INVESTIGATION OF ELECTROGENIC PROPERTIES OF PSEUDOMONAS STRAINS FOR BIOELECTROCHEMICAL APPLICATIONS
Ключові слова:
Pseudomonas spp., biofilm formation, optical density, DREAM coefficient, redox potential.Анотація
The present study examines electrogenic characteristics of selected Pseudomonas strains and their potential for further use in microbial fuel cells. Biofilm formation, redox activity and phenazine production were assessed. P. aeruginosa ATCC 27853 showed the highest biofilm formation and phenazine synthesis, highlighting its potential as an efficient electron transfer mediator in bioelectrochemical systems.
Посилання
Yang L., Deng W., Zhang Y. et al. Boosting current generation in microbial fuel cells by an order of magnitude by coating an ionic liquid polymer on carbon anodes. Biosensors and Bioelectronics. 2017. Vol. 91. P. 644–649. DOI: 10.1016/j.bios.2017.01.028
Qiao Y. J., Qiao Y., Zou L. et al. Biofilm promoted current generation of Pseudomonas aeruginosa microbial fuel cell via improving the interfacial redox reaction of phenazines. Bioelectrochemistry. 2017. Vol. 117. P. 34–39. DOI: 10.1016/j.bioelechem.2017.04.003
Kragh K. N., Alhede M., Kvich L., Bjarnsholt T. Into the well—A close look at the complex structures of a microtiter biofilm and the crystal violet assay. Biofilm. 2019. Vol. 1. P. 100-106. DOI: 10.1016/j.bioflm.2019.100006
Aiyer K. S., Rai R., Vijayakumar B. S. Dye reduction-based electron-transfer activity monitoring assay for assessing microbial electron transfer activity of microbial fuel cell inocula. Journal of Environmental Sciences. 2020. Vol. 96. P. 171–177. DOI: 10.1016/j.jes.2020.04.037
Mavrodi D. V., Peever T. L., Mavrodi O. V. et al. Diversity and evolution of the phenazine biosynthesis pathway. Appl Environ Microbiol. 2010. Vol. 76, № 3. P. 866-879. DOI: 10.1128/AEM.02009-09
Bosire E. M., Rosenbaum M. A. Electrochemical Potential Influences Phenazine Production, Electron Transfer and Consequently Electric Current Generation by Pseudomonas aeruginosa. Front Microbiol. 2017. Vol. 8. P. 892-900. DOI: 10.3389/fmicb.2017.00892
Wang N., Newman D. K. Redox reactions of phenazine antibiotics with ferric (hydr)oxides and molecular oxygen. Environ. Sci. Technol. 2008. Vol. 42. P. 2380-2386. DOI:10.1021/es702290a
Franco A., Elbahnasy M., Rosenbaum M. A. Screening of natural phenazine producers for electroactivity in bioelectrochemical systems. MicrobBiotechnol. 2023. Vol. 16, № 3, P. 579–594. DOI: 10.1111/1751-7915.14199
