitrogen sources on amylase synthesis by Aspergillus niger. Literature analysis showed that starch is the most effective carbon source in most studies, while the optimal nitrogen source is strain-specific. Medium optimization is crucial for increasing enzyme production.

CHITIN, CHITIN–GLUCAN COMPLEXES AND CHITOSAN AS ALTERNATIVE TARGET PRODUCTS OF BASIDIOMYCETE BIOMASS PROCESSING

2026-05-06T01:16:34+03:00

This study presents a comparative assessment of chitin, chitin–glucan complexes (CGC), and chitosan derived from basidiomycete biomass. The relationship between processing depth, product yield, functional properties, and environmental impact is analyzed. The results demonstrate that CGC provide the optimal balance between extraction efficiency and functionality, making them the most resource-efficient target product for large-scale applications.

HIDDEN COSTS OF GREEN EXTRACTION: EVALUATION OF E-FACTOR AND WATER FOOTPRINT IN THE RECOVERY OF FUNGAL BIOPOLYMERS

2026-05-06T01:20:43+03:00

This study evaluates the environmental and technological aspects of green extraction of chitin, chitosan, and chitin–glucan complexes from fungal biomass. Key metrics including E-factor, PMI, and water footprint were analyzed. The results indicate that chitin–glucan complexes and intensified processing methods provide the most resource-efficient approach.

СТРАТЕГІЇ ПОКРАЩЕННЯ СТІЙКОСТІ ПРОБІОТИЧНИХ КУЛЬТУР

2026-05-06T01:24:39+03:00

This research paper is devoted to finding the most popular and widely used methods of increasing stability of probiotics. The main aspects of found techniques are explained, analyzed for possible disadvantages and, when rational, compared to each other.

PROSPECTS OF DIGITAL TOOLS FOR APPLIED MICROBIAL BIOTECHNOLOGY

2026-05-06T01:28:15+03:00

This paper is devoted to reviewing the capabilities of digital tools for optimizing development in the field of microbial biotechnology. Comparative analysis of bioinformatic methods, new directions in the study of microorganisms (metabolomics, proteomics, transcriptomics, and others), as well as the development of artificial intelligence technologies show ways to significantly increase the efficiency of developments, reduce the cost of time and resources.

ЗАСТОСУВАННЯ НАНОЧАСТИНОК ДЛЯ ПІДВИЩЕННЯ СТІЙКОСТІ РОСЛИН ДО БІОТИЧНОГО ТА АБІОТИЧНОГО СТРЕСУ В УМОВАХ КЛІМАТИЧНИХ ЗМІН

2026-05-06T01:32:09+03:00

This study examines the use of nanoparticles as a means of enhancing plant resistance to biotic and abiotic stress under climate change conditions. It demonstrates their effectiveness against plant pathogens, as well as their ability to activate plant defense mechanisms. The study highlights the potential of nanotechnology in modern agriculture.

IMPACT OF CROP ROTATION ON SYMBIOTIC NITROGEN FIXATION EFFICIENCY BY BRADYRHIZOBIUM JAPONICUM IN SOYBEAN

2026-05-06T01:35:51+03:00

Crop rotation significantly affects symbiotic nitrogen fixation in soybean by Bradyrhizobium japonicum. This study evaluates how different preceding crops influence nodulation, nitrogen fixation efficiency, and plant growth. The results emphasize the role of crop rotation in improving soil fertility and reducing dependence on synthetic nitrogen fertilizers in sustainable agriculture.

СИНТЕТИЧНІ ТА ПРИРОДНІ МАТРИКСИ ДЛЯ КУЛЬТИВУВАННЯ КЛІТИН

2026-05-06T01:39:10+03:00

Cell culture efficiency is strongly influenced by the properties of scaffolds that mimic the extracellular matrix. Natural matrices provide high biocompatibility and biological activity, while synthetic materials offer controlled mechanical and physicochemical properties. Hybrid systems combining both types represent the most promising approach for advanced cell cultivation applications.

EFFECT OF NIACIN ON THE GROWTH OF THE STRAIN LIMOSILACTOBACILLUS FERMENTUM LF-02

2026-05-06T01:42:37+03:00

The effect of niacin on the growth of strain LF-02 in HM and Base media was investigated. An increase in the number of viable cells with rising niacin concentration was established, with maximum values obtained at an addition level of 0.01 g/dm³. The feasibility of using niacin in LAB cultivation is demonstrated.

SYSTEMATIC EVALUATION OF MOLECULAR GENETIC METHODS FOR SOURDOUGH MICROBIOTA IDENTIFICATION

2026-05-06T01:48:53+03:00

This article is a review of modern molecular-genetic methods for identifying microbiota in sourdough starters, focusing on lactic acid bacteria and yeasts. Comparative evaluation highlights advantages and limitations of PCR-based, restriction, and sequencing approaches, emphasizing the need for a polyphasic strategy to ensure accurate and reproducible microbial characterization.

БЕЗПЕЧНІСТЬ ІНСЕКТИЦИДНИХ БІОПРЕПАРАТІВ НА ОСНОВІ ЕНТОМОПАТОГЕННИХ ГРИБІВ BEAUVERIA BASSIANA ДЛЯ МЕДОНОСНИХ БДЖІЛ APIS MELLIFERA

2026-05-06T01:52:46+03:00

This research evaluates the safety of Beauveria bassiana-based bioinsecticides for honeybee Apis mellifera. Laboratory studies indicate potential mortality at 10⁸-10⁹ conidia/ml, but social immunity and morphological traits significantly mitigate risks in natural colonies. The findings highlight these fungi as a sustainable alternative to chemical pesticides for honeybee Apis mellifera protection.

СТВОРЕННЯ СТАБІЛЬНИХ БІОПОЛІМЕРНИХ КОМПЛЕКСІВ НА ОСНОВІ ХІТОЗАНУ ТА РИБОФЛАВІНУ

2026-05-06T01:55:59+03:00

Chitosan is a linear aminopolysaccharide derived from chitin, widely recognized for its biocompatibility, biodegradability, and low toxicity, making it a promising material for biomedical and industrial applications. Modified with riboflavin chitosan demonstrate improved mechanical stability, antimicrobial activity, and applicability in wound healing, tissue engineering, and food packaging.

ВПЛИВ РІЗНИХ КОНЦЕНТРАЦІЙ ЦИТОКІНІНУ 6-БЕНЗИЛАМІНОПУРИНУ НА РЕГЕНЕРАЦІЮ ПАГОНІВ У КУЛЬТУРІ ЗРІЛИХ ЗАРОДКІВ ПШЕНИЦІ СОРТУ ПОДОЛЯНКА

2026-05-06T01:59:58+03:00

This study investigates the effect of various 6-benzylaminopurine (BAP) concentrations on the shoot regeneration frequency in mature embryo culture of the common wheat Podolyanka cultivar. It was shown that the efficiency of shoot regeneration did not significantly depend on the BAP concentration in the regeneration medium after callus induction on MS medium supplemented with 2 mg/l 2.4-D.