CONDITIONED MEDIUMS IN REGENERATIVE MEDICINE
Ключові слова:
mesenchymal stromal cells, conditioned medium, cellular pre-conditioning, regenerative medicineАнотація
Conditioned medium (CM), a bioactive cell culture supernatant, has emerged as a promising cell-free therapeutic strategy in regenerative medicine. This review examines CM’s complex composition, production methods, mechanisms of action, and applications in tissue repair and immunomodulation, particularly from MSCs. It also addresses delivery strategies, regulatory issues, and challenges in clinical translation.
Посилання
Mesenchymal stem cell secretome: toward cell-free therapeutic strategies in regenerative medicine / F. Vizoso et al. International Journal of Molecular Sciences. 2017. Vol. 18, no. 9. P. 1852. URL: https://doi.org/10.3390/ijms18091852.
Mesenchymal-stromal-cell-conditioned media and their implication for osteochondral regeneration / D. Ivanisova et al. International Journal of Molecular Sciences. 2023. Vol. 24, no. 10. P. 9054. URL: https://doi.org/10.3390/ijms24109054.
Gingiva-derived mesenchymal stem cells: potential application in tissue engineering and regenerative medicine - a comprehensive review / D. Kim et al. Frontiers in Immunology. 2021. Vol. 12. URL: https://doi.org/10.3389/fimmu.2021.667221.
Conditioned medium of mesenchymal stem cells pretreated with H2O2 promotes intestinal mucosal repair in acute experimental colitis / P. Liu et al. Scientific Reports. 2022. Vol. 12, no. 1. URL: https://doi.org/10.1038/s41598-022-24493-y.
Mesenchymal stem cell-conditioned media-loaded microparticles enhance acute patency in silk-based vascular grafts / K. L. Lorentz et al. Bioengineering. 2024. Vol. 11, no. 9. P. 947. URL: https://doi.org/10.3390/bioengineering11090947.
Photobiomodulation and mesenchymal stem cell-conditioned medium for the repair of experimental critical-size defects / M. Bayat et al. Lasers in Medical Science. 2024. Vol. 39, no. 1. URL: https://doi.org/10.1007/s10103-024-04109-9.
Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37 / A. Krasnodembskaya et al. STEM CELLS. 2010. Vol. 28, no. 12. P. 2229–2238. URL: https://doi.org/10.1002/stem.544.
Conditioned medium – is it an undervalued lab waste with the potential for osteoarthritis management? / M. A. Rosochowicz et al. Stem Cell Reviews and Reports. 2023. URL: https://doi.org/10.1007/s12015-023-10517-1.
Effects of mesenchymal stem cells conditioned medium treatment in mice with cholestatic liver fibrosis / D. Pinheiro et al. Life Sciences. 2021. P. 119768. URL: https://doi.org/10.1016/j.lfs.2021.119768.
Mesenchymal stromal cell secretome: influencing therapeutic potential by cellular pre-conditioning / J. R. Ferreira et al. Frontiers in Immunology. 2018. Vol. 9. URL: https://doi.org/10.3389/fimmu.2018.02837.
Conditioned medium of mesenchymal stem cells pretreated with H2O2 promotes intestinal mucosal repair in acute experimental colitis / P. Liu et al. Scientific Reports. 2022. Vol. 12, no. 1. URL: https://doi.org/10.1038/s41598-022-24493-y.
Pawitan J. A. Prospect of stem cell conditioned medium in regenerative medicine. BioMed Research International. 2014. Vol. 2014. P. 1–14. URL: https://doi.org/10.1155/2014/965849.
Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic, and degenerative intervertebral discs: a proteomic analysis / S. Wangler et al. Stem Cell Research & Therapy. 2021. Vol. 12, no. 1. URL: https://doi.org/10.1186/s13287-020-02062-2.
Caplan A. I., Dennis J. E. Mesenchymal stem cells as trophic mediators. Journal of Cellular Biochemistry. 2006. Vol. 98, no. 5. P. 1076–1084. URL: https://doi.org/10.1002/jcb.20886.
Jarmalaviciute A., Pivoriūnas A. Neuroprotective properties of extracellular vesicles derived from mesenchymal stem cells. Neural Regeneration Research. 2016. Vol. 11, no. 6. P. 0. URL: https://doi.org/10.4103/1673-5374.184480.
Smolinská V., Boháč M., Danišovič Ľ. Current status of the applications of conditioned media derived from mesenchymal stem cells for regenerative medicine. Physiological Research. 2023. P. S233–S245. URL: https://doi.org/10.33549/physiolres.935186.
