CLASSIFICATION OF VECTORS FOR GENE DELIVERY IN EUKARYOTIC CELLS

Автор(и)

  • N. O. Snihur Igor Sikorsky Kyiv Polytechnic Institute, Ukraine
  • P. S. Kovalchuk Igor Sikorsky Kyiv Polytechnic Institute, Ukraine
  • B. V. Morgun Igor Sikorsky Kyiv Polytechnic Institute, Ukraine

Анотація

A eukaryotic vector (EV) is a small DNA molecule capable of autonomic replication in animal cells. In addition to nucleotide sequences (NS) that ensure replication, EV should contain genes being used as selectable markers. It has one or more unique restriction sites to insert DNA being cloned. Since the direct cloning of recombinant DNA in animal cells would be an expensive and inefficient procedure, the EV is utilized to express already cloned heterologous NS in cells, while the cloning process itself is carried out in bacteria. For expression in eukaryotic cells, recombinant DNA is placed under the control of particular regulatory elements, which are recognized by the fermentative system of the host. The aim of this article is to outline and classify different vectors that can be applied for the delivery of genes.

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Опубліковано

2023-05-18 — Оновлено 2023-05-22