REGULATION OF CRISPR-CAS TYPE III ACTIVITY BY MEMBRANE-ASSOCIATED PROTEINS WITH CARF AND SAVED DOMAINS

Автор(и)

  • Y. Vasylchenko Educational and Scientific Center "Institute of Biology and Medicine" Taras Shevchenko Kyiv National University, Ukraine
  • V. Malinauskaite Vilnius University Life Sciences Center, Lithuania

Анотація

The type III CRISPR-Cas system is a prokaryotic adaptive immune system that confers immunity against invading mobile genetic elements (MGEs). This system involves the use of a multi-subunit effector complex to target foreign DNA transcripts complementary to a guide/CRISPR RNA (crRNA). Type III CRISPR-Cas has an additional layer of protection that includes the production of signaling molecules and effector proteins that respond to them. This system provides a wide range of potential defense pathways involving nucleases, transcription factors, proteases, deaminases, and membrane-associated proteins. Therefore, tight and specific regulation of the activation of accessory proteins has evolved, exhibiting properties such as signal amplification, autoregulation, and tuning the concentration of signaling molecules.

Посилання

Steens J. A., Salazar C. R. P., Staals R. H. J. The diverse arsenal of type III CRISPR–Casassociated CARF and SAVED effectors. Biochemical Society Transactions. 2022. URL: https://doi.org/10.1042/bst20220289

Shmakov S. A., Makarova K. S., Wolf Y. I., Severinov K. V., Koonin E. V. Systematic prediction of genes functionally linked to CRISPR-Cas systems by gene neighborhood analysis. Proceedings of the National Academy of Sciences. 2018. URL: https://doi.org/10.1073/pnas.1803440115

Beljouw S. P. B., Sanders J., Rodríguez-Molina A., Brouns S. J. J. RNA-targeting CRISPR–Cas systems. Nature Reviews Microbiology. 2023. URL: https://doi.org/10.1038/s41579-022-00793-y

##submission.downloads##

Опубліковано

2023-05-22