ОСОБЛИВОСТІ ВИБОРУ РОСЛИННИХ ПРОДУЦЕНТІВ ДЛЯ СИНТЕЗУ РЕКОМБІНАНТНИХ ТЕРАПЕВТИЧНИХ БІЛКІВ

Автор(и)

  • Ю. Р. Удовенко КПІ ім. Ігоря Сікорського, Україна
  • О. О. Овчаренко КПІ ім. Ігоря Сікорського; Інститут клітинної біології та генетичної інженерії НАН України, Україна

Ключові слова:

plant molecular farming, transgenic plants, recombinant pharmaceutical proteins

Анотація

Molecular farming is a promising technology for producing recombinant therapeutic proteins in plants in large volumes without the risk of accumulation of animal pathogens. This paper discusses the advantages and disadvantages of different plant species used for molecular farming, identifying optimal species for the production of recombinant proteins by transient expression, stable nuclear, and chloroplast transformation.

Посилання

Shanmugaraj B., I. Bulaon C. J., Phoolcharoen W. Plant molecular farming: a viable platform for recombinant biopharmaceutical production. Plants. 2020. Vol. 9, no. 7. P. 842. URL: https://doi.org/10.3390/plants9070842.

Exploring recent progress of molecular farming for therapeutic and recombinant molecules in plant systems / J. K. Bharathi et al. Heliyon. 2024. Vol. 10, no. 18. P. e37634. URL: https://doi.org/10.1016/j.heliyon.2024.e37634.

Gene-Editing technologies and applications in legumes: progress, evolution, and future prospects / M. C. Baloglu et al. Frontiers in Genetics. 2022. Vol. 13. URL: https://doi.org/10.3389/fgene.2022.859437.

Burnett M. J. B., Burnett A. C. Therapeutic recombinant protein production in plants: challenges and opportunities. PLANTS, PEOPLE, PLANET. 2019. Vol. 2, no. 2. P. 121–132. URL: https://doi.org/10.1002/ppp3.10073.

Smýkal P., von Wettberg E. J. B., McPhee K. Legume genetics and biology: from mendel’s pea to legume genomics. International Journal of Molecular Sciences. 2020. Vol. 21, no. 9. P. 3336. URL: https://doi.org/10.3390/ijms21093336.

Nivya V. M., Shah J. M. Recalcitrance to transformation, a hindrance for genome editing of legumes. Frontiers in Genome Editing. 2023. Vol. 5. URL: https://doi.org/10.3389/fgeed.2023.1247815.

Genome editing in cereals: approaches, applications and challenges / W. A. Ansari et al. International Journal of Molecular Sciences. 2020. Vol. 21, no. 11. P. 4040. URL: https://doi.org/10.3390/ijms21114040.

Buyel J. F. Product safety aspects of plant molecular farming. Frontiers in Bioengineering and Biotechnology. 2023. Vol. 11. URL: https://doi.org/10.3389/fbioe.2023.1238917.

Facts, uncertainties, and opportunities in wheat molecular improvement / F. Rafiei et al. Heredity. 2024. URL: https://doi.org/10.1038/s41437-024-00721-1.

Gerszberg A. Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview. Planta. 2018. Vol. 248, no. 5. P. 1037–1048. URL: https://doi.org/10.1007/s00425-018-2961-3.

Establishment of a stable, effective and universal genetic transformation technique in the diverse species of Brassica oleracea / X. Sheng et al. Frontiers in Plant Science. 2022. Vol. 13. URL: https://doi.org/10.3389/fpls.2022.1021669.

Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants / N. Shcherbak et al. Frontiers in Plant Science. 2023. Vol. 14. URL: https://doi.org/10.3389/fpls.2023.1271757.

Robust agrobacterium-mediated transient expression in two duckweed species (Lemnaceae) directed by non-replicating, replicating, and cell-to-cell spreading vectors / A. Peterson et al. Frontiers in Bioengineering and Biotechnology. 2021. Vol. 9. URL: https://doi.org/10.3389/fbioe.2021.761073.

Sindarovska Y., Kuchuk M. Viral-based expression cassettes ensure high level production of recombinant green fluorescent protein (GFP) in sweet basil (Ocimum basilicum) plants. Plant Cell, Tissue and Organ Culture (PCTOC). 2023. URL: https://doi.org/10.1007/s11240-023-02516-4.

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Опубліковано

2025-05-16