ІНДУКУВАННЯ НАДСИНТЕЗУ РИБОФЛАВІНУ EREMOTHECIUM ASHBYI РЕГУЛЯЦІЄЮ МЕТАБОЛІЧНИХ ШЛЯХІВ
Ключові слова:
Eremothecium ashbyi, riboflavin, itaconate, UV-irradiationАнотація
Eremothecium ashbyi is a natural producer of riboflavin, an essential water-soluble vitamin involved in redox reactions as a precursor of FMN and FAD. The study aimed to enhance riboflavin biosynthesis via UV irradiation and itaconate treatment. The highest yield of 531,61 mg/L was obtained on oil-based media, indicating the effectiveness of stress-induced stimulation and metabolic regulation.
Посилання
Lienhart W.-D., Gudipati V., Macheroux P. The human flavoproteome. Archives of Biochemistry and Biophysics. 2013. Vol. 535, no. 2. P. 150–162. URL: https://doi.org/10.1016/j.abb.2013.02.015.
Pinto J. T., Cooper A. J. L. From cholesterogenesis to steroidogenesis: role of riboflavin and flavoenzymes in the biosynthesis of vitamin D. Advances in Nutrition. 2014. Vol. 5, no. 2. P. 144–163. URL: https://doi.org/10.3945/an.113.005181.
Kodali V. K., Thorpe C. Oxidative protein folding and the quiescin–sulfhydryl oxidase family of flavoproteins. Antioxidants & Redox Signaling. 2010. Vol. 13, no. 8. P. 1217–1230. https://doi.org/10.1089/ars.2010.3098.
Production of vitamin B2 (riboflavin) by microorganisms: an overview / L. A. Averianova et al. Frontiers in Bioengineering and Biotechnology. 2020. Vol. 8. URL: https://doi.org/10.3389/fbioe.2020.570828.
Aguiar T. Q., Silva R., Domingues L. Ashbya gossypii beyond industrial riboflavin production: a historical perspective and emerging biotechnological applications. Biotechnology Advances. 2015. Vol. 33, no. 8. P. 1774–1786. URL: https://doi.org/10.1016/j.biotechadv.2015.10.001.
Abbas C. A., Sibirny A. A. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers. Microbiology and Molecular Biology Reviews. 2011. Vol. 75, no. 2. P. 321–360. URL: https://doi.org/10.1128/mmbr.00030-10.
Kalingan A. E., Liao C.-M. Influence of type and concentration of flavinogenic factors on production of riboflavin by Eremothecium ashbyii NRRL 1363. Bioresource Technology. 2002. Vol. 82, no. 3. P. 219–224. URL: https://doi.org/10.1016/s0960-8524(01)00194-8.
Shimizu S. Vitamins and related compounds: microbial production. Biotechnology set. Wiley-VCH, Weinheim. 2001. Р. 322–326.
Metabolic flux analysis in Ashbya gossypii using 13C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions / S. K. Schwechheimer et al. Microbial Cell Factories. 2018. Vol. 17, no. 1. URL: https://doi.org/10.1186/s12934-018-1003-y.
Stahmann K. P., Revuelta J. L., Seulberger H. Three biotechnical processes using Ashbya gossypii, Candida famata, or Bacillus subtilis compete with chemical riboflavin production. Applied Microbiology and Biotechnology. 2000. Vol. 53, no. 5. P. 509–516. URL: https://doi.org/10.1007/s002530051649.
